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Bioconversion of Untreated Corn Hull into L-Malic Acid by Trifunctional Xylanolytic Enzyme from Paenibacillus curdlanolyticus B-6 and Acetobacter tropicalis H-1

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초록

L-Malic acid (L-MA) is widely used in food and non-food products. However, few microorganisms have been able to efficiently produce L-MA from xylose derived from lignocellulosic biomass (LB). The objective of this work is to convert LB into L-MA with the concept of a bioeconomy and environmentally friendly process. The unique trifunctional xylanolytic enzyme, PcAxy43A from Paenibacillus curdlanolyticus B-6, effectively hydrolyzed xylan in untreated LB, especially corn hull to xylose, in one step. Furthermore, the newly isolated, Acetobacter tropicalis strain H1 was able to convert high concentrations of xylose derived from corn hull into L-MA as the main product, which can be easily purified. The strain H1 successfully produced a high L-MA titer of 77.09 g/l, with a yield of 0.77 g/g and a productivity of 0.64 g/l/h from the xylose derived from corn hull. The process presented in this research is an efficient, low-cost and environmentally friendly biological process for the green production of L-MA from LB.

Abstract

L-Malic acid (L-MA) is widely used in food and non-food products. However, few microorganisms have been able to efficiently produce L-MA from xylose derived from lignocellulosic biomass (LB). The objective of this work is to convert LB into L-MA with the concept of a bioeconomy and environmentally friendly process. The unique trifunctional xylanolytic enzyme, PcAxy43A from Paenibacillus curdlanolyticus B-6, effectively hydrolyzed xylan in untreated LB, especially corn hull to xylose, in one step. Furthermore, the newly isolated, Acetobacter tropicalis strain H1 was able to convert high concentrations of xylose derived from corn hull into L-MA as the main product, which can be easily purified. The strain H1 successfully produced a high L-MA titer of 77.09 g/l, with a yield of 0.77 g/g and a productivity of 0.64 g/l/h from the xylose derived from corn hull. The process presented in this research is an efficient, low-cost and environmentally friendly biological process for the green production of L-MA from LB.

참고문헌 (44)

