Actinidia arguta Sprout as a Natural Antioxidant: Ameliorating Effect on Lipopolysaccharide-Induced Cognitive Impairment
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Here, we investigated the prebiotic and antioxidant effects of Actinidia arguta sprout water extract (AASWE) on lipopolysaccharide (LPS)-induced cognitive deficit mice. AASWE increased viable cell count, titratable acidity, and acetic acid production in Lactobacillus reuteri strain and showed a cytoprotective effect on LPS-induced inflammation in HT-29 cells. We assessed the behavior of LPSinduced cognitive deficit mice using Y-maze, passive avoidance and Morris water maze tests and found that administration of AASWE significantly improved learning and memory function. The AASWE group showed antioxidant activity through downregulation of malondialdehyde levels and upregulation of superoxide dismutase levels in brain tissue. In addition, the AASWE group exhibited activation of the cholinergic system with decreased acetylcholinesterase activity in brain tissue. Furthermore, AASWE effectively downregulated inflammatory mediators such as phosphorylated- JNK, phosphorylated-NF-κB, TNF-α and interleukin-6. The major bioactive compounds of AASWE were identified as quercetin-3-O-arabinopyranosyl(1→2)-rhamnopyranosyl(1→6)-glucopyranose, quercetin-3-O-apiosyl(1 →2)-galactoside, rutin, and 3-caffeoylquinic acid. Based on these results, we suggest that AASWE not only increases the growth of beneficial bacteria in the intestines, but also shows an ameliorating effect on LPS-induced cognitive impairment.
Abstract
Here, we investigated the prebiotic and antioxidant effects of Actinidia arguta sprout water extract (AASWE) on lipopolysaccharide (LPS)-induced cognitive deficit mice. AASWE increased viable cell count, titratable acidity, and acetic acid production in Lactobacillus reuteri strain and showed a cytoprotective effect on LPS-induced inflammation in HT-29 cells. We assessed the behavior of LPSinduced cognitive deficit mice using Y-maze, passive avoidance and Morris water maze tests and found that administration of AASWE significantly improved learning and memory function. The AASWE group showed antioxidant activity through downregulation of malondialdehyde levels and upregulation of superoxide dismutase levels in brain tissue. In addition, the AASWE group exhibited activation of the cholinergic system with decreased acetylcholinesterase activity in brain tissue. Furthermore, AASWE effectively downregulated inflammatory mediators such as phosphorylated- JNK, phosphorylated-NF-κB, TNF-α and interleukin-6. The major bioactive compounds of AASWE were identified as quercetin-3-O-arabinopyranosyl(1→2)-rhamnopyranosyl(1→6)-glucopyranose, quercetin-3-O-apiosyl(1 →2)-galactoside, rutin, and 3-caffeoylquinic acid. Based on these results, we suggest that AASWE not only increases the growth of beneficial bacteria in the intestines, but also shows an ameliorating effect on LPS-induced cognitive impairment.
참고문헌 (45)
- Duerkop BA, "Immune responses to the microbiota at the intestinal mucosal surface", Immunity, 31, pp.368~376 (2009)
- Nicholson JK, "Host-gut microbiota metabolic interactions", Science, 336, pp.1262~1267 (2012)
- Caracciolo B, "Cognitive decline, dietary factors and gut–brain interactions", Mech. Ageing Dev, 136, pp.59~69 (2014)
- Noble EE, "Gut to brain dysbiosis : Mechanisms linking western diet consumption, the microbiome, and cognitive impairment", Front. Behav. Neurosci, 11, pp.9~ (2017)
- Jia S, "Chitosan oligosaccharides alleviate cognitive deficits in an amyloid-β1–42-induced rat model of Alzheimer’s disease", Int. J. Biol. Macromol, 83, pp.416~425 (2016)
- Yen CH, "Fructo-oligosaccharide improved brain β-amyloid, β-secretase, cognitive function, and plasma antioxidant levels in D-galactose-treated Balb", cJ mice, 2016, pp.20, 228~237
- Singh TP, "Characterization of intestinal Lactobacillus reuteri strains as potential probiotics", Probiotics Antimicrob. Proteins, 4, pp.47~58 (2012)
- Cryan JF, "The microbiome-gut-brain axis : From bowel to behavior", Neurogastroenterol. Motil, 23, pp.187~192 (2011)
- Lee YJ, "Epigallocatechin-3-gallate prevents systemic inflammation-induced memory deficiency and amyloidogenesis via its anti-neuroinflammatory properties", J. Nutr. Biochem, 24, pp.298~310 (2013)
- 임현우, "Anti-Oxidative and Nitric Oxide Production Inhibitory Activities of PhenolicCompounds from the Fruits of Actinidia arguta", Natural Product Sciences, 12(4), pp.221~225 (2006), 한국생약학회
- Ha JS, "Antiamnesic effect of Actinidia arguta extract intake in a mouse model of TMT-induced learning and memory dysfunction", Evid. -Based Complement. Altern. Med, 2015, pp.876484~ (2015)
- Kim HY, "Extracts of Actinidia arguta stems inhibited LPS-induced inflammatory responses through nuclear factor-κB pathway in Raw 264.7 cells", Nutr. Res, 34, pp.1008~1016 (2014)
- 이아연, "Hypoglycemic and antioxidant effects of Daraesoon (Actinidia arguta shoot) in animal models of diabetes mellitus", Nutrition Research and Practice, 9(3), pp.262~267 (2015), 한국영양학회[DOI]
