Bacillus siamensis 3BS12-4 Extracellular Compounds as a Potential Biological Control Agent against Aspergillus flavus
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Aspergillus flavus, the primary mold that causes food spoilage, poses significant health and economic problems worldwide. Eliminating A. flavus growth is essential to ensure the safety of agricultural products, and extracellular compounds (ECCs) produced by Bacillus spp. have been demonstrated to inhibit the growth of this pathogen. In this study, we aimed to identify microorganisms efficient at inhibiting A. flavus growth and degrading aflatoxin B1. We isolated microorganisms from soil samples using a culture medium containing coumarin (CM medium) as the sole carbon source. Of the 498 isolates grown on CM medium, only 132 bacterial strains were capable of inhibiting A. flavus growth. Isolate 3BS12-4, identified as Bacillus siamensis, exhibited the highest antifungal activity with an inhibition ratio of 43.10%, and was therefore selected for further studies. The inhibition of A. flavus by isolate 3BS12-4 was predominantly attributed to ECCs, with a minimum inhibitory concentration and minimum fungicidal concentration of 0.512 g/ml. SEM analysis revealed that the ECCs disrupted the mycelium of A. flavus. The hydrolytic enzyme activity of the ECCs was assessed by protease, β-1,3-glucanase, and chitinase activity. Our results demonstrate a remarkable 96.11% aflatoxin B1 degradation mediated by ECCs produced by isolate 3BS12-4. Furthermore, treatment with these compounds resulted in a significant 97.93% inhibition of A. flavus growth on peanut seeds. These findings collectively present B. siamensis 3BS12-4 as a promising tool for developing environmentally friendly products to manage aflatoxin-producing fungi and contribute to the enhancement of agricultural product safety and food security.
Abstract
Aspergillus flavus, the primary mold that causes food spoilage, poses significant health and economic problems worldwide. Eliminating A. flavus growth is essential to ensure the safety of agricultural products, and extracellular compounds (ECCs) produced by Bacillus spp. have been demonstrated to inhibit the growth of this pathogen. In this study, we aimed to identify microorganisms efficient at inhibiting A. flavus growth and degrading aflatoxin B1. We isolated microorganisms from soil samples using a culture medium containing coumarin (CM medium) as the sole carbon source. Of the 498 isolates grown on CM medium, only 132 bacterial strains were capable of inhibiting A. flavus growth. Isolate 3BS12-4, identified as Bacillus siamensis, exhibited the highest antifungal activity with an inhibition ratio of 43.10%, and was therefore selected for further studies. The inhibition of A. flavus by isolate 3BS12-4 was predominantly attributed to ECCs, with a minimum inhibitory concentration and minimum fungicidal concentration of 0.512 g/ml. SEM analysis revealed that the ECCs disrupted the mycelium of A. flavus. The hydrolytic enzyme activity of the ECCs was assessed by protease, β-1,3-glucanase, and chitinase activity. Our results demonstrate a remarkable 96.11% aflatoxin B1 degradation mediated by ECCs produced by isolate 3BS12-4. Furthermore, treatment with these compounds resulted in a significant 97.93% inhibition of A. flavus growth on peanut seeds. These findings collectively present B. siamensis 3BS12-4 as a promising tool for developing environmentally friendly products to manage aflatoxin-producing fungi and contribute to the enhancement of agricultural product safety and food security.
