Fermented Milk Containing Lacticaseibacillus rhamnosus SNU50430 Modulates Immune Responses and Gut Microbiota in Antibiotic-Treated Mice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Antibiotics are used to control infectious diseases. However, adverse effects of antibiotics, such as devastation of the gut microbiota and enhancement of the inflammatory response, have been reported. Health benefits of fermented milk are established and can be enhanced by the addition of probiotic strains. In this study, we evaluated effects of fermented milk containing Lacticaseibacillus rhamnosus (L. rhamnosus) SNUG50430 in a mouse model with antibiotic treatment. Fermented milk containing 2 × 105 colony-forming units of L. rhamnosus SNUG50430 was administered to six weekold female BALB/c mice for 1 week. Interleukin (IL)-10 levels in colon samples were significantly increased (P < 0.05) compared to water-treated mice, whereas interferon-gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) were decreased, of mice treated with fermented milk containing L. rhamnosus SNUG50430-antibiotics-treated (FM+LR+Abx-treated) mice. Phylum Firmicutes composition in the gut was restored and the relative abundances of several bacteria, including the genera Coprococcus and Lactobacillus, were increased in FM+LR+Abx-treated mice compared to PBS+Abxtreated mice. Interestingly, abundances of genus Coprococcus and Lactobacillus were positively correlated with IL-5 and IL-10 levels (P < 0.05) in colon samples and negative correlated with IFN-γ and TNF-α levels in serum samples (P < 0.001). Acetate and butyrate were increased in mice with fermented milk and fecal microbiota of FM+LR+Abx-treated mice were highly enriched with butyrate metabolism pathway compared to water-treated mice (P < 0.05). Thus, fermented milk containing L. rhamnosus SNUG50430 was shown to ameliorate adverse health effects caused by antibiotics through modulating immune responses and the gut microbiota.
Abstract
Antibiotics are used to control infectious diseases. However, adverse effects of antibiotics, such as devastation of the gut microbiota and enhancement of the inflammatory response, have been reported. Health benefits of fermented milk are established and can be enhanced by the addition of probiotic strains. In this study, we evaluated effects of fermented milk containing Lacticaseibacillus rhamnosus (L. rhamnosus) SNUG50430 in a mouse model with antibiotic treatment. Fermented milk containing 2 × 105 colony-forming units of L. rhamnosus SNUG50430 was administered to six weekold female BALB/c mice for 1 week. Interleukin (IL)-10 levels in colon samples were significantly increased (P < 0.05) compared to water-treated mice, whereas interferon-gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) were decreased, of mice treated with fermented milk containing L. rhamnosus SNUG50430-antibiotics-treated (FM+LR+Abx-treated) mice. Phylum Firmicutes composition in the gut was restored and the relative abundances of several bacteria, including the genera Coprococcus and Lactobacillus, were increased in FM+LR+Abx-treated mice compared to PBS+Abxtreated mice. Interestingly, abundances of genus Coprococcus and Lactobacillus were positively correlated with IL-5 and IL-10 levels (P < 0.05) in colon samples and negative correlated with IFN-γ and TNF-α levels in serum samples (P < 0.001). Acetate and butyrate were increased in mice with fermented milk and fecal microbiota of FM+LR+Abx-treated mice were highly enriched with butyrate metabolism pathway compared to water-treated mice (P < 0.05). Thus, fermented milk containing L. rhamnosus SNUG50430 was shown to ameliorate adverse health effects caused by antibiotics through modulating immune responses and the gut microbiota.
참고문헌 (48)
- Mårild K, "Antibiotic exposure and the development of coeliac disease : a nationwide case–control study", BMC Gastroenterol, 13, pp.109~ (2013)
- Chen IL, "The effects of antibiotic exposure on asthma in children with atopic dermatitis", Sci. Rep, 11, pp.8526~ (2021)
- Fenneman AC, "Antibiotics in the pathogenesis of diabetes and inflammatory diseases of the gastrointestinal tract", Nat. Rev. Gastroenterol. Hepatol, 20, pp.81~100 (2023)
- Knoop KA, "Antibiotics promote inflammation through the translocation of native commensal colonic bacteria", Gut, 65, pp.1100~1109 (2015)
- Looft T, "Collateral effects of antibiotics on mammalian gut microbiome", Gut Microbes, 3, pp.463~467 (2012)
- Gupta R, "Role of alternatives to antibiotics in mitigating the antimicrobial resistance crisis", Indian J. Med. Res, 156, pp.464~477 (2020)
- Mohsen S, "Update on the adverse effects of antimicrobial therapies in community practice", Can. Fam. Physician, 66, pp.651~659 (2020)
- Hadjimbei E, "Beneficial effects of yoghurts and probiotic fermented milks and their functional food potential", Foods, 11, pp.2691~ (2022)
- Sionek B, "Traditional and new microorganisms in lactic acid fermentation of food", Fermentation, 9, pp.1019~ (2023)
- Wang S, "Fermented milk supplemented with probiotics and prebiotics can effectively alter the intestinal microbiota and immunity of host animals", J. Dairy Sci, 95, pp.4813~4822 (2012)
- Jiang T, "Bifidobacterium longum 070103 fermented milk improve glucose and lipid metabolism disorders by regulating gut microbiota in mice", Nutrients, 14, pp.4050~ (2022)
