Skin Barrier-Enhancing Effects of Dermabiotics HDB with Regulation of Skin Microbiota
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In the regulation of inflammatory responses and skin homeostasis, the skin and its microbiota are closely related. Studies have reported that lactic acid bacteria extracts can improve the skin condition and microbiota. In our previous study, we developed probiotic lysates, which are efficacious in improvement of human skin cells and the skin barrier. The skin-moisturizing effect of Dermabiotics HDB (HDB) prepared with Lactiplantibacillus plantarum, and the correlation between changes in the skin microbiota and moisture contents, were evaluated and analyzed in clinical trials. The clinical parameters on the cheeks of 21 female participants were measured using biophysical tools before and after (2 weeks) using HDB or control. The skin microbes were collected and identified using 16s rRNA gene sequencing. HDB significantly improved moisture intensity, transepidermal water loss (TEWL), and hot flush level on the cheek. The beta-diversity of the skin microbiota was different from that of the control in the unweighted UniFrac principal coordinate analysis after using HDB. The genus Lawsonella demonstrated a positive correlation with TEWL and a negative correlation with the moisture contents of the keratin layer, regardless of the use of HDB and control. Conversely, after HDB use, the genus Staphylococcus was increased and associated with a lower hot flush level, while the genera of the phylum Proteobacteria tended to decrease, which is associated with an improved skin condition. Overall, HDB showed clinically proven effects, including skin moisturization with regulation of the skin microbiota.
Abstract
In the regulation of inflammatory responses and skin homeostasis, the skin and its microbiota are closely related. Studies have reported that lactic acid bacteria extracts can improve the skin condition and microbiota. In our previous study, we developed probiotic lysates, which are efficacious in improvement of human skin cells and the skin barrier. The skin-moisturizing effect of Dermabiotics HDB (HDB) prepared with Lactiplantibacillus plantarum, and the correlation between changes in the skin microbiota and moisture contents, were evaluated and analyzed in clinical trials. The clinical parameters on the cheeks of 21 female participants were measured using biophysical tools before and after (2 weeks) using HDB or control. The skin microbes were collected and identified using 16s rRNA gene sequencing. HDB significantly improved moisture intensity, transepidermal water loss (TEWL), and hot flush level on the cheek. The beta-diversity of the skin microbiota was different from that of the control in the unweighted UniFrac principal coordinate analysis after using HDB. The genus Lawsonella demonstrated a positive correlation with TEWL and a negative correlation with the moisture contents of the keratin layer, regardless of the use of HDB and control. Conversely, after HDB use, the genus Staphylococcus was increased and associated with a lower hot flush level, while the genera of the phylum Proteobacteria tended to decrease, which is associated with an improved skin condition. Overall, HDB showed clinically proven effects, including skin moisturization with regulation of the skin microbiota.
참고문헌 (21)
- Oh J, "Biogeography and individuality shape function in the human skin metagenome", Nature, 514, pp.59~64 (2014)
- Grice EA, "The skin microbiome", Nat. Rev. Microbiol, 9, pp.224~253 (2011)
- Yang Y, "Advances in the human skin microflora and its roles in cutaneous diseases", Microb. Cell Fact, 21, pp.176~ (2022)
- Rozas M, "From dysbiosis to healthy skin : major contributions of Cutibacterium acnes to skin homeostasis", Microorganisms, 9, pp.628~ (2021)
- Fournière M, "Staphylococcus epidermidis and Cutibacterium acnes : two major sentinels of skin microflora and the influence of cosmetics", Microorganisms, 8, pp.1752~ (2020)
- Francuzik W, "Propionibacterium acnes abundance correlates inversely with Staphylococcus aureus : data from atopic Ddermatitis skin microbiome", Acta Derm. Venereol, 98, pp.490~495 (2018)
- Skowron K, "Human skin microbiome : impact of intrinsic and extrinsic factors on skin microflora", Microorganisms, 9, pp.543~ (2021)
- Lim HY, "Antiwrinkle and antimelanogenesis effects of tyndallized Lactobacillus acidophilus KCCM12625P", Int. J. Mol. Sci, 21, pp.1620~ (2020)
- Jung YO, "Lysates of a probiotic, Lactobacillus rhamnosus, can improve skin barrier function in a reconstructed human epidermis model", Int. J. Mol. Sci, 20, pp.4289~ (2019)
- Khmaladze I, "Lactobacillus reuteri DSM 17938-A comparative study on the effect of probiotics and lysates on human skin", Exp. Dermatol, 28, pp.822~828 (2019)
- Kim HJ, "Aged related human skin microbiome and mycobiome in Korean women", Sci. Rep, 12, pp.2351~ (2022)
- Yang SJ, "Skin barrier enhancement of ferment using lava seawater and Lactobacillus plantarum HDB1234 as a novel cosmetic ingredient", J. Kor. Soc. Cosmetol, 27, pp.356~363 (2021)
- Parada, AE, "Every base matters : assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples", Environ. Microbiol, 18, pp.1403~1414 (2016)
- Rognes T, "VSEARCH : a versatile open source tool for metagenomics", PeerJ, 4, pp.e2584~ (2016)
- Caporaso JG, "QIIME allows analysis of highthroughput community sequencing data", Nat. Methods, 7, pp.335~ (2010)
- Lee HJ, "Effects of cosmetics on the skin microbiome of facial cheeks with different hydration levels", Microbiology, 7, pp.e00557~ (2018)
- Ogai K, "A comparison of techniques for collecting skin microbiome samples : swabbing versus tape-stripping", Front. Microbiol, 2, pp.2362~ (2018)
- Kim JH, "Taxonomic profiling of skin microbiome and correlation with clinical skin parameters in healthy Koreans", Sci. Rep, 11, pp.16269~ (2021)
- Kim HS, "Microbiota in Rosacea", Am. J. Clin. Dermatol, 21, pp.S25~S35 (2020)
- Callewaert C, "Skin microbiome and its interplay with the environment", Am. J. Clin. Dermatol, 21, pp.4~11 (2020)
- Grice EA, "Topographical and temporal diversity of the human skin microbiome", Science, 324, pp.1190~1192 (2009)