Isolation and Characterization of Potential Starter Yeasts from Traditional Moroccan Sourdoughs
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The increasing demand for baked products has given a boost to research on isolation and selection of novel yeast strains with improved leavening activity. Twelve sourdough samples were collected from several localities of the Fez region in Morocco. The pH and total titratable acidity (TTA) values of these samples varied from 3.03-4.63 and 14-17.5 ml of 0.1 N NaOH/10 g of sourdough, respectively, while yeast counts ranged from 5.3 6.77 Log CFU/g. Thirty-two yeast isolates were obtained and evaluated for their leavening ability. Out of all isolates, four yeasts molecularly identified as Saccharomyces cerevisiae (three strains) and Kluyveromyces marxianus (one strain) showed highest specific volumes of 4.69, 4.55, 4.35 and 4.1 cm3/g, respectively. These strains were further assessed for their tolerance to high concentrations of salt, sugar, elevated temperatures, and low pH conditions. K. marxianus showed higher resistance than the S. cerevisiae. Thus, Moroccan sourdoughs harbor technologically relevant yeasts that could be used as potential starters for bread preparation.
Abstract
The increasing demand for baked products has given a boost to research on isolation and selection of novel yeast strains with improved leavening activity. Twelve sourdough samples were collected from several localities of the Fez region in Morocco. The pH and total titratable acidity (TTA) values of these samples varied from 3.03-4.63 and 14-17.5 ml of 0.1 N NaOH/10 g of sourdough, respectively, while yeast counts ranged from 5.3 6.77 Log CFU/g. Thirty-two yeast isolates were obtained and evaluated for their leavening ability. Out of all isolates, four yeasts molecularly identified as Saccharomyces cerevisiae (three strains) and Kluyveromyces marxianus (one strain) showed highest specific volumes of 4.69, 4.55, 4.35 and 4.1 cm3/g, respectively. These strains were further assessed for their tolerance to high concentrations of salt, sugar, elevated temperatures, and low pH conditions. K. marxianus showed higher resistance than the S. cerevisiae. Thus, Moroccan sourdoughs harbor technologically relevant yeasts that could be used as potential starters for bread preparation.
참고문헌 (56)
- Newberry MP, "Dynamic and elongation rheology of yeasted bread doughs", Cereal Chem, 79, pp.874~879 (2002)
- Liao Y, "Role of hydrogen peroxide produced by baker’s yeast on dough rheology", Cereal Chem, 75, pp.612~616 (1998)
- Romano A, "Description of leavening of bread dough with mathematical modelling", J. Food Eng, 83, pp.142~148 (2007)
- Poitrenaud B, "Handbook of food and beverage fermentation technology", pp.695~719 (2004), Marcel Dekker
- Gélinas P, "In search of perfect growth media for Baker’s yeast production: Mapping patents", Compr. Rev. Food Sci. Food Saf, 11, pp.13~33 (2012)
- Phaff HJ, "The life of yeasts" (2013), Harvard University Press
- Dequin S, "The potential of genetic engineering for improving brewing, wine-making and baking yeasts", Appl. Microbiol. Biotechnol, 56, pp.577~588 (2001)
- Ostergaard S, "Metabolic engineering of Saccharomyces cerevisiae", Microbiol. Mol. Biol. Rev, 64, pp.34~50 (2000)
- Li H, "Bacterial diversity in traditional Jiaozi and sourdough revealed by high-throughput sequencing of 16S rRNA amplicons", LWT-Food Sci. Technol, 81, pp.319~325 (2017)
- Gänzle M, "Composition and function of sourdough microbiota: From ecological theory to bread quality", Int. J. Food Microbiol, 239, pp.19~25 (2016)
- De Vuyst L, "Yeast diversity of sourdoughs and associated metabolic properties and functionalities", Int. J. Food Microbiol, 239, pp.26~34 (2016)
- Minervini F, "Lactic acid bacterium and yeast microbiotas of 19 sourdoughs used for traditional", typical Italian breads:interactions between ingredients and microbial species diversity, 2012, pp.78, 1251~1264
- Alfonzo A, "Development of a method for the direct fermentation of semolina by selected sourdough lactic acid bacteria", Int. J. Food Microbiol, 239, pp.65~78 (2016)
- Gobbetti M, "Drivers for the establishment and composition of the sourdough lactic acid bacteria biota", Int. J. Food Microbiol, 239, pp.3~18 (2016)
- Gänzle M, "Handbook on Sourdough Biotechnology", pp.183~216 (2013), Springer US
- Huys G, "Handbook on Sourdough Biotechnology", pp.105~154 (2013), Springer US
- Perricone M, "Technological characterization and probiotic traits of yeasts isolated from Altamura sourdough to select promising microorganisms as functional starter cultures for cereal-based products", Food Microbiol, 38, pp.26~35 (2014)
- Katina K, "Effects of sourdough and enzymes on staling of high-fibre wheat bread", LWT-Food Sci. Technol, 39, pp.479~491 (2006)
- Kariluoto S, "In situ enrichment of folate by microorganisms in beta-glucan rich oat and barley matrices", Int. J. Food Microbiol, 176, pp.38~48 (2014)
- Türk M, "Inositol hexaphosphate hydrolysis by Baker’s yeast. Capacity, kinetics, and degradation products", J. Agric. Food Chem, 48, pp.100~104 (2000)