- Qiao Y, "Effects of different Lactobacillus reuteri on inflammatory and fat storage in high-fat diet-induced obesity mice model", J. Funct. Food, 14, pp.424~434 (2015)
- Reza MA, "In vitro prebiotic effects and quantitative analysis of Bulnesia sarmienti extract", J. Food Drug Anal, 24, pp.822~830 (2016)
- He W, "On-line HPLC-ABTS screening and HPLC-DAD-MS", MS identification of free radical scavengers in Gardenia(Gardenia jasminoides Ellis)fruit extracts, 2010, pp.123, 521~528
- Hvattum E, "Determination of phenolic compounds in rose hip(Rosa canina)using liquid chromatography coupled to electrospray ionisation tandem mass spectrometry and diode-array detection", Rapid Commun. Mass Spectrom, 16, pp.655~662 (2010)
- Khallouki F, "Comparison of the major polyphenols in mature argan fruits from two regions of Morocco", Food Chem, 221, pp.1034~1040 (2017)
- Ye M, "Characterization of phenolic compounds in the Chinese herbal drug Tu-Si-Zi by liquid chromatography coupled to electrospray ionization mass spectrometry", Rapid Commun. Mass Spectrom, 19, pp.1469~1484 (2005)
- Bravo JA, "Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve", Proc. Natl. Acad. Sci. USA, 108, pp.16050~16055 (2011)
- Savignac HM, "Bifidobacteria exert strain-specific effects on stress-related behavior and physiology in BALB", c mice, 2014, pp.26, 1615~1627
- Gareau MG, "Probiotic treatment of rat pups normalises corticosterone release and ameliorates colonic dysfunction induced by maternal separation", Gut, 56, pp.1522~1528 (2007)
- Parkar SG, "Evaluation of the prebiotic potential of five kiwifruit cultivars after simulated gastrointestinal digestion and fermentation with human faecal bacteria", Int. J. Food Sci. Technol, 53, pp.1203~1210 (2017)
- Duary RK, "Immunomodulatory activity of two potential probiotic strains in LPS-stimulated HT-29 cells", Genes Nutr, 9, pp.398~ (2014)
- Kanauchi O, "Germinated barley foodstuff, a prebiotic product, ameliorates inflammation of colitis through modulation of the enteric environment", J. Gastroenterol, 38, pp.134~141 (2003)
- Saulnier DM, "The intestinal microbiome, probiotics and prebiotics in neurogastroenterology", Gut Microbes, 4, pp.17~27 (2013)
- Patil CS, "Protective effect of flavonoids against aging-and lipopolysaccharide-induced cognitive impairment in mice", Pharmacology, 69, pp.59~67 (2003)
- Padurariu M, "Changes of some oxidative stress markers in the serum of patients with mild cognitive impairment and Alzheimer's disease", Neurosci. Lett, 469, pp.6~10 (2010)
- Hsia CH, "Fructo-oligosaccharide systemically diminished D-galactose-induced oxidative molecule damages in BALB", cJ mice, 2012, pp.107, 1787~1792
- Tyagi E, "Influence of LPS-induced neuroinflammation on acetylcholinesterase activity in rat brain", J. Neuroimmunol, 205, pp.51~56 (2008)
- 임유진, "Physiological Components of Kiwifruits with In vitro Antioxidant and Acetylcholinesterase Inhibitory Activities", Food Science and Biotechnology, 23(3), pp.943~949 (2014), 한국식품과학회[DOI]
- Goujon E, "Adrenalectomy enhances pro-inflammatory cytokines gene expression, in the spleen, pituitary and brain of mice in response to lipopolysaccharide", Mol. Brain Res, 36, pp.53~62 (1996)
- Roth J, "Fever induction pathways : Evidence from responses to systemic or local cytokine formation", Braz. J. Med. Biol. Res, 34, pp.301~314 (2001)
- Banks WA, "Blood-brain barrier transport of cytokines : A mechanism for neuropathology", Curr. Pharm. Design, 11, pp.973~984 (2005)
- Savignac HM, "Prebiotic administration normalizes lipopolysaccharide(LPS)-induced anxiety and cortical 5-HT2A receptor and IL1-β levels in male mice", Brain Behav. Immun, 52, pp.120~131 (2016)
- Shokryazdan P, "Effects of prebiotics on immune system and cytokine expression", Med. Microbiol. Immunol, 206, pp.1~9 (2017)
- Qiao Y, "Chitosan oligosaccharides suppressant LPS binding to TLR4", MD-2 receptor complex, 2010, pp.82, 405~411
- Kim HP, "Anti-inflammatory plant flavonoids and cellular action mechanisms", J. Pharmacol. Sci, 96, pp.229~245 (2004)
- Hou Y, "Anti-depressant natural flavonols modulate BDNF and beta amyloid in neurons and hippocampus of double TgAD mice", Neuropharmacology, 58, pp.911~920 (2010)
- Sergent T, "Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium", Chem. Biol. Interact, 188, pp.659~667 (2010)
- Scalbert A, "Absorption and metabolism of polyphenols in the gut and impact on health", Biomed. Pharmacother, 56, pp.276~282 (2002)
- Wang D, "Role of intestinal microbiota in the generation of polyphenol-derived phenolic acid mediated attenuation of Alzheimer's disease β-amyloid oligomerization", Mol. Nutr. Food Res, 59, pp.1025~1040 (2015)
- Etxeberria U, "Impact of polyphenols and polyphenolrich dietary sources on gut microbiota composition", J. Agric. Food Chem, 61, pp.9517~9533 (2013)
- China R, "Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus", Microbiol. Res, 167, pp.500~506 (2012)
- Comalada M, "In vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-κB pathway", Eur. J. Immunol, 35, pp.584~592 (2005)
