Transcriptional Analysis of Genes Involved in Ectopic Sporulation in Streptomyces griseus
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Two Streptomyces griseus strains, a wild-type strain and an A-factor-dependent transcriptional activator mutant strain harboring multiple copies of a gene, dasA, that encodes a substrate-binding protein of the ATP-binding cassette transporter, showed severe ectopic sporulation of young substrate hyphae in response to glucose. The effect of dasA overexpression on the ectopic sporulation of Streptomyces strains was evaluated by comparing the transcriptomes of the strain harboring multiple copies of dasA and a strain harboring empty vector. By DNA microarray, 4 genes (SGR794, SGR2469, SGR3656, and SGR3657) and 3 clusters (SGR795-797, SGR2377-2378, and SGR6997-6998) were differentially expressed by more than 2-fold in S. griseus strains harboring dasA. The DNA microarray result was validated by low-resolution S1 nuclease mapping.
Abstract
Two Streptomyces griseus strains, a wild-type strain and an A-factor-dependent transcriptional activator mutant strain harboring multiple copies of a gene, dasA, that encodes a substrate-binding protein of the ATP-binding cassette transporter, showed severe ectopic sporulation of young substrate hyphae in response to glucose. The effect of dasA overexpression on the ectopic sporulation of Streptomyces strains was evaluated by comparing the transcriptomes of the strain harboring multiple copies of dasA and a strain harboring empty vector. By DNA microarray, 4 genes (SGR794, SGR2469, SGR3656, and SGR3657) and 3 clusters (SGR795-797, SGR2377-2378, and SGR6997-6998) were differentially expressed by more than 2-fold in S. griseus strains harboring dasA. The DNA microarray result was validated by low-resolution S1 nuclease mapping.
참고문헌 (17)
- Akanuma G, "Dynamic changes in the extracellular proteome caused by absence of a pleiotropic regulator AdpA in Streptomyces griseus", Mol. Microbiol, 73, pp.898~912 (2009)
- 지원재, "Functional Analysis of SGR4635-Induced Enhancement of Pigmented Antibiotic Production in Streptomyces lividans", The Journal of Microbiology, 49(5), pp.828~833 (2011), 한국미생물학회
- Hara H, "DNA microarray analysis of global gene regulation by A-factor in Streptomyces griseus", Microbiology, 155, pp.2197~2210 (2009)
- Hillerich B, "A new GntR family transcriptional regulator in Streptomyces coelicolor is required for morphogenesis and antibiotic production and controls transcription of an ABC transporter in response to carbon source", J. Bacteriol, 188, pp.7477~7487 (2006)
- Horinouchi S., "Mining and polishing of the treasure trove in the bacterial genus streptomyces", Biosci. Biotechnol. Biochem, 71, pp.283~299 (2007)
- Jiang H, "Characterization of ssfR and ssgA, two genes involved in sporulation of Streptomyces griseus", J. Bacteriol, 182, pp.5521~5529 (2000)
- Kato JY, "Transcriptional control by A-factor of two trypsin genes in Streptomyces griseus", J. Bacteriol, 187, pp.286~295 (2005)
- Keiser BJ, "The ram-dependence of Streptomyces lividans differentiation is bypassed by copper", J. Mol. Microbiol . Biotechnol, 2, pp.565~574 (2000)
- Li M, "Enhancement of avermectin and ivermectin production by overexpression of the maltose ATP-binding cassette transporter in Streptomyces avermitilis", Bioresour. Technol, 101, pp.9228~9235 (2010)
- Nazari B, "Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil", Appl. Environ. Microbiol, 79, pp.707~713 (2013)
- Ochi K, "An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor", Mol. Microbiol, 30, pp.107~119 (1998)
- Ohnishi Y, "Deprogrammed sporulation in Streptomyces", FEMS Microbiol. Lett, 216, pp.1~7 (2002)
- Ohnishi Y, "Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO13350", J. Bacteriol, 190, pp.4050~4060 (2008)
- Rigali S, "The sugar phosphotransferase system of Streptomyces coelicolor is regulated by the GntR-family regulator DasR and links N-acetylglucosamine metabolism to the control of development", Mol. Microbiol, 61, pp.1237~1251 (2006)
- Daniel Rozas, "A Novel Two-Component System Involved in the Transition to Secondary Metabolism in Streptomyces coelicolor", PLoS ONE, 7(2), pp.e31760~ (2012), Public Library of Science (PLoS)[DOI]
- Seo JW, "ATP-binding cas-sette transport system involved in regulation of morphological differentiation in response to glucose in Streptomyces griseus", J. Bacteriol, 184, pp.91~103 (2002)
- Yu Z, "A genome-wide transcriptomic analysis reveals diverse roles of the two-component system DraR-K in the physiological and morphological differentiation of Streptomyces coelicolor", Appl. Microbiol. Biotechnol, 98, pp.9351~9363 (2014)