Pine Needle Extract Applicable to Topical Treatment for the Prevention of Human Papillomavirus Infection
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초록
Most cervical cancers are associated with high-risk human papillomavirus (HPV) infection. Currently, cervical cancer treatment entails surgical removal of the lesion, but treatment of infection and preventing tissue damage are issues that still remain to be addressed. Herbal medicine and biological studies have focused on developing antiviral drugs from natural sources. In this study, we analyzed the potential antiviral effects of Pinus densiflora Sieb. et Zucc. leaf extracts against HPV. The pine needle extracts from each organic solvent were analyzed for antiviral activity. The methylene chloride fraction (PN-MC) showed the highest activity against HPV pseudovirus (PV). The PN-MC extract was more effective before, rather than after treatment, and therefore represents a prophylactic intervention. Mice were pre-treated with PN-MC via genital application or oral administration, followed by a genital or subcutaneous challenge with HPV PV, respectively. The HPV challenge results showed that mice treated via genital application exhibited complete protection against HPV. In conclusion, PN-MC represents a potential topical virucide for HPV infection.
Abstract
Most cervical cancers are associated with high-risk human papillomavirus (HPV) infection. Currently, cervical cancer treatment entails surgical removal of the lesion, but treatment of infection and preventing tissue damage are issues that still remain to be addressed. Herbal medicine and biological studies have focused on developing antiviral drugs from natural sources. In this study, we analyzed the potential antiviral effects of Pinus densiflora Sieb. et Zucc. leaf extracts against HPV. The pine needle extracts from each organic solvent were analyzed for antiviral activity. The methylene chloride fraction (PN-MC) showed the highest activity against HPV pseudovirus (PV). The PN-MC extract was more effective before, rather than after treatment, and therefore represents a prophylactic intervention. Mice were pre-treated with PN-MC via genital application or oral administration, followed by a genital or subcutaneous challenge with HPV PV, respectively. The HPV challenge results showed that mice treated via genital application exhibited complete protection against HPV. In conclusion, PN-MC represents a potential topical virucide for HPV infection.
참고문헌 (31)
- Bray F, "Global cancer statistics 2018 : GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries", CA Cancer J. Clin, 68, pp.394~424 (2018)
- Lembo D, "Sulfated K5 Escherichia coli polysaccharide derivatives as wide-range inhibitors of genital types of human papillomavirus", Antimicrob. Agents Chemother, 52, pp.1374~1381 (2008)
- Small W, Jr, "Cervical cancer : A global health crisis", Cancer, 123, pp.2404~2412 (2017)
- Li N, "Human papillomavirus type distribution in 30, 848 invasive cervical cancers worldwide : Variation by geographical region, histological type and year of publication", Int. J. Cancer, 128, pp.927~935 (2011)
- Donalisio M, "Identification of a dendrimeric heparan sulfate-binding peptide that inhibits infectivity of genital types of human papillomaviruses", Antimicrob. Agents Chemother, 54, pp.4290~4299 (2010)
- Stanley MA, "Genital human papillomavirus infections : current and prospective therapies", J. Gen. Virol, 93, pp.681~691 (2012)
- Moscicki AB, "Impact of HPV infection in adolescent populations", J. Adolesc. Health, 37, pp.S3~9 (2005)
- Wang SX, "Potential anti-HPV and related cancer agents from marine resources : an overview", Mar. Drugs, 12, pp.2019~2035 (2014)
- Cha MK, "Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16", BMC Med, 10, pp.72~ (2012)
- Underwood MR, "Searching for antiviral drugs for human papillomaviruses", Antivir. Ther, 5, pp.229~242 (2000)
- Ahn WS, "Protective effects of green tea extracts(polyphenon E and EGCG)on human cervical lesions", Eur. J. Cancer Prev, 12, pp.383~390 (2003)
- Fradet-Turcotte A, "Recent advances in the search for antiviral agents against human papillomaviruses", Antivir. Ther, 12, pp.431~451 (2007)
- 이병훈, "Coptidis Rhizoma extract inhibits replication of respiratory syncytial virus in vitro and in vivo by inducing antiviral state", The Journal of Microbiology, 55(6), pp.488~498 (2017), 한국미생물학회
- Iljazovic E, "Efficacy in treatment of cervical HRHPV infection by combination of beta interferon, and herbal therapy in woman with different cervical lesions", Bosn. J. Basic Med. Sci, 6, pp.79~84 (2006)
- Polansky H, "Human papillomavirus(HPV) : systemic treatment with Gene-Eden-VIR", Novirin safely and effectively clears virus, 2017, pp.11, 575~583
- Miyoshi N, "Applications of a standardized green tea catechin preparation for viral warts and human papilloma virus-related and unrelated cancers", Molecules, 25, pp.2588~ (2020)
- Ha TKQ, "Antiviral activities of compounds isolated from pinus densiflora(Pine Tree)against the influenza A Virus", Biomolecule, 10, pp.711~ (2020)
- Zou K, "Two diastereomeric saponins with cytotoxic activity from Albizia julibrissin", Carbohydr. Res, 324, pp.182~188 (2000)
- Buck CB, "Efficient intracellular assembly of papillomaviral vectors", J. Virol, 78, pp.751~757 (2004)
- Pastrana DV, "Reactivity of human sera in a sensitive, highthroughput pseudovirus-based papillomavirus neutralization assay for HPV16 and HPV18", Virology, 321, pp.205~216 (2004)
- Buck CB, "Arrangement of L2 within the papillomavirus capsid", J. Virol, 82, pp.5190~5197 (2008)
- Buck CB, "Production of papillomavirus-based gene transfer vectors", Curr. Protoc. Cell Biol (2007)
- Johnson KM, "Role of heparan sulfate in attachment to and infection of the murine female genital tract by human papillomavirus", J. Virol, 83, pp.2067~2074 (2009)
- Buck CB, "Maturation of papillomavirus capsids", J. Virol, 79, pp.2839~2846 (2005)
- Conway MJ, "Tissue-spanning redox gradient-dependent assembly of native human papillomavirus type 16 virions", J. Virol, 83, pp.10515~10526 (2009)
- Lee HJ, "Immunogenicity of bivalent human papillomavirus DNA vaccine using human endogenous retrovirus envelope-coated baculoviral vectors in mice and pigs", PLoS One, 7, pp.e50296~ (2012)
- Roberts JN, "Genital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan", Nat. Med, 13, pp.857~861 (2007)
- Longet S, "A murine genital-challenge model is a sensitive measure of protective antibodies against human papillomavirus infection", J. Virol, 85, pp.13253~13259 (2011)
- Singh O, "Microbicides for the treatment of sexually transmitted HIV Infections", J. Pharm. (Cairo), 2014, pp.352425~ (2014)
- Calagna G, "‘Secondary prevention’ against female HPV infection : literature review of the role of carrageenan", Expert Rev. Anti. Infect. Ther, 18, pp.865~874 (2020)
- Lee C, "Carrageenans as broad-spectrum microbicides : currentstatus and challenges", Mar. Drugs, 18, pp.435~ (2020)