Impact of UV-C Irradiation on Bacterial Disinfection in a Drinking Water Purification System
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The supply of microbiological risk-free water is essential to keep food safety and public hygiene. And removal, inactivation, and destruction of microorganisms in drinking water are key for ensuring safety in the food industry. Ultraviolet-C (UV-C) irradiation is an attractive method for efficient disinfection of water without generating toxicity and adversely affecting human health. In this study, the disinfection efficiencies of UV-C irradiation on Shigella flexneri (Gram negative) and Listeria monocytogenes (Gram positive) at various concentrations in drinking water were evaluated using a water purifier. Their morphological and physiological characteristics after UV-C irradiation were observed using fluorescence microscopy and flow cytometry combined with live/dead staining. UV-C irradiation (254 nm wavelength, irradiation dose: 40 mJ/cm2 ) at a water flow velocity of 3.4 L/min showed disinfection ability on both bacteria up to 108 CFU/4 L. And flow cytometric analysis showed different physiological shift between S. flexneri and L. monocytogenes after UV-C irradiation, but no significant shift of morphology in both bacteria. In addition, each bacterium revealed different characteristics with time-course observation after UV-C irradiation: L. monocytogenes dramatically changed its physiological feature and seemed to reach maximum damage at 4 h and then recovered, whereas S. flexneri seemed to gradually die over time. This study revealed that UV-C irradiation of water purifiers is effective in disinfecting microbial contaminants in drinking water and provides basic information on bacterial features/responses after UV-C irradiation.
Abstract
The supply of microbiological risk-free water is essential to keep food safety and public hygiene. And removal, inactivation, and destruction of microorganisms in drinking water are key for ensuring safety in the food industry. Ultraviolet-C (UV-C) irradiation is an attractive method for efficient disinfection of water without generating toxicity and adversely affecting human health. In this study, the disinfection efficiencies of UV-C irradiation on Shigella flexneri (Gram negative) and Listeria monocytogenes (Gram positive) at various concentrations in drinking water were evaluated using a water purifier. Their morphological and physiological characteristics after UV-C irradiation were observed using fluorescence microscopy and flow cytometry combined with live/dead staining. UV-C irradiation (254 nm wavelength, irradiation dose: 40 mJ/cm2 ) at a water flow velocity of 3.4 L/min showed disinfection ability on both bacteria up to 108 CFU/4 L. And flow cytometric analysis showed different physiological shift between S. flexneri and L. monocytogenes after UV-C irradiation, but no significant shift of morphology in both bacteria. In addition, each bacterium revealed different characteristics with time-course observation after UV-C irradiation: L. monocytogenes dramatically changed its physiological feature and seemed to reach maximum damage at 4 h and then recovered, whereas S. flexneri seemed to gradually die over time. This study revealed that UV-C irradiation of water purifiers is effective in disinfecting microbial contaminants in drinking water and provides basic information on bacterial features/responses after UV-C irradiation.
참고문헌 (40)
- Kenefick RW, "Present Knowledge in Nutrition", pp.493~505 (2012), International Life Sciences Institute
- Cotruvo JA, "Drinking water standards and risk assessment", Regul. Toxicol. Pharmacol, 8, pp.288~299 (1988)
- Johnson KM, "Ultraviolet radiation and its germicidal effect in drinking water purification", J. Phytol, 2, pp.12~19 (2010)
- Fischer Walker CLF, "Diarrhea incidence in low- and middle-income countries in 1990 and 2010: a systematic review", BMC Public Health, 12, pp.220~ (2012)
- Palit A, "In-house contamination of potable water in urban slum of Kolkata, India: a possible transmission route of diarrhea", Water Sci. Technol, 66, pp.299~303 (2012)
- Process Water Subgroup of the European Hygienic Engineering & Design Group, "Safe and hygienic water treatment in food factories", Trends Food Sci. Technol, 18, pp.S93~S100 (2007)
- World Health Organization, "Guidelines for drinking-water quality, 4th ed", World Health Organization, ~ (2011)
- Jeon MJ, "Efficacy of UV-A, UV-B, and UV-C Irradiation on inactivation of foodborne pathogens in different neutralizing buffer solutions", LWT-Food Sci. Technol, 98, pp.591~597 (2018)
