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Microbial Strains and Bioactive Exopolysaccharide Producers from Thai Water Kefir

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초록

The aims of this novel work were to determine the microbial strains and exopolysaccharide (EPS) producers in water kefir from Nakhon Ratchasima Province, Thailand. Thirty-three microbial strains were identified using 16S rRNA gene analysis consisting of 18 bacterial strains, as 9 strains of acetic acid bacteria (AAB), 9 strains of lactic acid bacteria (LAB), and 15 yeast strains. All bacteria were able to produce EPS with a diverse appearance on agar media containing different sugars at a concentration of 8%. Culture supernatants from AAB and LAB showed 31−64% 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity with the highest antioxidant activity of 64% from Acetobacter pasteurianus WS3 and WS6. Crude EPS from A. pasteurianus WS3 displayed the highest ferric reducing antioxidant power at 280 mM FeSO4/g EPS, greatest anti-tyrosinase activity at 20.35%, and highest EPS production of 1,505 mg EPS/L from 8% sucrose. These microbes offer beneficial health implications and their EPSs can be used as food additives and cosmetic ingredients.

Abstract

The aims of this novel work were to determine the microbial strains and exopolysaccharide (EPS) producers in water kefir from Nakhon Ratchasima Province, Thailand. Thirty-three microbial strains were identified using 16S rRNA gene analysis consisting of 18 bacterial strains, as 9 strains of acetic acid bacteria (AAB), 9 strains of lactic acid bacteria (LAB), and 15 yeast strains. All bacteria were able to produce EPS with a diverse appearance on agar media containing different sugars at a concentration of 8%. Culture supernatants from AAB and LAB showed 31−64% 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity with the highest antioxidant activity of 64% from Acetobacter pasteurianus WS3 and WS6. Crude EPS from A. pasteurianus WS3 displayed the highest ferric reducing antioxidant power at 280 mM FeSO4/g EPS, greatest anti-tyrosinase activity at 20.35%, and highest EPS production of 1,505 mg EPS/L from 8% sucrose. These microbes offer beneficial health implications and their EPSs can be used as food additives and cosmetic ingredients.

참고문헌 (47)

