Ham's F-10 배양액에 첨가된 태아제대혈청과 EDTA가 수정란의 분할에 미치는 영향

Abstract

It is the most important to select optimal culture conditions to promote safe embryo growth in the technique of human in vitro fertilization and embryo transfer. It has been shown that the addition of biologic fluids, such as blood serum, of various origins, improved fertilization and early cleavage rates in numerous species. The purpose of this study is to attempt to measure developmental potential of mouse eggs fertilized and cleaved in Ham's F10 culture medium containing a chelating agent, EDTA and fetal cord serum. In this study, we selected 40 female mice and 20 male mice, and investigated optimal serum concentration' for mouse embryo growth. Two cell stage mouse embryos were cultured in Ham's F-10 medium, Ham's F-10 medium with various concentrations of EDTA, or Ham's F-10 medium with EDTA and 10% human cord serum. Developmental ratios to morula in Ham's F-10 medium containing various concentrations of EDTA and/or 10% fetal cord serum were significantly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to blastocyst in Ham's F-10 containing 10% fetal cord serum and 5011M or 100µM EDTA were significantly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to morula in Ham's F-10 containing 10% fetal cord serum and 100µM EDTA were significantly higher than in Ham's F-10 with 10% fetal cord serum used commonly in many human IVF centers (p<0.05). Developmental ratio to blastocyst in Ham's F-10 containing 10% fetal cord serum and 100µM EDTA was significantly higher than in Ham's F-10 with 200µM EDTA (p<0.05). In summary, embryo development to morula and blastocyst was significantly higher in the presence of human cord serum or EDTA than in the unsupplemented medium. The most significantly development to morula and blastocyst was obtained at Ham’s F-10 medium with 100µM concentration of EDTA and 10% fetal cord serum. These results suggest that Ham’s F-10 medium containing 10% fetal cord serum and optimal concentrations of EDTA significantly promoted early cleavage of mouse zygotes, and these will be useful as basic data for the selection of culture medium in human in vitro fertilization.