두경부암세포주에서 Cisplatin 및 5-FU 처리 후 Telomerase 발현에 대한 연구
The Change of Telomerase Activity by Cisplatin and 5-FU in Head and Neck Cancer Cell Lines
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Abstract
Background and Objectives：Telomerase activity appears to be associated with cell imortalisation and malignant progres-sion. Telomerase activity has been found in the majority of human tumours, but not in most somatic cells or tisues. We studied the change in the telomerase activity by cisplatin and 5-fluorouracil (5-FU) in head and neck cancer cel lines Subjects and Methods： (PNUH-12, SNU-899, HEp-2) were studied. Cell cytotoxi-city and change of telomerase activity by 5-FU and cisplatin treatment were assessed by MT and TRAP assays. Results：The telomerase activity was detected in the head and neck cancer cell lines. After the cisplatin treatment, the telomerase activity was decreased in a concentration-dependent manner. However, after 5-FU treatment, the telomerase activity showed a slight decrease or no change at the concentration similar to that of cisplatin in the inhibition of tumor growth. Conclusion：Our results can
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