참고문헌 (50)
- Wild CP, "Mycotoxins and human disease : a largely ignored global health issue", Carcinogenesis, 31, pp.71~82 (2010)
- Alpsoy L, "Key roles of vitamins A, C, and E in aflatoxin B1-induced oxidative stress", Vitam. Horm, 86, pp.287~305 (2011)
- Benkerroum N, "Aflatoxins : producing-molds, structure, health issues and Incidence in Southeast Asian and Sub-Saharan African Countries", Int. J. Environ. Res. Public Health, 17, pp.1215~ (2020)
- Verma RJ, "Aflatoxin Cause DNA Damage", International Journal of Human Genetics, 4, pp.231~236 (2004)
- IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, "Some traditional herbal medicines, some mycotoxins, naphthalene and styrene", IARC. Monogr. Eval. Carcinog. Risks. Hum, 82, pp.1~556 (2002)
- Worldwide regulations for mycotoxins in 1995 : a compendium, "Food and Agriculture Organization of the United Nations (FAO)", 1~44, pp.FAO Food and Nutrition Paper 64 (1997)
- Bhatnagar-Mathur P, "Biotechnological advances for combating Aspergillus flavus and aflatoxin contamination in crops", Plant Sci, 234, pp.119~132 (2015)
- Jalili, M., "A review on aflatoxins reduction in food", Iranian J. Health Saf. Environ, 3, pp.445~459 (2016)
- Razzaghi-Abyaneh M, "Inhibitory effects of Satureja hortensis L. essential oil on growth and aflatoxin production by Aspergillus parasiticus", Int. J. Food Microbiol, 123, pp.228~233 (2008)
- Razzaghi-Abyaneh M, "Effects of Heracleum persicum ethyl acetate extract on the growth, hyphal ultrastructure and aflatoxin biosynthesis in Aspergillus parasiticus", Mycotoxin Res, 29, pp.261~269 (2013)
- Pérez-García A, "Plant protection and growth stimulation by microorganisms: biotechnological applications of Bacilli in agriculture", Curr. Opin. Biotechnol, 221, pp.87~93 (2011)
- Oluwafemi F, "Removal of aflatoxins by viable and heat-killed Lactobacillus species isolated from fermented maize", Appl. Biosci, 16, pp.871~876 (2009)
- Guan S, "Aflatoxin B(1)degradation by Stenotrophomonas maltophilia and other microbes selected using coumarin medium", Int. J. Mol. Sci, 9, pp.1489~1503 (2008)
- Suresh G, "Biodegradation of aflatoxin B1 with cell-free extracts of Trametes versicolor and Bacillus subtilis", Res. Vet. Sci, 133, pp.85~91 (2020)
- Bueno D, "Physical adsorption of aflatoxin B1 by lactic acid bacteria and Saccharomyces cerevisiae : a theoretical model", Food Prot, 70, pp.2148~2154 (2007)
- Chaucheyras-Durand F, "Probiotics in animal nutrition and health", Benef. Microbes, 1, pp.3~9 (2010)
- Meena KR, "Lipopeptides as the antifungal and antibacterial agents : applications in food safety and therapeutics", Biomed. Res. Int, 2015, pp.473050~ (2015)
- Baruzzi F, "Science against microbial pathogens. Communicating Current Research and Technological Advances", pp.1102~1111 (2011)
- Shoda M, "Bacterial control of plant diseases", J. Biosci. Bioeng, 89, pp.515~521 (2000)
- Gao X, "Isolation of Bacillus subtilis : screening for aflatoxin B1, M1, and G1 detoxification", Eur. Food Res. Technol, 232, pp.957~962 (2011)
- Hassan M, "Optimization of rhamnolipid production by biodegrading bacterial isolates using Plackett-Burman design", Int. J. Biol. Macromol, 82, pp.573~579 (2016)
- Xia X, "Isolation and characterization of a Bacillus subtilis strain with aflatoxin B1 biodegradation capability", Food Control, 75, pp.92~98 (2017)
- Rao KR, "Biological detoxification of aflatoxin B1 by Bacillus licheniformis CFR1", Food Control, 71, pp.234~241 (2017)
- Arulmozhi P, "Phytochemical analysis and antimicrobial activity of some medicinal plants against selected pathogenic microorganisms", Microb. Pathog, 123, pp.219~226 (2018)
- Ghannoum M, "Activity of TDT 067(terbinafine in Transfersome)against agents of onychomycosis, as determined by minimum inhibitory and fungicidal concentrations", J. Clin. Microbiol, 49, pp.1716~1720 (2011)