- Moslehi-Jenabian S, "Beneficial effects of probiotic and food borne yeasts on human health", Nutrients, 2, pp.449~473 (2010)
- Zhou N, "Kazachstania gamospora and Wickerhamomyces subpelliculosus: Two alternative baker’s yeasts in the modern bakery", Int. J. Food Microbiol, 250, pp.45~58 (2017)
- Aslankoohi E, "Non-conventional yeast strains increase the aroma complexity of bread", PLoS One, 11, pp.e0165126~ (2016)
- Pacheco A, "Struct. Funct. Food Eng. Rij", pp.339~370 (2012), InTech
- Almeida MJ, "Leavening ability and freeze tolerance of yeasts isolated from traditional corn and rye bread doughs", Appl. Environ. Microbiol, 62, pp.4401~4404 (1996)
- AACC, "Approved methods of the AACC. Titratable Acidity. Method 02-31.01. 11th Ed", American Association of Cereal Chemists, ~
- Harju S, "Rapid isolation of yeast genomic DNA: Bust n’Grab", BMC Biotechnol, 4, pp.1~6 (2004)
- Lhomme E, "Lactic acid bacterium and yeast microbiotas of sixteen French traditional sourdoughs", Int. J. Food Microbiol, 215, pp.161~170 (2015)
- Arendt EK, "Impact of sourdough on the texture of bread", Food Microbiol, 24, pp.165~174 (2007)
- Palla M, "Exploitation of autochthonous Tuscan sourdough yeasts as potential starters", Int. J. Food Microbiol, 302, pp.59~68 (2019)
- Hammes WP, "Microbial ecology of cereal fermentations", Trends Food Sci. Technol, 16, pp.4~11 (2005)
- Asyikeen ZN, "A new source of Saccharomyces cerevisiae as a leavening agent in bread making", Int. Food Res. J, 20, pp.967~ (2013)
- Caballero R, "Evaluation of Kluyveromyces marxianus as baker’s yeast", Food Res. Int, 28, pp.37~41 (1995)
- Karim A, "Kluyveromyces marxianus: An emerging yeast cell factory for applications in food and biotechnology", Int. J. Food Microbiol, 333, pp.108818~ (2020)
- Bonekamp FJ, "On the safety of Kluyveromyces lactis—a review", Appl. Microbiol. Biotechnol, 41, pp.1~3 (1994)
- Nikolaou E, "Selection of indigenous Saccharomyces cerevisiae strains according to their oenological characteristics and vinification results", Food Microbiol, 23, pp.205~211 (2006)
- Joosten H, "Detection, growth, and amineproducing capacity of lactobacilli in cheese", Appl. Environ. Microbiol, 55, pp.2356~2359 (1989)
- Oda Y, "Hybridization of bakers’ yeast by the raremating method to improve leavening ability in dough", Enzyme Microb. Technol, 12, pp.989~993 (1990)
- Randez-Gil F, "Genetic and phenotypic characteristics of baker’s yeast: relevance to baking", Annu. Rev. Food Sci. Technol, 4, pp.191~214 (2013)
- Donalies UE, "Improvement of Saccharomyces yeast strains used in brewing, wine making and baking", Adv. Biochem. Eng. Biotechnol, 111, pp.67~98 (2008)
- Vaisey M, "Flour composition, chemical constituents of flour from cytologically synthesized and natural cereal species", J. Agric. Food Chem, 12, pp.84~86 (1964)
- Kurtzman CP, "Advances in yeast systematics and phylogeny and their use as predictors of biotechnologically important metabolic pathways", FEMS Yeast Res, 15, pp.fov050~ (2015)
- Noort MW, "Saltiness enhancement in bread by inhomogeneous spatial distribution of sodium chloride", J. Cereal Sci, 52, pp.378~386 (2010)
- Quílez J, "Relationships between sensory flavor evaluation and volatile and nonvolatile compounds in commercial wheat bread type baguette", J. Food Sci, 71, pp.S423~S427 (2006)
- Takagi H, "Stress Biology of Yeasts and Fungi: Applications for Industrial Brewing and Fermentation", pp.23~42 (2015), Springer Japan
- Struyf N, "Bread dough and baker’s yeast: An uplifting synergy", Compr. Rev. Food Sci. Food Saf, 16, pp.850~867 (2017)
- Zhang J, "Optimization and microbial community analysis of anaerobic co-digestion of food waste and sewage sludge based on microwave pretreatment", Bioresour. Technol, 200, pp.253~261 (2016)
- Verstrepen KJ, "Glucose and sucrose: hazardous fastfood for industrial yeast?", Trends Biotechnol, 22, pp.531~537 (2004)
- Saini P, "Physiological response of Kluyveromyces marxianus during oxidative and osmotic stress", Process Biochem, 56, pp.21~29 (2017)
- Aldiguier AS, "Synergistic temperature and ethanol effect on Saccharomyces cerevisiae dynamic behaviour in ethanol bio-fuel production", Bioprocess Biosyst. Eng, 26, pp.217~222 (2004)
- Torija MJ, "Effects of fermentation temperature on the strain population of Saccharomyces cerevisiae", Int. J. Food Microbiol, 80, pp.47~53 (2003)
- Fonseca GG, "The yeast Kluyveromyces marxianus and its biotechnological potential", Appl. Microbiol. Biotechnol, 79, pp.339~354 (2008)
- Maaruf A, "Leavening ability of yeast isolated from different local fruits in bakery product", Sains Malays, 40, pp.1413~1419 (2011)
- Reale A, "Stabilization of sourdough starter by spray drying technique : New breadmaking perspective", LWT, 99, pp.468~475 (2019)
- De Vuyst L, "The sourdough microflora: biodiversity and metabolic interactions", Trends Food Sci. Technol, 16, pp.43~56 (2005)
- Catzeddu P, "Flour and Breads and their Fortification in Health and Disease Prevention", pp.177~188 (2019), Academic Press
