Antimicrobial Activity of Cold Atmospheric Plasma on Bacterial Strains Derived from Patients with Diabetic Foot Ulcers
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Bacterial infections or their biofilms in diabetic foot ulcer (DFU) are a key cause of drug-resistant wounds and amputations. Cold atmospheric plasma (CAP) is well documented for its antibacterial effect and promoting wound healing. In the current study, we built an argon- based, custom CAP device and investigated its potential in eliminating laboratory and clinical bacterial strains derived from DFU. The CAP device performed as expected with generation of hydroxyl, reactive nitrogen species, and argon species as determined by optical emission spectroscopy. A dose-dependent increase in oxidation reduction potential (ORP) and nitrites in the liquid phase was observed. The CAP treatment eliminated both gram-positive (Staphylococcus aureus, Entrococcus faecalis) and negative bacteria (Pseudomonas aeruginosa, Proteus mirabilis) laboratory strains. Clinical samples collected from DFU patients exhibited a significant decrease in both types of bacteria, with grampositive strains showing higher susceptibility to the CAP treatment in an ex vivo setting. Moreover, exposure to CAP of polymicrobial biofilms from DFU led to a notable disruption in biofilm and an increase in free bacterial DNA. The duration of CAP exposure used in the current study did not induce DNA damage in peripheral blood lymphocytes. These results suggest that CAP could serve as an excellent tool in treating patients with DFUs.
Abstract
Bacterial infections or their biofilms in diabetic foot ulcer (DFU) are a key cause of drug-resistant wounds and amputations. Cold atmospheric plasma (CAP) is well documented for its antibacterial effect and promoting wound healing. In the current study, we built an argon- based, custom CAP device and investigated its potential in eliminating laboratory and clinical bacterial strains derived from DFU. The CAP device performed as expected with generation of hydroxyl, reactive nitrogen species, and argon species as determined by optical emission spectroscopy. A dose-dependent increase in oxidation reduction potential (ORP) and nitrites in the liquid phase was observed. The CAP treatment eliminated both gram-positive (Staphylococcus aureus, Entrococcus faecalis) and negative bacteria (Pseudomonas aeruginosa, Proteus mirabilis) laboratory strains. Clinical samples collected from DFU patients exhibited a significant decrease in both types of bacteria, with grampositive strains showing higher susceptibility to the CAP treatment in an ex vivo setting. Moreover, exposure to CAP of polymicrobial biofilms from DFU led to a notable disruption in biofilm and an increase in free bacterial DNA. The duration of CAP exposure used in the current study did not induce DNA damage in peripheral blood lymphocytes. These results suggest that CAP could serve as an excellent tool in treating patients with DFUs.
참고문헌 (40)
- Tabares FL, "Cold plasma systems and their application in surface treatments for medicine", Molecules, 26, pp.1903~ (2021)
- Veda Prakash G, "Expansion dynamics of atmospheric pressure helium plasma jet in ambient air", Phys. Lett, 384, pp.126497~ (2020)
- Veda Prakash G, "Concise characterization of cold atmospheric pressure helium plasma jet", AIP Adv, 11, pp.085239~ (2021)
- M L, "Mechanisms of interaction of cold plasma with bacteria", AIP Conference Proceedings, 1041, pp.87~90 (2008)
- Mravlje J, "Development of cold plasma technologies for surface decontamination of seed fungal pathogens : Present status and perspectives", J. Fungi(Basel), 7, pp.650~ (2021)
- Brany D, "Cold atmospheric plasma : A powerful tool for modern medicine", Int. J. Mol. Sci, 21, pp.2923~ (2020)
- T VONW, "Plasma medicine : A field of applied redox biology", In Vivo, 33, pp.1011~1026 (2019)
- Banday MZ, "Pathophysiology of diabetes : An overview", Avicenna J. Med, 10, pp.174~188 (2020)
- Armstrong DG, "Diabetic foot ulcers : A review", JAMA, 330, pp.62~75 (2023)
- Bowler PG, "Wound microbiology and associated approaches to wound management", Clin. Microbiol. Rev, 14, pp.244~269 (2001)
