본문 바로가기
KERD
논문KCI 등재

Increased Amino Acid Absorption Mediated by Lacticaseibacillus rhamnosus IDCC 3201 in High-Protein Diet-Fed Mice

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류생물학

초록

The use of dietary protein products has increased with interests in health promotion, and demand for sports supplements. Among various protein sources, milk protein is one of the most widely employed, given its economic and nutritional advantages. However, recent studies have revealed that milk protein undergoes fecal excretion without complete hydrolysis in the intestines. To increase protein digestibility, heating and drying were implemented; however, these methods reduce protein quality by causing denaturation, aggregation, and chemical modification of amino acids. In the present study, we observed that Lacticaseibacillus rhamnosus IDCC 3201 actively secretes proteases that hydrolyze milk proteins. Furthermore, we showed that co-administration of milk proteins and L. rhamnosus IDCC 3201 increased the digestibility and plasma concentrations of amino acids in a high-protein diet mouse model. Thus, food supplementation of L. rhamnosus IDCC 3201 can be an alternative strategy to increase the digestibility of proteins.

Abstract

The use of dietary protein products has increased with interests in health promotion, and demand for sports supplements. Among various protein sources, milk protein is one of the most widely employed, given its economic and nutritional advantages. However, recent studies have revealed that milk protein undergoes fecal excretion without complete hydrolysis in the intestines. To increase protein digestibility, heating and drying were implemented; however, these methods reduce protein quality by causing denaturation, aggregation, and chemical modification of amino acids. In the present study, we observed that Lacticaseibacillus rhamnosus IDCC 3201 actively secretes proteases that hydrolyze milk proteins. Furthermore, we showed that co-administration of milk proteins and L. rhamnosus IDCC 3201 increased the digestibility and plasma concentrations of amino acids in a high-protein diet mouse model. Thus, food supplementation of L. rhamnosus IDCC 3201 can be an alternative strategy to increase the digestibility of proteins.

참고문헌 (37)

  1. Kårlund A, "Protein supplements and their relation with nutrition, microbiota composition and health: is more protein always better for sportspeople?", Nutrients, 11, pp.829~ (2019)
  2. Ozer BH, "Functional milks and dairy beverages", Int. J. Dairy Technol, 63, pp.1~15 (2010)
  3. Zehetmeier M, "Does increasing milk yield per cow reduce greenhouse gas emissions? A system approach", Animal, 6, pp.154~166 (2012)
  4. Schaafsma G, "The protein digestibility-corrected amino acid score", J. Nutr, 130, pp.1865S~1867S (2000)
  5. Rebouillat S, "Potential applications of milk fractions and valorization of dairy by-products: A review of the state-of-the-art available data, outlining the innovation potential from a bigger data standpoint", J. Biomater. Nanobiotechnol, 6, pp.176~ (2015)
  6. "Whey protein powder market 2022: Detailed analysis of industry, potential growth, attractive valuation and growth forecast up to 2029"[URL]
  7. Goodman BE, "Insights into digestion and absorption of major nutrients in humans", Adv. Physiol. Educ, 34, pp.44~53 (2010)
  8. de la Fuente M, "Enzyme therapy: current challenges and future perspectives", Int. J. Mol. Sci, 22, pp.9181~ (2021)
  9. Schutz Y, "Protein turnover, ureagenesis and gluconeogenesis", Int. J. Vitam. Nutr. Res, 81, pp.101~107 (2011)
  10. Halper J, "Basic components of connective tissues and extracellular matrix: elastin, fibrillin, fibulins, fibrinogen, fibronectin, laminin, tenascins and thrombospondins", Adv. Exp. Med. Biol, 802, pp.31~47 (2014)
  11. Dupont D, "Milk proteins: Digestion and absorption in the gastrointestinal tract", pp.701~714 (2020), Elsevier
  12. van Lieshout GAA, "How processing may affect milk protein digestion and overall physiological outcomes: A systematic review", Crit. Rev. Food Sci. Nutr, 60, pp.2422~2445 (2020)
  13. Jäger R, "Probiotic administration increases amino acid absorption from plant protein: a placebo-controlled, randomized, double-blind, multicenter, crossover study", Probiotics Antimicrob. Proteins, 12, pp.1330~1339 (2020)
  14. Stecker RA, "Bacillus coagulans GBI-30, 6086 improves amino acid absorption from milk protein", Nutr. Metab, 17, pp.93~ (2020)
  15. Wang J, "Influence of probiotics on dietary protein digestion and utilization in the gastrointestinal tract", Curr. Protein Pept. Sci, 20, pp.125~131 (2019)
  16. Kim MS, "Enhanced ceramide production by Lactobacillus rhamnosus IDCC 3201 and its proposed mechanism", Appl. Biol. Chem, 64, pp.50~ (2021)
  17. Seemann T, "Prokka: rapid prokaryotic genome annotation", Bioinformatics, 30, pp.2068~2069 (2014)
  18. Jones P, "InterProScan 5: genome-scale protein function classification", Bioinformatics, 30, pp.1236~1240 (2014)
  19. Camacho C, "BLAST+: architecture and applications", BMC Bioinform, 10, pp.421~ (2009)
  20. Huerta-Cepas J, "eggNOG 4.5: a hierarchical orthology framework with improved functional annotations for eukaryotic, prokaryotic and viral sequences", Nucleic Acids Res, 44, pp.D286~D293 (2016)
  21. Sun SW, "Efficiency improvements on ninhydrin method for amino acid quantification", J. Food Compos. Anal, 19, pp.112~117 (2006)
  22. Déry O, "Proteinase-activated receptors: novel mechanisms of signaling by serine proteases", Am. J. Physiol. Cell Physiol, 274, pp.C1429~C1452 (1998)
  23. Khan MJ, "Why inclusion matters for Alzheimer’s disease biomarker discovery in plasma", J. Alzheimers Dis, 79, pp.1327~1344 (2021)
  24. Christensson C, "Cloning and expression of an oligopeptidase, PepO, with novel specificity from Lactobacillus rhamnosus HN001 (DR20)", Appl. Environ. Microbiol, 68, pp.254~262 (2002)
  25. Harwood CR, "The ins and outs of Bacillus proteases: activities, functions and commercial significance", FEMS Microbiol. Rev, 46, pp.fuab046~ (2022)
  26. Yokoyama T, "The Escherichia coli S2P intramembrane protease RseP regulates ferric citrate uptake by cleaving the sigma factor regulator FecR", J. Biol. Chem, 296, pp.100673~ (2021)
  27. Savijoki K, "Proteolytic systems of lactic acid bacteria", Appl. Microbiol. Biotechnol, 71, pp.394~406 (2006)
  28. Mayo B, "Effect of X-prolyl dipeptidyl aminopeptidase deficiency on Lactococcus lactis", Appl. Environ. Microbiol, 59, pp.2049~2055 (1993)
  29. Royle PJ, "Whey protein isolate and glycomacropeptide decrease weight gain and alter body composition in male Wistar rats", Br. J. Nutr, 100, pp.88~93 (2008)
  30. Kaewtapee C, "Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low-and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs", J. Anim. Sci. Biotechnol, 8, pp.37~ (2017)
  31. Wu G, "Functional amino acids in growth, reproduction, and health", Adv. Nutr, 1, pp.31~37 (2010)
  32. Li P, "Amino acids and immune function", Br. J. Nutr, 98, pp.237~252 (2007)
  33. Holeček M, "Serine metabolism in health and disease and as a conditionally essential amino acid", Nutrients, 14, pp.1987~ (2022)
  34. Alves A, "Glycine metabolism and its alterations in obesity and metabolic diseases", Nutrients, 11, pp.1356~ (2019)
  35. Stapleton PP, "Host defense‐a role for the amino acid taurine?", J. Parenter. Enteral. Nutr, 22, pp.42~48 (1998)
  36. Vettore LA, "Proline metabolism and redox; maintaining a balance in health and disease", Amino Acids, 53, pp.1779~1788 (2021)
  37. Konopelski P, "Biological effects of indole-3-propionic acid, a gut microbiota-derived metabolite, and its precursor tryptophan in mammals’ health and disease", Int. J. Mol. Sci, 23, pp.1222~ (2022)

