Characterization of Natural Compounds as Inhibitors of NS1 Endonuclease from Canine Parvovirus Type 2
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Canine parvovirus type 2 (CPV-2) has high morbidity and mortality rates in canines. Nonstructural protein 1 (NS1) of CPV-2 has endonuclease activity, initiates viral DNA replication, and is highly conserved. Thus, it is a promising target for antiviral inhibitor development. We overexpressed a 41.9 kDa active recombinant endonuclease in Escherichia coli and designed a nicking assay using carboxyfluorescein and quencher-linked ssDNA as substrates. The optimal temperature and pH of the endonuclease were 37°C and pH 7, respectively. Curcumin, bisdemethoxycurcumin, demethoxycurcumin, linoleic acid, tannic acid, and α-tocopherol inhibited CPV-2 NS1 endonuclease with IC50 values of 0.29 to 8.03 μM. The extracted turmeric, yerba mate, and sesame cake suppressed CPV-2 NS1 endonuclease with IC50 values of 1.48, 7.09, and 52.67 μg/ml, respectively. The binding affinity between curcumin, the strongest inhibitor, and CPV-2 NS1 endonuclease by molecular docking was −6.4 kcal/mol. Curcumin inhibited CPV-2 NS1 endonuclease via numerous hydrophobic interactions and two hydrogen bonds with Lys97 and Pro111 in the allosteric site. These results suggest that adding curcuminoids, linoleic acid, tannic acid, α-tocopherol, extracted turmeric, sesame cake, and yerba to the diet could prevent CPV-2 infection.
Abstract
Canine parvovirus type 2 (CPV-2) has high morbidity and mortality rates in canines. Nonstructural protein 1 (NS1) of CPV-2 has endonuclease activity, initiates viral DNA replication, and is highly conserved. Thus, it is a promising target for antiviral inhibitor development. We overexpressed a 41.9 kDa active recombinant endonuclease in Escherichia coli and designed a nicking assay using carboxyfluorescein and quencher-linked ssDNA as substrates. The optimal temperature and pH of the endonuclease were 37°C and pH 7, respectively. Curcumin, bisdemethoxycurcumin, demethoxycurcumin, linoleic acid, tannic acid, and α-tocopherol inhibited CPV-2 NS1 endonuclease with IC50 values of 0.29 to 8.03 μM. The extracted turmeric, yerba mate, and sesame cake suppressed CPV-2 NS1 endonuclease with IC50 values of 1.48, 7.09, and 52.67 μg/ml, respectively. The binding affinity between curcumin, the strongest inhibitor, and CPV-2 NS1 endonuclease by molecular docking was −6.4 kcal/mol. Curcumin inhibited CPV-2 NS1 endonuclease via numerous hydrophobic interactions and two hydrogen bonds with Lys97 and Pro111 in the allosteric site. These results suggest that adding curcuminoids, linoleic acid, tannic acid, α-tocopherol, extracted turmeric, sesame cake, and yerba to the diet could prevent CPV-2 infection.
참고문헌 (49)
- Nandi S, "Canine parvovirus: current perspective", Indian J. Virol, 21, pp.31~44 (2010)
- Parrish CR, "Host range relationships and the evolution of canine parvovirus", Vet. Microbiol, 69, pp.29~40 (1999)
- Hoelzer K, "The emergence of parvoviruses of carnivores", Vet. Res, 41, pp.39~ (2010)
- Sykes JE, "Canine parvovirus infections and other viral enteritides", Canine Feline Infect. Dis, 2014, pp.141~151 (2014)
- Decaro N, "Canine parvovirus vaccination and immunisation failures: are we far from disease eradication?", Vet. Microbiol, 247, pp.108760~ (2020)
- Parrish CR, "Antigenic relationships between canine parvovirus type-2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal-antibodies", Arch. Virol, 72, pp.267~278 (1982)
- Decaro N, "Canine parvovirus-a review of epidemiological and diagnostic aspects, with emphasis on type 2c", Vet. Microbiol, 155, pp.1~12 (2012)
- Reed AP, "Nucleotide sequence and genome organization of canine parvovirus", J. Virol, 62, pp.266~276 (1988)
- Chen B, "Molecular epidemiological survey of canine parvovirus circulating in china from 2014 to 2019", Pathogens, 10, pp.588~ (2021)
- Zhao W, "Administration with vaccinia virus encoding canine parvovirus 2 vp2 elicits systemic immune responses in mice and dogs", Viral Immunol, 33, pp.434~443 (2020)
- Chang D, "Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system", BMC Vet. Res, 16, pp.202~ (2020)
- Hao X, "The changes in canine parvovirus variants over the years", Int. J. Mol. Sci, 23, pp.11540~ (2022)
- Remond M, "Partial DNA cloning and sequencing of a canine parvovirus vaccine strain: application of nucleic acid hybridization to the diagnosis of canine parvovirus disease", Arch. Virol, 127, pp.257~269 (1992)
- Tewary SK, "Structure of the NS1 protein N-terminal origin recognition", nickase domain from the emerging human bocavirus, 2013, pp.87, 11487~11493
- Parrish CR, "The origins of new pandemic viruses: the acquisition of new host ranges by canine parvovirus and influenza A viruses", Annu. Rev. Microbiol, 59, pp.553~586 (2005)
- Arora R, "Canine parvovirus and its non-structural gene 1 as oncolytic agents: mechanism of action and induction of anti-tumor immune response", Front. Oncol, 11, pp.648873~ (2021)
- Xu P, "Endonuclease activity inhibition of the NS1 protein of parvovirus B19 as a novel target for antiviral drug development", Antimicrob. Agents Chemother, 63, pp.e01879-01818~ (2018)
