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Functional Characterization of khadi Yeasts Isolates for Selection of Starter Cultures

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Yeasts play an important role in spontaneous fermentation of traditional alcoholic beverages. Our previous study revealed that a mixed-consortia of both Saccharomyces and non-Saccharomyces yeasts were responsible for fermentation of khadi, a popular, non-standardized traditional beverage with an immense potential for commercialization in Botswana. Functional characterization of isolated fermenting yeasts from mixed consortia is an indispensable step towards the selection of potential starter cultures for commercialization of khadi. In this study, we report the characterization of 13 khadi isolates for the presence of brewing-relevant phenotypes such as their fermentative capacity, ability to utilize a range of carbon sources and their ability to withstand brewing-associated stresses, as a principal step towards selection of starter cultures. Khadi isolates such as Saccharomyces cerevisiae, Saccharomycodes ludwigii and Candida ethanolica showed good brewing credentials but Lachancea fermentati emerged as the isolate with the best brewing attributes with a potential as a starter culture. However, we were then prompted to investigate the potential of L. fermentati to influence the fruity aromatic flavor, characteristic of khadi. The aroma components of 18 khadi samples were extracted using headspace solid phase micro-extraction (HSSPME) and identified using a GC-MS. We detected esters as the majority of volatile compounds in khadi, typical of the aromatic signature of both khadi and L. fermentati associated fermentations. This work shows that L. fermentati has potential for commercial production of khadi.

Abstract

Yeasts play an important role in spontaneous fermentation of traditional alcoholic beverages. Our previous study revealed that a mixed-consortia of both Saccharomyces and non-Saccharomyces yeasts were responsible for fermentation of khadi, a popular, non-standardized traditional beverage with an immense potential for commercialization in Botswana. Functional characterization of isolated fermenting yeasts from mixed consortia is an indispensable step towards the selection of potential starter cultures for commercialization of khadi. In this study, we report the characterization of 13 khadi isolates for the presence of brewing-relevant phenotypes such as their fermentative capacity, ability to utilize a range of carbon sources and their ability to withstand brewing-associated stresses, as a principal step towards selection of starter cultures. Khadi isolates such as Saccharomyces cerevisiae, Saccharomycodes ludwigii and Candida ethanolica showed good brewing credentials but Lachancea fermentati emerged as the isolate with the best brewing attributes with a potential as a starter culture. However, we were then prompted to investigate the potential of L. fermentati to influence the fruity aromatic flavor, characteristic of khadi. The aroma components of 18 khadi samples were extracted using headspace solid phase micro-extraction (HSSPME) and identified using a GC-MS. We detected esters as the majority of volatile compounds in khadi, typical of the aromatic signature of both khadi and L. fermentati associated fermentations. This work shows that L. fermentati has potential for commercial production of khadi.

참고문헌 (35)

