Protective Effect of Paulownia tomentosa Fruits in an Experimental Animal Model of Acute Lung Injury
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초록
The fruits of Paulownia tomentosa (Thunb.) (PT) Steud. have been reported to exert a variety of biological activities. A previous study confirmed that compounds isolated from PT fruits (PTF) exerted anti-inflammatory effects on TNF-α-stimulated airway epithelial cells. However, there is no report on the protective effects of PTF on acute lung injury (ALI). Here, we examined the ameliorative effects of PTF in an experimental animal model of lipopolysaccharide (LPS)-induced ALI. In ALI mice, increased levels of inflammatory cell influx were confirmed in the lungs of mice, and an increase of microphage numbers, TNF-α, IL-6 and MCP-1 production and protein content were detected in mouse bronchoalveolar lavage fluid. However, these increases were significantly reversed with PTF pretreatment. In addition, PTF inhibited the increased expression of iNOS and COX-2 in the lungs of ALI mice. Furthermore, the upregulation of MAPK and NF-κB activation was decreased in the lungs of ALI mice by PTF. In the in vitro experiment, PTF pretreatment exerted an anti-inflammatory effect by inhibiting the secretion of nitric oxide, TNF-α and IL-6 in LPS-stimulated RAW264.7 macrophages. Collectively, these results indicated that PTF has ameliorative effects on airway inflammation in an experimental animal model of ALI.
Abstract
The fruits of Paulownia tomentosa (Thunb.) (PT) Steud. have been reported to exert a variety of biological activities. A previous study confirmed that compounds isolated from PT fruits (PTF) exerted anti-inflammatory effects on TNF-α-stimulated airway epithelial cells. However, there is no report on the protective effects of PTF on acute lung injury (ALI). Here, we examined the ameliorative effects of PTF in an experimental animal model of lipopolysaccharide (LPS)-induced ALI. In ALI mice, increased levels of inflammatory cell influx were confirmed in the lungs of mice, and an increase of microphage numbers, TNF-α, IL-6 and MCP-1 production and protein content were detected in mouse bronchoalveolar lavage fluid. However, these increases were significantly reversed with PTF pretreatment. In addition, PTF inhibited the increased expression of iNOS and COX-2 in the lungs of ALI mice. Furthermore, the upregulation of MAPK and NF-κB activation was decreased in the lungs of ALI mice by PTF. In the in vitro experiment, PTF pretreatment exerted an anti-inflammatory effect by inhibiting the secretion of nitric oxide, TNF-α and IL-6 in LPS-stimulated RAW264.7 macrophages. Collectively, these results indicated that PTF has ameliorative effects on airway inflammation in an experimental animal model of ALI.
참고문헌 (28)
- Li J, "The receptor for advanced glycation end products mediates dysfunction of airway epithelial barrier in a lipopolysaccharides-induced murine acute lung injury model", Int. Immunopharmacol, 93, pp.107419~ (2021)
- Lee JW, "The role of macrophages in the development of acute and chronic inflammatory lung diseases", Cells, 10, pp.897~ (2021)
- Bhargava M, "Biomarkers in acute lung injury", Transl. Res, 159, pp.205~217 (2012)
- Li H, "miR26a5p alleviates lipopolysaccharideinduced acute lung injury by targeting the connective tissue growth factor", Mol. Med. Rep, 23, pp.5~ (2021)
- Laskin DL, "Role of macrophages in acute lung injury and chronic fibrosis induced by pulmonary toxicants", Toxicol. Sci, 168, pp.287~301 (2019)
- Min Jae-Hong, "Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation", Journal of Microbiology and Biotechnology, 31(11), pp.1501~1507 (2021), 한국미생물·생명공학회[DOI]