- Li GQ, "Comparison of UV-LED and low pressure UV for water disinfection:Photoreactivation and dark repair of Escherichia coli", Water Res, 126, pp.134~143 (2017)
- Song K, "Application of ultraviolet light-emitting diodes (UV-LEDs) for water disinfection: a review", Water Res, 94, pp.341~349 (2016)
- Bukhari Z, "Medium-pressure UV for oocyst inactivation", J. Am. Water Works Assoc, 91, pp.86~94 (1999)
- Choi Y, "The effects of UV disinfection on drinking water quality in distribution systems", Water Res, 44, pp.115~122 (2010)
- Clancy JL, "Using UV to inactivate Cryptosporidium", J. Am. Water Works Assoc, 92, pp.97~104 (2000)
- EPA, "Ultraviolet disinfection guidance manual", Office of Water, 35~59 (2003)
- Kalisvaart BF, "Re-use of wastewater: preventing the recovery of pathogens by using medium-pressure UV lamp technology", Water. Sci. Technol, 50, pp.337~344 (2004)
- Goto N, "Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics", Sci. Rep, 5, pp.11808~ (2015)
- Yokoyama H, "Structural biology of DNA (6–4) photoproducts formed by ultraviolet radiation and interactions with their binding proteins", Int. J. Mol. Sci, 15, pp.20321~20338 (2014)
- López MA, "Novel Food Processing Technologies", pp.405~421 (2005), CRC Press
- Franz CMAP, "UV-C-inactivation of microorganisms in naturally cloudy apple juice using novel inactivation equipment based on Dean vortex technology", Food Control, 20, pp.1103~1107 (2009)
- Yun J, "Fate of E. coli O157:H7, Salmonella spp. and potential surrogate bacteria on apricot fruit, following exposure to UV-C light", Int. J. Food Microbiol, 166, pp.356~363 (2013)
- Sobsey MD, "Point of use household drinking water filtration: a practical, effective solution for providing sustained access to safe drinking water in the developing world", Environ. Sci. Technol, 42, pp.4261~4267 (2008)
- Maciel PMF, "Household water purification system comprising cartridge filtration, UVC disinfection and chlorination to treat turbid raw water", J. Water Process Eng, 43, pp.102203~ (2021)
- Acheson DWK, "Encyclopedia of Microbiology", pp.365~381 (2009), Elsevier Inc
- Kirk MD, "World health organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal, and viral diseases, 2010: A Data Synthesis", PLoS Med, 12, pp.e1001921~ (2015)
- 인예원, "식품으로부터 쉬겔라 검출을 위한 분리배지 비교", 한국식품영양과학회지, 40(7), pp.1025~1031 (2011), 한국식품영양과학회
- Islam MA, "Handbook of Culture Media for Food and Water Microbiology", pp.287~297 (2012), Royal Society of Chemistry
- Bernbom N, "Listeria monocytogenes survival of UV-C radiation is enhanced by presence of sodium chloride, organic food material and by bacterial biofilm formation", Int. J. Food Microbiol, 147, pp.69~73 (2011)
- Gabriel AA, "Inactivation of Salmonella, E. coli and Listeria monocytogenes in phosphate-buffered saline and apple juice by ultraviolet and heat treatments", Food Control, 20, pp.443~446 (2009)
- Unluturk S, "Modeling inactivation kinetics of liquid egg white exposed to UV-C irradiation", Int. J. Food Microbiol, 142, pp.341~347 (2010)
- Nikogosyan DN, "Photolysis of aromatic amino acids in aqueous solution by nanosecond 248 and 193-nm laser light", J. Photochem. Photobiol. B Biol, 13, pp.219~234 (1992)
- Hallmich C, "Effect of pre- and post-UV disinfection conditions on photoreactivation of fecal coliforms in wastewater effluents", Water Res, 44, pp.2885~2893 (2010)
- Knudson GB, "Photoreactivation of UV-irradiated Legionella pneumophila and other Legionella species", Appl. Environ. Microbiol, 49, pp.975~980 (1985)
- Sinha RP, "UV-induced DNA damage and repair: a review", Photochem. Photobiol. Sci, 1, pp.225~236 (2002)
- Chang JCH, "UV inactivation of pathogenic and indicator microorganisms", Appl. Environ. Microbiol, 49, pp.1361~1365 (1985)
- Cheigh CI, "Comparison of intense pulsed light- and ultraviolet (UVC)-induced cell damage in Listeria monocytogenes and Escherichia coli O157:H7", Food Control, 25, pp.654~659 (2012)
- Gayán E, "Environmental and biological factors influencing the UV-C resistance of Listeria monocytogenes", Food Microbiol, 46, pp.246~253 (2015)
- Lu G, "UV inactivation of milk-related microorganisms with a novel electrodeless lamp apparatus", Eur. Food Res. Technol, 233, pp.79~87 (2011)
- Ghorbal SKB, "Changes in membrane fatty acid composition of Pseudomonas aeruginosa in response to UV-C radiations", Curr. Microbiol, 67, pp.112~117 (2013)
- Santos AL, "Effects of UV radiation on the lipids and proteins of bacteria studied by mid-infrared spectroscopy", Environ. Sci. Technol, 47, pp.6306~6315 (2013)
- Chourabi K, "UV-C adaptation of Shigella morphological, outer membrane proteins, secreted proteins, and lipopolysaccharides effects", Curr. Microbiol, 74, pp.1261~1269 (2017)
