Direct Monitoring of Membrane Fatty Acid Changes and Effects on the Isoleucine/ Valine Pathways in an ndgR Deletion Mutant of Streptomyces coelicolor
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
NdgR, a global regulator in soil-dwelling and antibiotic-producing Streptomyces, is known to regulate branched-chain amino acid metabolism by binding to the upstream region of synthetic genes. However, its numerous and complex roles are not yet fully understood. To more fully reveal the function of NdgR, phospholipid fatty acid (PLFA) analysis with gas chromatography-mass spectrometry (GC-MS) was used to assess the effects of an ndgR deletion mutant of Streptomyces coelicolor. The deletion of ndgR was found to decrease the levels of isoleucine- and leucine-related fatty acids but increase those of valine-related fatty acids. Furthermore, the defects in leucine and isoleucine metabolism caused by the deletion impaired the growth of Streptomyces at low temperatures. Supplementation of leucine and isoleucine, however, could complement this defect under cold shock condition. NdgR was thus shown to be involved in the control of branched-chain amino acids and consequently affected the membrane fatty acid composition in Streptomyces. While isoleucine and valine could be synthesized by the same enzymes (IlvB/N, IlvC, IlvD, and IlvE), ndgR deletion did not affect them in the same way. This suggests that NdgR is involved in the upper isoleucine and valine pathways, or that its control over them differs in some respect.
Abstract
NdgR, a global regulator in soil-dwelling and antibiotic-producing Streptomyces, is known to regulate branched-chain amino acid metabolism by binding to the upstream region of synthetic genes. However, its numerous and complex roles are not yet fully understood. To more fully reveal the function of NdgR, phospholipid fatty acid (PLFA) analysis with gas chromatography-mass spectrometry (GC-MS) was used to assess the effects of an ndgR deletion mutant of Streptomyces coelicolor. The deletion of ndgR was found to decrease the levels of isoleucine- and leucine-related fatty acids but increase those of valine-related fatty acids. Furthermore, the defects in leucine and isoleucine metabolism caused by the deletion impaired the growth of Streptomyces at low temperatures. Supplementation of leucine and isoleucine, however, could complement this defect under cold shock condition. NdgR was thus shown to be involved in the control of branched-chain amino acids and consequently affected the membrane fatty acid composition in Streptomyces. While isoleucine and valine could be synthesized by the same enzymes (IlvB/N, IlvC, IlvD, and IlvE), ndgR deletion did not affect them in the same way. This suggests that NdgR is involved in the upper isoleucine and valine pathways, or that its control over them differs in some respect.
참고문헌 (39)
- Bentley SD, "Complete genome sequence of the model actinomycete Streptomyces coelicolor A3 (2)", Nature, 417, pp.141~147 (2002)
- Rudd BA, "Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3 (2)", Microbiology, 114, pp.35~43 (1979)
- Bhatia SK, "Medium engineering for enhanced production of undecylprodigiosin antibiotic in Streptomyces coelicolor using oil palm biomass hydrolysate as a carbon source", Bioresour. Technol, 217, pp.141~149 (2016)
- Hesketh A, "Primary and secondary metabolism, and post‐translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor", Mol. Microbiol, 46, pp.917~932 (2002)
- Bibb MJ, "Regulation of secondary metabolism in streptomycetes", Curr. Opin. Microbiol, 8, pp.208~215 (2005)
- Liu G, "Molecular regulation of antibiotic biosynthesis in Streptomyces", Microbiol. Mol. Biol. Rev, 77, pp.112~143 (2013)
- Xu Z, "Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products", Eng. Microbiol, pp.100022~ (2022)
- Lee BR, "The role of NdgR in glycerol metabolism in Streptomyces coelicolor", Bioprocess Biosys. Eng, 40, pp.1573~1580 (2017)
- Yang YH, "NdgR, an IclR-like regulator involved in amino-acid-dependent growth, quorum sensing, and antibiotic production in Streptomyces coelicolor", Appl. Microbiol. Biotechnol, 82, pp.501~511 (2009)
- Martín JF, "Cascades and networks of regulatory genes that control antibiotic biosynthesis", Subcell. Biochem, 64, pp.115~138 (2012)
- Kim SH, "NdgR, a common transcriptional activator for methionine and leucine biosynthesis in Streptomyces coelicolor", J. Bacteriol, 194, pp.6837~6846 (2012)