- Viana LM, "Natural food for domestic animals: a national and international technological review", Res. Vet. Sci, 130, pp.11~18 (2020)
- Gee NR, "Dogs supporting human health and well-being: a biopsychosocial approach", Front. Vet. Sci, 8, pp.630465~ (2021)
- Webb B, "Comparative protein structure modeling using MODELLER", Curr. Protoc. Bioinformatics, 54, pp.5~6 (2016)
- Tian W, "CASTp 3.0: computed atlas of surface topography of proteins", Nucleic Acids Res, 46, pp.W363~W367 (2018)
- Trott O, "AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading", J. Comput. Chem, 31, pp.455~461 (2010)
- Eberhardt J, "AutoDock Vina 1.2. 0: New docking methods, expanded force field, and python bindings", J. Chem. Inf. Model, 61, pp.3891~3898 (2021)
- Nguyen TTH, "Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris", Biotechnol. Lett, 34, pp.831~838 (2012)
- Wallace AC, "LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions", Protein Eng, 8, pp.127~134 (1995)
- Tattersall P, "Rolling hairpin model for replication of parvovirus and linear chromosomal DNA", Nature, 263, pp.106~109 (1976)
- Luo Y, "Human parvovirus B19: a mechanistic overview of infection and DNA replication", Future Virol, 10, pp.155~167 (2015)
- Wang X, "Development of a monoclonal antibody against canine parvovirus NS1protein and investigation of NS1 dynamics and localization in CPV-infected cells", Protein Expr. Purif, 174, pp.105682~ (2020)
- Kumar GR, "In vitro expression studies of non structural 1 protein of Canine Parvo virus 2 by polyclonal antiserum raised against CPV2-NS1 protein expressed in Escherichia coli as an antigen", Indian J. Exp. Biol, 50, pp.618~624 (2012)
- Sandur SK, "Curcumin, demethoxycurcumin, bisdemethoxycurcumin, tetrahydrocurcumin and turmerones differentially regulate anti-inflammatory and anti-proliferative responses through a ROSindependent mechanism", Carcinogenesis, 28, pp.1765~1773 (2007)
- Galli GM, "Feed addition of curcumin to laying hens showed anticoccidial effect, and improved egg quality and animal health", Res. Vet. Sci, 118, pp.101~106 (2018)
- Jaguezeski AM, "Addition of curcumin to the diet of dairy sheep improves health, performance and milk quality", Anim. Feed Sci. Technol, 246, pp.144~157 (2018)
- Molosse V, "Diet supplemented with curcumin for nursing lambs improves animal growth, energetic metabolism, and performance of the antioxidant and immune systems", Small Ruminant Res, 170, pp.74~81 (2019)
- Campigotto G, "Dog food production using curcumin as antioxidant: effects of intake on animal growth, health and feed conservation", Arch. Anim. Nutr, 74, pp.397~413 (2020)
- Ak T, "Antioxidant and radical scavenging properties of curcumin", Chem. Biol. Interact, 174, pp.27~37 (2008)
- Adamczak A, "Curcumin, a natural antimicrobial agent with strain-specific activity", Pharmaceuticals (Basel), 13, pp.153~ (2020)
- Aggarwal S, "Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of IκBα kinase and Akt activation", Mol. Pharmacol, 69, pp.195~206 (2006)
- Wacal C, "Fatty acid composition of sesame (Sesamum indicum L.)seeds in relation to yield and soil chemical properties on continuously monocropped upland fields converted from paddy fields", Agronomy, 9, pp.801~ (2019)
- Melo D, "Whole or defatted sesame seeds (Sesamum indicum L.)? The effect of cold pressing on oil and cake quality", Foods, 10, pp.2108~ (2021)
- Obeidat BS, "Replacing soybean meal with sesame meal in the diets of lactating awassi ewes suckling single lambs: nutrient digestibility, milk production, and lamb growth", Animals-Basel, 9, pp.157~ (2019)
- Additives EPo, "Scientific Opinion on the safety and efficacy of tannic acid when used as feed flavouring for all animal species", EFSA J, 12, pp.3828~ (2014)
- Yang K, "Gallnut tannic acid exerts anti-stress effects on stress-induced inflammatory response, dysbiotic gut microbiota, and alterations of serum metabolic profile in beagle dogs", Front. Nutr, 9, pp.847966~ (2022)
- Gülçin İ, "Radical scavenging and antioxidant activity of tannic acid", Arab. J. Chem, 3, pp.43~53 (2010)
- Pizarro F, "Factors which modify the nutritional state of iron: tannin content of herbal teas", Arch. Latinoam. Nutr, 44, pp.277~280 (1994)
- Berté KA, "Chemical composition and antioxidant activity of yerbamate (Ilex paraguariensis A. St.-Hil., Aquifoliaceae) extract as obtained by spray drying", J. Agric. Food Chem, 59, pp.5523~5527 (2011)
- Butiuk AP, "Study of the chlorogenic acid content in yerba mate (Ilex paraguariensis St. Hil.):Effect of plant fraction, processing step and harvesting season", J. Appl. Res. Med. Aromat. Plants, 3, pp.27~33 (2016)
- Mateos R, "LC–MSn characterization of saponins in mate (Ilex paraguariens, St. Hil) and their quantification by HPLC-DAD", J. Food Compos. Anal, 63, pp.164~170 (2017)
- Nguyen TTH, "Facile preparation of water soluble curcuminoids extracted from turmeric (Curcuma longa L.) powder by using steviol glucosides", Food Chem, 214, pp.366~373 (2017)
- Marchiori MS, "Curcumin in the diet of quail in cold stress improves performance and egg quality", Anim. Feed Sci. Technol, 254, pp.114192~ (2019)
