알레르기성 비염 마우스에서 Stem Cell Factor의 비강내 투여 효과

Abstract

Background and Objectives：The pathogenesis of allergic rhinitis has not been fully understood. The role of stem cell factor (SCF)instillation of SCF in mice with experimentaly-induced allergic rhinitis. Materials and Method：Male BALB-c mice were intraperitonially sensitized to ovalbumin mixed with aluminum hydroxide and were challenged with aerosols of ovalbumin. Recombinant murine SCF was directly instilled to both nasal cavities. Sneezing and nose-rubbing act of the mice were checked by symptom scores. Nasal lavage fluid (NLF) was collected and histamine levels in the NLF were measured. The eosinophils in the inferior turbinate of the mice were counted at the coronal section of the skull. Results：The symptom scores checked 1 hour after intranasal instillation of 50 nM SCF and 50 nM SCF were 29± 8 (mean± S.D.) and 31± 10, respectively. They were significantly diferent from those of the control group (18± 5). Histamine levels of NLF colected 1 hour after intranasal instillation of 500 nM SCF were 3.62 1.8 nM and were significantly diferent from those of the control group (1.31± 0.4 nM)of eosinophils in the inferior turbinate of mice counted 24 hours after intranasal instillation of 500 nM SCF was 17.6± 3.2 and was significantly diferent from those of the control group (11.2± 3.5). Conclusion：Stem cell factor may play an important role in the pathogenesis of allergic rhinitis. (Korean J Otolaryngol 2003 ;46 :110-4)