- Huang H, "Molecular mechanisms underlying the function diversity of transcriptional factor IclR family", Cell. Signal, 24, pp.1270~1275 (2012)
- Angara RK, "An IclR like protein from mycobacteria regulates leuCD operon and induces dormancy-like growth arrest in Mycobacterium smegmatis", Tuberculosis, 108, pp.83~92 (2018)
- Kim JN, "Genome-scale analysis reveals a role for NdgR in the thiol oxidative stress response in Streptomyces coelicolor", BMC Genomics, 16, pp.1~11 (2015)
- Lee BR, "Increased vulnerability to physical stress by inactivation of NdgR in Streptomyces coelicolor", Appl. Biochem. Biotechnol, 175, pp.3673~3682 (2015)
- Klein W, "Cold shock response of Bacillus subtilis: isoleucine-dependent switch in the fatty acid branching pattern for membrane adaptation to low temperatures", J. Bacteriol, 181, pp.5341~5349 (1999)
- Kaplun A, "Isoleucine starvation caused by sulfometuron methyl in Salmonella typhimurium measured by translational frameshifting", Microbiology, 148, pp.713~717 (2002)
- Hou J, "Discovery of an unusual fatty acid amide from the ndgRyo gene mutant of marinederived Streptomyces youssoufiensis", Mar. Drugs, 17, pp.12~ (2019)
- Vestal JR, "Lipid analysis in microbial ecology", Bioscience, 39, pp.535~541 (1989)
- Albanesi D, "Lipid Signaling and Metabolism", pp.1~22 (2020), Elsevier
- Drenovsky RE, "Comparison of phospholipid fatty acid (PLFA) and total soil fatty acid methyl esters (TSFAME) for characterizing soil microbial communities", Soil Biol. Biochem, 36, pp.1793~1800 (2004)
- Fernandes MF, "Comparison of whole-cell fatty acid (MIDI) or phospholipid fatty acid (PLFA) extractants as biomarkers to profile soil microbial communities", Microb. Ecol, 66, pp.145~157 (2013)
- Vorbeck ml, "Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography", J. Lipid Res, 6, pp.3~6 (1965)
- Lee HS, "Tung oil-based production of high 3-hydroxyhexanoate-containing terpolymer poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) using engineered Ralstonia eutropha", Polymers, 13, pp.1084~ (2021)
- Kaneda T, "Iso-and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance", Microb. Rev, 55, pp.288~302 (1991)
- Sandoval-Calderón M, "Knowns and unknowns of membrane lipid synthesis in streptomycetes", Biochimie, 141, pp.21~29 (2017)
- Neinast M, "Branched chain amino acids", Ann. Rev. Physiol, 81, pp.139~ (2019)
- Zhang YM, "Membrane lipid homeostasis in bacteria", Nat. Rev. Microbiol, 6, pp.222~ (2008)
- Smittle R, "Relationship of cellular fatty acid composition to survival of Lactobacillus bulgaricus in liquid nitrogen", Appl. Environ. Microbiol, 27, pp.738~743 (1974)
- Beranová J, "Metabolic control of the membrane fluidity in Bacillus subtilis during cold adaptation", Biochim. Biophys. Acta, 1778, pp.445~453 (2008)
- Song HS, "Naringenin as an antibacterial reagent controlling of biofilm formation and fatty acid metabolism in MRSA", bioRxiv, ~ (2020)
- Bligh EG, "A rapid method of total lipid extraction and purification", Can. J. Biochem. Physiol, 37, pp.911~917 (1959)
- Choi TR, "Effects of a Δ-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties", J. Ind. Microbiol. Biotechnol, 47, pp.1045~1057 (2020)
- Bhatia SK, "Effect of synthetic and food waste-derived volatile fatty acids on lipid accumulation in Rhodococcus sp. YHY01 and the properties of produced biodiesel", Energy Convers. Manag, 192, pp.385~395 (2019)
- Cropp TA, "Fatty-acid biosynthesis in a branched-chain α-keto acid dehydrogenase mutant of Streptomyces avermitilis", Can. J. Microbiol, 46, pp.506~514 (2000)
- Mercier R, "Crucial role for membrane fluidity in proliferation of primitive cells", Cell Rep, 1, pp.417~423 (2012)
- Sprusansky O, "The bkdR gene of Streptomyces coelicolor is required for morphogenesis and antibiotic production and encodes a transcriptional regulator of a branched-chain amino acid dehydrogenase complex", J. Bacteriol, 187, pp.664~671 (2005)
- Denoya CD, "A second branched-chain alphaketo acid dehydrogenase gene cluster (bkdFGH) from Streptomyces avermitilis: its relationship to avermectin biosynthesis and the construction of a bkdF mutant suitable for the production of novel antiparasitic avermectins", J. Bacteriol, 177, pp.3504~3511 (1995)
- Hemilä H, "Secretory S complex of Bacillus subtilis: sequence analysis and identity to pyruvate dehydrogenase", J. Bacteriol, 172, pp.5052~5063 (1990)