- Santiago-López L, "The effects of consuming probiotic-fermented milk on the immune system : A review of scientific evidence", Int. J. Dairy Technol, 68, pp.153~165 (2015)
- Matsuoka K, "Efficacy of Bifidobacterium breve fermented milk in maintaining remission of ulcerative colitis", Dig. Dis. Sci, 63, pp.1910~1919 (2018)
- Feng C, "Oral administration of pasteurized probiotic fermented milk alleviates dextran sulfate sodium-induced inflammatory bowel disease in rats", J. Funct. Foods, 94, pp.105140~ (2022)
- Liu J, "Functions of gut microbiota metabolites, current status and future perspectives", Aging Dis, 13, pp.1106~ (2022)
- Wong JMW, "Colonic health : Fermentation and short chain fatty acids", J. Clin. Gastroenterol, 40, pp.235~243 (2006)
- Portincasa P, "Gut microbiota and short chain fatty acids : Implications in glucose homeostasis", Int. J. Mol. Sci, 23, pp.1105~ (2022)
- Parada Venegas D, "Short chain fatty acids(SCFAs)-mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases", Front. Immunol, 10, pp.277~ (2019)
- Yip W, "Butyrate shapes immune cell fate and function in allergic asthma", Front. Immunol, 12, pp.628453~ (2021)
- Nagpal R, "Human-origin probiotic cocktail increases short-chain fatty acid production via modulation of mice and human gut microbiome", Sci. Rep, 8, pp.12649~ (2018)
- Ochoa-Repáraz J, "Role of gut commensal microflora in the development of experimental autoimmune encephalomyelitis", J. Immunol, 183, pp.6041~6050 (2009)
- Kim W-K, "Administration of Lactobacillus paracasei strains improves immunomodulation and changes the composition of gut microbiota leading to improvement of colitis in mice", J. Funct. Foods, 52, pp.565~575 (2019)
- Walters W, "Improved bacterial 16S rRNA gene(V4 and V4-5)and fungal internal transcribed spacer marker gene primers for microbial community surveys", mSystems, 1, pp.e00009-00015~ (2016)
- Caporaso JG, "QIIME allows analysis of highthroughput community sequencing data", Nat. Methods, 7, pp.335~336 (2010)
- Yoon S, "Distinct changes in Microbiota-mediated intestinal metabolites and immune responses induced by different antibiotics", Antibiotics, 11, pp.1762~ (2022)
- Langille MG, "Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences", Nat. Biotechnol, 31, pp.814~821 (2013)
- Han DH, "Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice", Sci. Rep, 12, pp.9640~ (2022)
- David LA, "Diet rapidly and reproducibly alters the human gut microbiome", Nature, 505, pp.559~563 (2014)
- Ward NC, "IL-2", CD25 : a long-acting fusion protein that promotes immune tolerance by selectively targeting the IL-2 receptor on regulatory T cells, 2018, pp.201, 2579~2592
- Reyes-Díaz A, "Milk fermented by specific Lactobacillus strains regulates the serum levels of IL-6, TNF-α and IL-10 cytokines in a LPS-stimulated murine model", Nutrients, 10, pp.691~ (2018)
- Yan S, "Bifidobacterium longum subsp. longum YS108R fermented milk alleviates DSS induced colitis via anti-inflammation, mucosal barrier maintenance and gut microbiota modulation", J. Funct. Foods, 73, pp.104153~ (2020)
- Wang Y, "Lactobacillus rhamnosus GG reduces hepatic TNFα production and inflammation in chronic alcohol-induced liver injury", J. Nutr. Biochem, 24, pp.1609~1615 (2013)
- Khailova L, "Lactobacillus rhamnosus GG and Bifidobacterium longum attenuate lung injury and inflammatory response in experimental sepsis", PLoS One, 9, pp.e97861~ (2014)
- Zhang J, "Lactobacillus rhamnosus GG induced protective effect on allergic airway inflammation is associated with gut microbiota", Cell. Immunol, 332, pp.77~84 (2018)
- Khan TJ, "Association of gut dysbiosis with intestinal metabolites in response to antibiotic treatment", Hum. Microbiome J, 11, pp.100054~ (2019)
- Ramirez J, "Antibiotics as major disruptors of gut microbiota", Front. Cell. Infect. Microbiol, 10, pp.572912~ (2020)
- Galdeano CM, "Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice", BMC Gastroenterol, 11, pp.1~14 (2011)
- Okoniewski A, "The Role of fermented dairy products on gut microbiota composition", Fermentation, 9, pp.231~ (2023)
- Oh NS, "Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces", PLoS One, 13, pp.e0192021~ (2018)
- Han SK, "Lactobacillus rhamnosus HDB1258 modulates gut microbiotamediated immune response in mice with or without lipopolysaccharide-induced systemic inflammation", BMC Microbiol, 21, pp.146~ (2021)
- Rizzatti G, "Proteobacteria : a common factor in human diseases", Biomed Res. Int, 2017, pp.9351507~ (2017)
- Jiang X, "Dissemination of antibiotic resistance genes from antibiotic producers to pathogens", Nat. Commun, 8, pp.15784~ (2017)
- Lisko DJ, "Effects of dietary yogurt on the healthy human gastrointestinal(GI)microbiome", Microorganisms, 5, pp.6~ (2017)
- Le Roy CI, "Yoghurt consumption is associated with changes in the composition of the human gut microbiome and metabolome", BMC Microbiol, 22, pp.39~ (2022)
- Wu H-J, "The role of gut microbiota in immune homeostasis and autoimmunity", Gut Microbe, 3, pp.4~14 (2012)
- Zheng D, "Interaction between microbiota and immunity in health and disease", Cell Res, 30, pp.492~506 (2020)
- Vacca M, "The controversial role of human gut lachnospiraceae", Microorganisms, 8, pp.573~ (2020)
- Vinolo MAR, "Regulation of inflammation by short chain fatty acids", Nutrients, 3, pp.858~876 (2011)