- Sadeghpour Heravi F, "Bacterial diversity of diabetic foot ulcers : Current status and future prospectives", J. Clin. Med, 8, pp.1935~ (2019)
- Chai W, "The profile of microbiological pathogens in diabetic foot ulcers", Front. Med(Lausanne), 8, pp.656467~ (2021)
- Kale DS, "Diabetic foot ulcer in India : Aetiological trends and bacterial diversity", Ind. J. Endocrinol. Metab, 27, pp.107~114 (2023)
- Banu A, "Spectrum of bacteria associated with diabetic foot ulcer and biofilm formation : A prospective study", Australas Med. J, 8, pp.280~285 (2015)
- Macdonald KE, "The microbiology of diabetic foot infections : a meta-analysis", BMC Infect. Dis, 21, pp.770~ (2021)
- Anju VT, "Polymicrobial infections and biofilms : Clinical significance and eradication strategies", Antibiotics(Basel), 11, pp.1731~ (2022)
- Li Z, "Climate warming increases the proportions of specific antibiotic resistance genes in natural soil ecosystems", J. Hazard Mater, 430, pp.128442~ (2022)
- MacFadden DR, "Antibiotic resistance increases with local temperature", Nat. Climate Change, 8, pp.510~514 (2018)
- Das A, "Management of diabetic foot in an Indian clinical setup : An opinion survey", Cureus, 12, pp.e8636~ (2020)
- Nicol MJ, "Antibacterial effects of low-temperature plasma generated by atmospheric-pressure plasma jet are mediated by reactive oxygen species", Sci. Rep, 10, pp.3066~ (2020)
- O'Toole GA, "Microtiter dish biofilm formation assay", J. Vis. Exp, 30, pp.2437~ (2011)
- Sestili P, "The fast-halo assay for the detection of DNA damage", Methods Mol. Biol, 1644, pp.75~93 (2017)
- Roopak Murali RE, "Cold atmospheric plasma(CAP)in wound healing : harnessing a dual-edged sword", Redox Experimental Medicine, 2024(1) (2024)
- Bhutani S, "Hyperbaric oxygen and wound healing", Indian J. Plast. Surg, 45, pp.316~324 (2012)
- Lee J, "The bacteriology of diabetic foot ulcers and infections and incidence of Staphylococcus aureus small colony variants", J. Med. Microbiol, 72 (2023)[DOI]
- Mai-Prochnow A, "Pseudomonas aeruginosa biofilm response and resistance to cold atmospheric pressure plasma is linked to the redox-active molecule phenazine", PLoS One, 10, pp.e0130373~ (2015)
- Ermolaeva SA, "Bactericidal effects of nonthermal argon plasma in vitro, in biofilms and in the animal model of infected wounds", J. Med. Microbiol, 60(Pt 1), pp.75~83 (2011)
- Mai-Prochnow A, "Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma", Sci. Rep, 6, pp.38610~ (2016)
- Kakoullis L, "Mechanisms of antibiotic resistance in important Gram-positive and Gram-negative pathogens and novel antibiotic solutions", Antibiotics(Basel), 10, pp.415~ (2021)
- Butaye P, "Mobile genes coding for efflux-mediated antimicrobial resistance in Gram-positive and Gram-negative bacteria", Int. J. Antimicrob. Agents, 22, pp.205~210 (2003)
- Baz A, "Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy", Biofilm, 5, pp.100123~ (2023)
- Dahle S, "Cold plasma for sustainable control of hygienically relevant biofilms. The interaction of plasma distance and exposure time", Int. J. Environ. Health Res, 34, pp.340~354 (2024)
- Akira S, "Toll-like receptors : critical proteins linking innate and acquired immunity", Nat. Immunol, 2, pp.675~680 (2001)
- Pandey S, "Microbial sensing by Toll-like receptors and intracellular nucleic acid sensors", Cold Spring Harb. Perspect. Biol, 7, pp.a016246~ (2014)
- Boehm D, "Safety implications of plasma-induced effects in living cells – a review of in vitro and in vivo findings", Biol. Chem, 400, pp.3~17 (2019)
- Jackson SP, "The DNA-damage response in human biology and disease", Nature, 461, pp.1071~1078 (2009)
- Stratmann B, "Effect of cold atmospheric plasma therapy vs standard therapy placebo on wound healing in patients with diabetic foot ulcers : A randomized clinical trial", JAMA Netw Open, 3, pp.e2010411~ (2020)
- Mirpour S, "Cold atmospheric plasma as an effective method to treat diabetic foot ulcers : A randomized clinical trial", Sci. Rep, 10, pp.10440~ (2020)
- Abu Rached N, "Cold plasma therapy in chronic wounds-A multicenter, randomized controlled clinical trial(Plasma on chronic wounds for epidermal regeneration study) : Preliminary results", J. Clin Med, 12, pp.5121~ (2023)