  1. Motlhanka K, "Microbial and chemical diversity of traditional non-cereal based alcoholic beverages of Sub-Saharan Africa", Beverages, 4, pp.1~25 (2018)
  2. Mapitse R, "Analysis of volatile compounds in khadi(an unrecorded alcohol beverage)from botswana by gas chromatography-flame ionization detection(GC-FID)", S. Afr. J. Chem, 67, pp.184~188 (2014)
  3. RudloffA, "Producers, Sellers, and Drinkers: Studies of Noncommercial Alcohol in Nine Countries", pp.13~18 (2012), Glob. Actions Harmful Drink
  4. Nerve Zhou, "Yeast–bacteria competition induced new metabolic traits through large-scale genomic rearrangements in Lachancea kluyveri", FEMS Yeast Research, 17(6) (2017), Oxford University Press (OUP)[DOI]
  5. Steensels J, "Taming wild yeast : potential of conventional and nonconventional yeasts in industrial fermentations", Annu. Rev. Microbiol, 68, pp.61~80 (2014)
  6. Motlhanka K, "Fermentative microbes of khadi, a traditional alcoholic beverage of botswana", Fermentation, 6, pp.51~ (2020)
  7. Zhou N, "Kazachstania gamospora and Wickerhamomyces subpelliculosus : Two alternative baker’s yeasts in the modern bakery", Int. J. Food Microbiol, 250, pp.45~58 (2017)
  8. Liu XZ, "Towards an integrated phylogenetic classification of the Tremellomycetes", Stud. Mycol, 81, pp.85~147 (2015)
  9. Kevin J. Verstrepen, "The Development of Superior Yeast Strains for the Food and Beverage Industries: Challenges, Opportunities and Potential Benefits", Yeasts in Food and Beverages, pp.399~444 (2006), Springer Berlin Heidelberg[DOI]
  10. Hittinger CT, "Diverse yeasts for diverse fermented beverages and foods", Curr. Opin. Biotechnol, 49, pp.199~206 (2018)
  11. Donalies UEB, "Improvement of Saccharomyces yeast strains used in brewing, wine making and baking", Adv. Biochem. Eng. Biotechnol, 111, pp.67~68 (2008)
  12. Johnson EA, "Yeast Biotechnology" (2011), Elsevier B.V.
  13. Hallsworth JE, "Ethanol-induced water stress and fungal growth", J. Ferment. Bioeng, 86, pp.451~456 (1998)
  14. Stanley D, "The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae", J. Appl. Microbiol, 109, pp.13~24 (2010)
  15. E.M. Yahia, "Date ( Phoenix dactylifera L.)", Postharvest Biology and Technology of Tropical and Subtropical Fruits, pp.41~81e (2011), Elsevier[DOI]
  16. Génard M, "Changes in fruit sugar concentrations in response to assimilate supply, metabolism and dilution : a modeling approach applied to peach fruit(Prunus persica)", Tree Physiol, 23, pp.373~385 (2003)
  17. Menezes AGT, "Combination of probiotic yeast and lactic acid bacteria as starter culture to produce maize-based beverages", Food Res. Int, 111, pp.187~197 (2018)
  18. Abiola C, "Wine fermentation", Data Br, 2, pp.73~77 (2014)
  19. Matsushita K, "Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations", Biosci. Biotechnol. Biochem, 80, pp.655~668 (2016)
  20. Li SS, "Yeast species associated with wine grapes in China", Int. J. Food Microbiol, 138, pp.85~90 (2010)
  21. Nova MXV, "Yeast species involved in artisanal cachaça fermentation in three stills with different technological levels in Pernambuco, Brazil", Food Microbiol, 26, pp.460~466 (2009)
  22. Gulitz A, "The microbial diversity of water kefir", Int. J. Food Microbiol, 151, pp.284~288 (2011)
  23. Maciel NOP, "The diversity and antifungal susceptibility of the yeasts isolated from coconut water and reconstituted fruit juices in Brazil", Int. J. Food Microbiol, 160, pp.201~205 (2013)
  24. Romano P, "Secondary products formation as a tool for discriminating non-Saccharomyces wine strains strain diversity in non-Saccharomyces wine yeasts", Antonie Van Leeuwenhoek, 77, pp.239~242 (1997)
  25. Cordero-Bueso G, "Biotechnological potential of non-Saccharomyces yeasts isolated during biotechnological potential of non- Saccharomyces yeasts isolated during spontaneous fermentations of Malvar ( Vitis vinifera cv . L .)", Eur. Food Res. Technol, 236, pp.2564~2568 (2012)
  26. Porter TJ, "Biochemical characterization and evaluation of the oenological attributes of Lachancea species" (2017), Stellenbosch University
  27. Angela Capece, "Conventional and Non-Conventional Yeasts in Beer Production", Fermentation, 4(2), pp.38~ (2018), MDPI AG[DOI]
  28. De Roos J, "Microbial acidification, alcoholization, and aroma production during spontaneous lambic beer production", J. Sci. Food Agric, 99, pp.25~38 (2019)
  29. Holt S, "Bioflavoring by non-conventional yeasts in sequential beer fermentations", Food Microbiol, 72, pp.55~66 (2018)
  30. Querol A, "Yeasts in Food and Beverages" (2011)
  31. Vejarano R, "Saccharomycodes ludwigii, control and potential uses in winemaking processes", Fermentation, 4, pp.1~19 (2018)
  32. Gutiérrez A, "Evaluation of non-Saccharomyces yeasts in the fermentation of wine, beer and cider for the development of new beverages", J. Inst. Brew, 124, pp.389~402 (2018)
  33. Karamoko D, "The biochemical and microbiological quality of palm wine samples produced at different periods during tapping and changes which occured during their storage", Food Control, 26, pp.504~511 (2012)
  34. Loira I, "Use of Schizosaccharomyces pombe and Torulaspora delbrueckii strains in mixed and sequential fermentations to improve red wine sensory quality", FRIN, 76, pp.325~333 (2015)
  35. Garofalo C, "Selection of indigenous yeast strains for the production of sparkling wines from native Apulian grape varieties", Int. J. Food Microbiol, 285, pp.7~17 (2018)

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