관련 논문AI추천

Postbiotic Potential of Heat-Killed Lacticaseibacillus paracasei HP7 in Functional DyspepsiaJeong Ji-Woong, Kim Joo-Yun, Lee Daehyeop, Lee Jaehwan, Shim Jae-Jung한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Lactobacillus rhamnosus CBT-LR5 Improves Lipid Metabolism by Enhancing Vitamin AbsorptionChung Myung Jun, Kim Dong-Jin, Kim Tai Yeub, Ryu Yongku, Yoon Yeo-Sang한국미생물·생명공학회 · 한국미생물·생명공학회지2022 · 제50권 제4호Metabolic Regulation of Longevity and Immune Response in Caenorhabditis elegans by Ingestion of Lacticaseibacillus rhamnosus IDCC 3201 Using Multi-Omics Analysis김영훈, 김준모, 송민호, Choi Hyejin, Eor Ju Young, Kang An Na, Kim Hyung Wook, Kim Jong Nam, Kwak Min-Jin, Lee Daniel Junpyo, Lee Junbeom, Mun Daye, Oh Sangnam, Yang Jungwoo한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제5호Gut Microbiota Modulation by Lacticaseibacillus paracasei EFEL6501 Ameliorates Muscle AtrophyHan Nam Soo, Moon Jin Seok, Pyeon Geum Na, Seong Hyunbin한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Fermented Milk Containing Lacticaseibacillus rhamnosus SNU50430 Modulates Immune Responses and Gut Microbiota in Antibiotic-Treated MiceChoi Il-Dong, Jung Seong Eun, Kim Woon-Ki, Ko GwangPyo, Lee Cheonghoon, Park SungJun, Yoon Sunghyun한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제6호Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat DietBoxing Yin, Dawei Chen, Feixiang Guo, Haiqing Zhao, Jiadi Huang, Ruixia Gu, Xia Chen, Yujun Huang, Yun Wu, Zhenquan Yang한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2015 · 제25권 제5호

이 간행물의 다른 논문 (14건)