  1. Baramee S, "Alkaline xylanolytic–cellulolytic multienzyme complex from the novel anaerobic alkalithermophilic bacterium Cellulosibacter alkalithermophilus and its hydrolysis of insoluble polysaccharides under neutral and alkaline conditions", Process Biochem, 50, pp.643~650 (2015)
  2. Pauly M, "Cell-wall carbohydrates and their modification as a resource for biofuels", Plant J, 54, pp.559~568 (2008)
  3. Zhou L, "The impact of fine particulate matter (PM2.5) on China’s agricultural production from 2001 to 2010", J. Clean. Prod, 178, pp.133~141 (2018)
  4. Barl B, "Combined chemical and enzymic treatments of corn husk lignocellulosics", J. Sci. Food Agric, 56, pp.195~214 (1991)
  5. Jönsson LJ, "Pretreatment of lignocellulose : formation of inhibitory by-products and strategies for minimizing their effects", Bioresour. Technol, 199, pp.103~112 (2016)
  6. Kumar P, "Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production", Ind. Eng. Chem. Res, 48, pp.3713~3729 (2009)
  7. Teeravivattanakit T, "Chemical pretreatment-independent saccharifications of xylan and cellulose of rice straw by bacterial weak lignin-binding xylanolytic and cellulolytic enzymes", Appl. Environ. Microbiol, 83, pp.e01522~17 (2017)
  8. Kövilein A, "Malic acid production from renewables : a review", J. Chem. Technol. Biotech, 95, pp.513~526 (2019)
  9. Chi Z, "Microbial biosynthesis and secretion of L-malic acid and its applications", Crit. Rev. Biotechnol, 36, pp.99~107 (2016)
  10. Deng Y, "Metabolic engineering of a laboratory-evolved Thermobifida fusca muC strain for malic acid production on cellulose and minimal treated lignocellulosic biomass", Biotechnol. Prog, 32, pp.14~20 (2016)
  11. Cheng C, "Polymalic acid fermentation by Aureobasidium pullulans for malic acid production from soybean hull and soy molasses : fermentation kinetics and economic analysis", Bioresour. Technol, 223, pp.166~174 (2017)
  12. Giorno L, "Study of an enzyme membrane reactor with immobilized fumarase for production of L-malic acid", Biotechnol. Bioeng, 72, pp.77~84 (2001)
  13. Teeravivattanakit T, "Novel trifunctional xylanolytic enzyme Axy43A from Paenibacillus curdlanolyticus strain B-6 exhibiting endo-xylanase, β-D-xylosidase, and arabinoxylan arabinofuranohydrolase activities", Appl. Environ. Microbiol, 82, pp.6942~6951 (2016)
  14. Duong HTBl, "Identification of Acetorbactor tropicalis for malic acid production", Proceeding of the 14th South East Asian Technical University Consortium 2020 (SEATUC 2020)International Conference, pp.123~127 (2020)
  15. Nelson N, "A photometric adaptation of the Somogyi method for the determination of glucose", J. Biol. Chem, 153, pp.375~380 (1944)
  16. Sluiter A, "Determination of structural carbohydrates and lignin in biomass", Lab. Anal. Procedure, 1617, pp.1~16 (2008)
  17. Zou X, "Production of polymalic acid and malic acid from xylose and corncob hydrolysate by a novel Aureobasidium pullulans YJ 6–11 strain", Process Biochem, 51, pp.16~23 (2016)
  18. Van Dyk J, "A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes—factors affecting enzymes, conversion and synergy", Biotechnol. Adv, 30, pp.1458~1480 (2012)
  19. Wong KK, "Trichoderma xylanases, their properties and application", Crit. Rev. Biotechnol, 12, pp.413~435 (1992)
  20. Khuenkaeo N, "Production and characterization of bio-oil and biochar from ablative pyrolysis of lignocellulosic biomass residues", Chem. Eng. Commun, 207, pp.153~160 (2020)
  21. Mardawati E, "The enzymatic hydrolysis of oil palm empty fruit bunches to xylose", J. Jpn. Inst. Energy, 93, pp.973~978 (2014)
  22. Jommuengbout P, "Substrate-binding site of family 11 xylanase from Bacillus firmus K-1 by molecular docking", Biosci. Biotechnol. Biochem, 73, pp.833~839 (2009)
  23. Ye Y, "Production and characteristics of a novel xylose-and alkali-tolerant GH 43 β-xylosidase from Penicillium oxalicum for promoting hemicellulose degradation", Sci. Rep, 7, pp.1~11 (2017)
  24. Kumar V, "Bioconversion of pentose sugars to value added chemicals and fuels : Recent trends, challenges and possibilities", Bioresour. Technol, 269, pp.443~451 (2018)
  25. Tachaapaikoon C, "Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column", Process Biochem, 41, pp.2441~2445 (2006)
  26. Baramee S, "Biological pretreatment of rice straw with cellulase-free xylanolytic enzyme-producing Bacillus firmus K-1 : Structural modification and biomass digestibility", Renew. Energy, 160, pp.555~563 (2020)
  27. Spiridon I, "Biological and pharmaceutical applications of lignin and its derivatives : a mini-review", Cellulose Chem. Technol, 52, pp.543~550 (2018)
  28. Yan Z, "Effect of different Bacillus strains on the profile of organic acids in a liquid culture of Daqu", J. Inst. Brew, 119, pp.78~83 (2013)
  29. Gil NY, "Metabolite profile and immunomodulatory properties of bellflower root vinegar produced using Acetobacter pasteurianus A11-2", Foods, 9, pp.1~14 (2020)
  30. Dörsam S, "Sustainable carbon sources for microbial organic acid production with filamentous fungi", Biotechnol. Biofuels, 10, pp.242~ (2017)
  31. Avanthi A, "Bioconversion of hemicelluloses of lignocellulosic biomass to ethanol : an attempt to utilize pentose sugars", Biofuels, 8, pp.431~444 (2017)
  32. Dai Z, "Current advance in biological production of malic acid using wild type and metabolic engineered strains", Bioresour. Technol, 258, pp.345~353 (2018)
  33. Zou X, "Biosynthesis of polymalic acid in fermentation : advances and prospects for industrial application", Crit. Rev. Biotechnol, 39, pp.408~421 (2019)
  34. Yegin S, "Valorization of egg shell as a detoxifying and buffering agent for efficient polymalic acid production by Aureobasidium pullulans NRRL Y-2311-1 from barley straw hydrolysate", Bioresour. Technol, 278, pp.130~137 (2019)
  35. Komesu A, "Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass", pp.143~154 (2020), Elsevier
  36. Wei Zeng, "Analysis of the L-malate biosynthesis pathway involved in poly(β-L-malic acid) production in Aureobasidium melanogenum GXZ-6 by addition of metabolic intermediates and inhibitors", The Journal of Microbiology, 57(4), pp.281~287 (2019), 한국미생물학회[DOI]
  37. Xingjiang Li, "High Levels of Malic Acid Production by the Bioconversion of Corn Straw Hydrolyte Using an Isolated Rhizopus Delemar Strain", Biotechnology and Bioprocess Engineering, 19(3), pp.478~492 (2014), 한국생물공학회
  38. Prabhu AA, "Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control", Biotechnol. Biofuels, 13, pp.113~ (2020)
  39. Geyer M, "Malic acid production by Aspergillus oryzae : the role of CaCO3", Chem. Eng, 70, pp.1801~1806 (2018)
  40. Sasaki Y, "Organic acid production by Basidiomycetes. : II. Acid production from xylose", J. Facul. Agr. Hokkaido Univ, 55, pp.174~181 (1967)
  41. Zhang X, "L-malate production by metabolically engineered Escherichia coli", App. Environ. Microbiol, 77, pp.427~434 (2011)
  42. Zeng W, "Development and benefit evaluation of fermentation strategies for poly(malic acid)production from malt syrup by Aureobasidium melanogenum GXZ-6", Bioresour. Technol, 274, pp.479~487 (2019)
  43. Zou X, "Adaptation and transcriptome analysis of Aureobasidium pullulans in corncob hydrolysate for increased inhibitor tolerance to malic acid production", PLoS One, 10, pp.e0121416~ (2015)
  44. Leathers TD, "Production of poly(β-L-malic acid)(PMA)from agricultural biomass substrates by Aureobasidium pullulans", Biotechnol. Lett, 35, pp.83~89 (2013)

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