호흡기 상피세포에서 Vasoactive Intestinal Peptide에 의한 MUC2/5AC 생성
Vasoactive Intestinal Peptide Induces MUC2/5AC Synthesis in Human Airway Epithelial Cells
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Abstract
Background and Objectives：Vasointestinal peptide (VIP) is an important neurotransmitter involved in the regulation of mucus n MUC2/5AC genes expresion and mucin secretion in human airway epithelial cells. Materials and Method：The mRNA levels of MUC2/5AC genes and mucin secretion were determined by RT-PCR and the imunoblot method in cultured human airway NCI-H292 epithelial cells. Results：VIP (10-6- 10-10 M) induced MUC2/5AC gene expression and mucin secretion in a reverse MUC2/5AC was 10-10 M of VIP. Acti-nomycin D inhibited the VIP-mediated MUC2/5AC gene expression and mucin secretion, but cycloheximide did not. Budesonide attenuated the VIP-mediated MUC2/5AC genes expression and mucin secretion. RU-486, a glucocorticoid receptor antagonist, restored the inhibitory efect of budesonide. Conclusion：These results sugest that VIP regulates MUC2/5AC gene expression MUC2/5AC genes expression and mucin secretion through the glucocorticoid receptor.
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