  1. Nielsen B, "Kefir: a multifaceted fermented dairy product", Probiotics Antimicrob. Proteins, 6, pp.123~135 (2014)
  2. John SM, "Properties and benefits of kefir -A review", Songklanakarin J. Sci. Technol, 37, pp.275~282 (2015)
  3. Diosma G, "Yeasts from kefir grains: isolation, identification, and probiotic characterization", World J. Microbiol. Biotechnol, 30, pp.43~53 (2014)
  4. Miguel MG, "Identification and assessment of kefir potential for sugar", ethanol-resistance, 2013, pp.44, 113~118
  5. Silva KR, "Antimicrobial activity of broth fermented with kefir grains", Appl. Microbiol. Biotechnol, 152, pp.152~316 (2009)
  6. Waldherr FW, "Identification and characterization of a glucan-producing enzyme from Lactobacillus hilgardii TMW 1.828 involved in granule formation of water kefir", Food Microbiol, 27, pp.672~678 (2010)
  7. Mitsue T, "Efficient kefiran production by a mixed culture of Lactobacillus kefiranofaciens KF-75 and yeast strains", J. Biosci. Bioeng, 87, pp.400~ (1999)
  8. Deeraksa A, "Characterization and spontaneous mutation of a novel gene, polE, involved in pellicle formation in Acetobacter tropicalis SKU1100", Microbiology, 151, pp.4111~4120 (2005)
  9. Freitas F, "Emulsifying behaviour and rheological properties of the extracellular polysaccharide produced by Pseudomonas oleovorans grown on glycerol by product", Carbohydr. Polym, 78, pp.549~556 (2009)
  10. Mahapatra S, "Fungal Exopolysaccharide: production, composition and applications", Microbiol. Insights, 6, pp.1~16 (2013)
  11. Parikh A, "Partial characterization of extracellular polysaccharides from Cyanobacteria", Bioresour. Technol, 97, pp.1822~1827 (2006)
  12. Sutherland IW., "Microbial polysaccharides from gram-negative bacteria", Int. Dairy J, 11, pp.663~674 (2001)
  13. Mota R, "Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110", Carbohydr. Polym, 92, pp.1408~1415 (2013)
  14. Luang-In V, "Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities", LWTFood Sci. Technol, 73, pp.592~601 (2016)
  15. Kimura M., "A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences", J. Mol. Evol, 16, pp.111~120 (1980)
  16. Kumar S, "MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets", Mol. Biol. Evol, 33, pp.1870~1874 (2016)
  17. "FigTree v1. 4. Molecular evolution, phylogenetics and epidemiology" (2012)[URL]
  18. Paulo EM, "An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation", Ciência e Tecnologia de Alimentos, 32, pp.710~714 (2012)
  19. Benzie IFF, "The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power : The FRAP Assay", Anal. Biochem, 239, pp.70~76 (1996)
  20. Seeram NP, "Blackberry, black raspberry, blueberry, cranberry, red rasp berry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro", J. Agric. Food Chem, 54, pp.9329~9339 (2006)
  21. Choi HK, "Free-radicalscavenging and tyrosinase-inhibition activities of Cheonggukjang samples fermented for various times", Food Chem, 106, pp.564~568 (2008)
  22. Li L, "Acetobacter sicerae sp. nov., isolated from cider and kefir, and identification of strains of the genus Acetobacter by dnaK, groEL and rpoB sequence analysis", Int. J. Syst. Evol. Microbiol, 64, pp.2407~2415 (2014)
  23. Chen Z, "Isolation of exopolysaccharide-producing bacteria and yeasts from Tibetan kefir and characterisation of the exopolysaccharides", Int. J. Dairy Technol, 69, pp.410~417 (2016)
  24. Wang Y, "Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet", Appl. Microbiol. Biotech, 84, pp.341~347 (2009)
  25. Rao BP, "Characterization of exopolysaccharide from Bacillus amyloliquefaciens BPRGS for its bioflocculant activity", Int. J. Sci. Eng. Res, 4, pp.1696~1704 (2013)
  26. Coda R, "Antifungal activity of Meyerozyma guilliermondii: identification of activity compounds synthesized during dough fermentation and their effect on long-term storage of wheat bread", Food Microbiol, 33, pp.243~251 (2013)
  27. Osimani A, "Unpasteurised commercial boza as a source of microbial diversity", Int. J. Food Microbiol, 194, pp.62~70 (2015)
  28. Gulitz A, "The microbial diversity of water kefir", Int. J. Food Microbiol, 151, pp.284~288 (2011)
  29. Gruter M, "Structure of the exopolysaccharide produced by Lactococcus lactis sub cremoris H414 grown in a defined medium or skimmed-milk", Carbohydr. Res, 231, pp.273~291 (1992)
  30. van Casteren WHM, "Structural characterisation and enzymatic modification of the exopolysaccharide produced by Lactococcus lactis subsp. cremoris B891", Carbohydr. Res, 327, pp.411~422 (2000)
  31. Ali IAI, "Structural characterization of pellicle polysaccharides of Acetobacter tropicalis SKU1100 wild type and mutant strains", Carbohydr. Polym, 86, pp.1000~1006 (2011)
  32. Grobben GJ, "Influence of fructose and glucose on the production of exopolysaccharides and the activities of enzymes involved in the sugar metabolism and the synthesis of sugar nucleotides in Lactobacillus delbrueckii subsp. Bulgaricus NCFB 2772", Appl. Microbiol. Biotechnol, 46, pp.279~284 (1996)
  33. Looijesteijn PJ, "Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source", Appl. Environ. Microbiol, 65, pp.5003~5008 (1999)
  34. Zhang T, "Growth and exopolysaccharide production by Streptococcus thermophilus ST1 in skim milk", Braz. J. Microbiol, 42, pp.1470~1478 (2011)
  35. Yang JH, "Antioxidant properties of fermented soybean broth", Food Chem, 71, pp.249~254 (2000)
  36. Sakanaka S, "Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha)", Food Chem, 89, pp.569~675 (2005)
  37. Moonmangmee S, "Purification and characterization of a novel polysaccharide involved in the pellicle produced by a thermotolerant Acetobacter strain", Biosci. Biotechnol. Biochem, 66, pp.777~783 (2002)
  38. Li N, "Improvement of exopolysaccharide production in Lactobacillus casei LC2W by overexpression of NADH oxidase gene", Microbiol. Res, 171, pp.73~77 (2015)
  39. Cerning J, "Carbon source requirements for exopolysaccharide production by Lactobacillus casei CG11 and partial structure analysis of the polymer", Appl. Environ. Microbiol, 60, pp.3914~3919 (1994)
  40. Mozzi F, "Exopolysaccharide production by Lactobacillus casei in milk under different growth conditions", Milchwissenschaft, 51, pp.670~673 (1996)
  41. Bajpai VK, "Partially purified exo-polysaccharide from Lactobacillus sakei Probio 65 with antioxidant, α-glucosidase and tyrosinase inhibitory potential", J. Food Biochem, 40, pp.264~274 (2016)
  42. Heung Soo Baek, "The Inhibitory Effect of New Hydroxamic Acid Derivatives on Melanogenesis", Bulletin of the Korean Chemical Society, 29(1), pp.43~46 (2008), 대한화학회
  43. Hima Bindu NSVSSSL, "Biological activities of exopolysaccharide from the mushroom", Fomitopsis feei. Int. J. Microbiol. Res, 8, pp.48~58 (2017)
  44. Song YR, "Exopolysaccharide produced by Bacillus licheniformis strains isolated from Kimchi", J. Gen. Appl. Microbiol, 57, pp.169~175 (2011)
  45. Liu J, "In vitro and in vivo antioxidant activity of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3", Carbohydr. Polym, 82, pp.1278~1283 (2010)
  46. Ahmed Z, "Characterization of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir-Part II", Food Hydrocoll, 30, pp.343~350 (2013)
  47. Vijitra Luang-In, "Characterization and Bioactivities of a Novel Exopolysaccharide Produced from Lactose by Bacillus tequilensis PS21 Isolated from Thai Milk Kefir", 한국미생물·생명공학회지, 46(1), pp.9~17 (2018), 한국미생물·생명공학회

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