- Li B, "Biocontrol potential of 1-pentanal emitted from lactic acid bacteria strains against Aspergillus flavus in red pepper (Capsicum annuum L.)", Food Control, 142, pp.109261~ (2022)
- Tsuchida O, "An alkaline protease of an alkaline Bacillus sp", Curr. Microbiol, 14, pp.7~12 (1986)
- Miller GL, "Use of dinitrosalicylic acid reagent for determination of reducing sugar", Anal. Chem, 31, pp.426~428 (1959)
- Lowry OH, "Protein measurement with the Folin phenol reagent", J. Biol. Chem, 193, pp.265~275 (1951)
- Roman M, "AOAC Single-Laboratory Validation Repeatability Template, personal communication", AOAC Web link pending, ~ (2008)
- Einloft TC, "Biocontrol capabilities of three Bacillus isolates towards aflatoxin B1producer A. flavus in vitro and on maize grains", Food Control, 125, pp.107978~ (2021)
- Shi L, "Ochratoxin A biocontrol and biodegradation by Bacillus subtilis CW 14", J. Sci. Food. Agric, 94, pp.1879~1885 (2014)
- Gong Q, "Identification of bacillomycin D from Bacillus subtilis fmbJ and its inhibition effects against Aspergillus flavus", Food Control, 36, pp.8~14 (2014)
- Chen G, "Detoxification of Aflatoxin B1 by a potential probiotic Bacillus amyloliquefaciens WF2020", Front. Microbiol, 13, pp.891091~ (2022)
- Zhang T, "Antifungal compounds from Bacillus subtilis B-FS06 inhibiting the growth of Aspergillus flavus", J Microbiol Biotechol, 24, pp.783~788 (2008)
- Li S, "Screening of Bacillus velezensis E2 and the inhibitory effect of its antifungal substances on Aspergillus flavus", Foods, 11, pp.140~ (2022)
- Akocak PB, "Antagonistic effect of chitinolytic Pseudomonas and Bacillus on growth of fungal hyphae and spores of aflatoxigenic Aspergillus flavus", Food Biosci, 10, pp.48~58 (2015)
- Thakaew R, "Inhibitory activity of Bacillus subtilis BCC 6327 metabolites against growth of aflatoxigenic fungi isolated from Bird Chili Powder", Int. J. Biosci. Biochem. Bioinform, 3, pp.27~32 (2013)
- Altimira F, "Genomic and experimental analysis of the biostimulant and antagonistic properties of phytopathogens of Bacillus safensis and Bacillus siamensis", Microorganisms, 10, pp.670~ (2022)
- Peberdy JF, "A Review in Biochemistry of Cell Walls and Membranes in Fungi", pp.5~30 (1990), Springer-Verlag
- Mahmoud ALE, "Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential", BMC Microbiol, 23, pp.269~ (2023)
- Schönbichler A, "Exploring the potential for fungal antagonism and cell wall attack by Bacillus subtilis natto", Front. Microbiol, 11, pp.521~ (2020)
- Pozo MJ, "β-1, 3-Glucanase activities in tomato roots inoculated with arbuscular mycorrhizal fungi and", or Phytophthora parasitica and their possible involvement in bioprotection, 1999, pp.141, 149~157
- Cho J-I, "Control of aflatoxin production of Aspergillus flavus by inhibitory action of antagonistic bacteria", J. Microbiol. Biotechnol, 10, pp.154~160 (2000)
- Shakeel Q, "Biocontrol of Aspergillus flavus on peanut Kernels using Streptomyces yanglinensis 3-10", Front. Microbiol, 9, pp.1049~ (2018)
- Bluma RV, "Influence of Bacillus spp. isolated from maize agroecosystem on growth and aflatoxin B (1) production by Aspergillus section Flavi", Pest. Manag. Sci, 62, pp.242~251
- Liu Y, "Antimicrobial Bacillus velezensis HC6 : production of three kinds of lipopeptides and biocontrol potential in maize", J. Appl. Microbiol, 128, pp.242~254 (2020)
- Mendu L, "Seed coat mediated resistance against Aspergillus flavus infection in peanut", Plant Gene, 32, pp.100381~ (2022)
- Parte AC, "List of prokaryotic names with standing in nomenclature(LPSN)moves to the DSMZ", Int. J. Syst. Evol. Microbiol, 70, pp.5607~5612 (2020)
- Heo S, "Functional genomic insights into probiotic Bacillus siamensis strain B28 from traditional Korean fermented kimchi", Foods, 10, pp.1906~ (2021)