- Guimaraes LMF, "Implications of SARS-Cov-2 infection on eNOS and iNOS activity : Consequences for the respiratory and vascular systems", Nitric Oxide, 111-112, pp.64~71 (2021)
- Giuliano F, "Origins of prostaglandin E2 : involvements of cyclooxygenase(COX)-1 and COX-2 in human and rat systems", J. Pharmacol. Exp. Ther, 303, pp.1001~1006 (2002)
- 박지원, "The Anti-Inflammatory Effect of Trichilia martiana C. DC. in the Lipopolysaccharide- Stimulated Inflammatory Response in Macrophages and Airway Epithelial Cells and in LPS-Challenged Mice", Journal of Microbiology and Biotechnology, 30(11), pp.1614~1625 (2020), 한국미생물·생명공학회[DOI]
- Ding H, "Chicoric acid alleviates lipopolysaccharide-induced acute lung injury in mice through anti-inflammatory and anti-oxidant activities", Int. Immunopharmacol, 66, pp.169~176 (2019)
- Yang S, "The natural product bergenin ameliorates lipopolysaccharideinduced acute lung injury by inhibiting NF-κB activition", J. Ethnopharmacol, 200, pp.147~155 (2017)
- Min JH, "3, 4, 5-Trihydroxycinnamic acid exerts a protective effect on pulmonary inflammation in an experimental animal model of COPD", Int. Immunopharmacol, 85, pp.106656~ (2020)
- Lee JW, "3, 4, 5-Trihydroxycinnamic acid attenuates lipopolysaccharide(LPS)-induced acute lung injury via downregulating inflammatory molecules and upregulating HO-1", AMPK activation, 2018, pp.64, 123~130
- Navratilova A, "Minor C-geranylated flavanones from Paulownia tomentosa fruits with MRSA antibacterial activity", Phytochemistry, 89, pp.104~113 (2013)
- Ryu HW, "Potential anti-inflammatory effects of the fruits of Paulownia tomentosa", J. Nat. Prod, 80, pp.2659~2665 (2017)
- Ali SA, "The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine : initiated and promoted hepatocarcinogenesis", Heliyon, 5, pp.e01769~ (2019)
- Lee JW, "Biflavonoid-rich fraction from Daphne pseudomezereum var. koreana Hamaya exerts anti-inflammatory effect in an experimental animal model of allergic asthma", J. Ethnopharmacol, 265, pp.113386~ (2021)
- Kim SM, "Methyl p-hydroxycinnamate exerts anti-inflammatory effects in mouse models of lipopolysaccharide-induced ARDS", Mol. Med. Rep, 25, pp.37~ (2022)
- Kim SM, "Callicarpa japonica Thunb. ameliorates allergic airway inflammation by suppressing NF-κB activation and upregulating HO-1 expression", J. Ethnopharmacol, 267, pp.113523~ (2021)
- Kwon OK, "DK-1108 exerts anti-inflammatory activity against phorbol 12-myristate 13-acetate-induced inflammation and protective effect against OVA-induced allergic asthma", Biomed. Pharmacother, 132, pp.110950~ (2020)
- Huang X, "The role of macrophages in the pathogenesis of ALI", ARDS, 2018, pp.2018, 1264913~
- Chen X, "Macrophage polarization and its role in the pathogenesis of acute lung injury", acute respiratory distress syndrome, 2020, pp.69, 883~895
- He Q, "Apelin36protects against lipopolysaccharide-induced acute lung injury by inhibiting the ASK1", MAPK signaling pathway, 2021, pp.23, 6~
- Yang L, "Cardamonin inhibits LPS-induced inflammatory responses and prevents acute lung injury by targeting myeloid differentiation factor 2", Phytomedicine, 93, pp.153785~ (2021)
- Badamjav R, "The protective effects of Thalictrum minus L. on lipopolysaccharideinduced acute lung injury", J. Ethnopharmacol, 248, pp.112355~ (2020)
- Ryu HW, "Daphnodorin C isolated from the stems of Daphne kiusiana Miquel attenuates airway inflammation in a mouse model of chronic obstructive pulmonary disease", Phytomedicine, 96, pp.153848~ (2022)
- 이재원, "Compound K ameliorates airway inflammation and mucus secretion through the regulation of PKC signaling in vitro and in vivo", Journal of Ginseng Research, 46(3), pp.496~504 (2022), 고려인삼학회[DOI]
- Kim MO, "Black ginseng extract suppresses airway inflammation induced by cigarette smoke and lipopolysaccharides in vivo", Antioxidants, 11, pp.679~ (2022)