본문 바로가기
KERD
논문KCI 등재

사람 정상 코점막 상피세포에서 겔 형성 점소(Gel-Forming Mucin)분비에 대한 Prostaglandin E₂의 효과

Effect of Prostaglandin E₂ on Gel-forming Mucin Secretion inNormal Human Nasal Epithelial Cells

김창훈|김현직|김규보|장현종|윤주헌|김진국
대한이비인후과학회 (Korean Society of Otorhinolaryngology-Head and Neck Surgery)|2006.03|대한이비인후과학회지 두경부외과학 (Korean Journal of Otorhinolaryngology-Head and Neck Surgery)|제49권 제3호|pp. 307-312

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

PGE2·점소·분비·PG 수용체.Prostaglandin E2·Mucin·Secretion·Prostaglandin receptor.
주제분류이비인후과학

초록

Abstract

Background and Objectives：Recently, Prostaglandin E2 (PGE2) was found to induce MUC5AC production via an agonist of E-prostanoid (EP2/EP4), but not EP1/EP3, in normal human airway epithelium. However, the receptor that mediates MUC5AC has not been determined. This study was aimed to investigate the MUC5AC mucin gene and mucin secretion by PGE2 and its receptors in cultured normal human nasal epithelial cells. Materials and Method：After treatment with PGE2 and/or PGE2 antagonist, gel-forming mucin mRNA expression was determined by reverse transcription-polymerase chain reaction. Total mucin and MUC5AC mucin levels were measured using an immuno-dot blotting assay. Results：PGE2 increased the MUC5AC gene expressions and MUC5AC mucin, but not the expressions of other gel-forming mucin genes. An EP2 receptor antagonist (AH6809) did not suppress the PGE2-induced MUC5AC gene expression or MUC5AC mucin. However, an EP4 receptor antagonist (AH23848) significantly suppressed the level of PGE2-induced MUC5AC gene expression and MUC5AC mucin. Conclusion：These findings indicate that PGE2 induces MUC5AC gene expression and mucin secretion via EP4 receptor in cultured normal human nasal epithelialcells. (Korean J Otolaryngol 2006;49:307-12)

참고문헌 (18)

  1. "Characterization of mucins from cultured normal human tracheobronchialepithelial cells", 278, pp.1118~1128 (2000)
  2. "Effect of dexamethasone on Muc5ac mucinproduction by promary airway goblet cells", 288, pp.52~60 (2005)
  3. "Prostanoid receptors: Structures,properties, and functions", 79, pp.1193~1226 (1999)
  4. "Interleukin-1β induced mucin production in human airway epitheliumis mediated by cyclooxygenase-2, prostaglandin E2 receptors, cyclicAMP-protein kinase A signaling", 66, pp.337~346 (2004)
  5. "Interleukin-1β and tumor necrosis factor-α induce MUC5AC overexpressionthrough a mechanism involving ERK", p38 mitogen-activatedprotein kinase-MSK1-CREB activation in human airway epithelial cells, 2003, pp.278, 23243~23250
  6. "Effects of TNF-αand IL-1β on mucin IL-6 and IL-8 in passage-2 normal humannasal epithelial cells", 119, pp.905~910 (1999)
  7. "Secretory differentiationof serially passaged normal human nasal epithelial cells by retinoicacid:Expression of mucin and lysozyme", 109, pp.594~601 (2000)
  8. "Regulation of thesecretory phenotype of human airway epithelium by retinoic acid, triiodothyronine,and extracellular matrix", 16, pp.724~731 (1997)
  9. "Secretory differention ofserially passaged normal human nasal epithelial cells by retinoic acid:Expression of mucin and lysozyme", 109, pp.594~601 (2000)
  10. "Prostaglandin E2 receptors of the EP2 and EP4subtypes regulate activation and differentiation of mouse B lymphocytesto IgE-secreting cells", 93, pp.10973~10983 (1996)
  11. "Interleukin-1induces MUC2 and MUC5AC synthesis through cyclooxygenase-2 in NCI-H292 cells", 62, pp.1112~1118 (2002)
  12. "Prostaglandin E2 induces MUC8 gene expression via a mechanisminvolving ERK MAPK", RSK1, pp.2005, 280[DOI]
  13. "Changes in expression of 15-lipoxygenaseand prostaglandin-H synthase during differentiation of humantracheobrochial epithelial cells", 18, pp.662~669 (1998)
  14. "Regulation of MUC5AC mucin secretion and airway surface liquidmetabolism by IL-1β in human bronchial epithelia", 286, pp.320~330 (2004)
  15. "Regulation of human airwaymucins by acrolein and inflammatory mediators", 276, pp.549~555 (1999)
  16. "EP4 receptor mediation of prostaglandinE2-stimulated mucus secretion by rabbit gastric epithelialcells", 58, pp.1997~2002 (1999)
  17. "Prostaglandin E2-stimulates rat and human colonicmucin exocytosis via the EP4 receptor", 117, pp.1352~1362 (1999)
  18. "EP2 and EP4 prostanoid receptor signaling", 74, pp.143~153 (2003)

관련 논문AI추천

사람 코점막과 배양된 코점막 상피세포에서 15-Lipoxygenase와 Cyclooxygenase-2의 발현김경수, 김선구, 김지훈, 김창훈, 윤주헌, 장정현대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2003 · 제46권 제8호사람 정상 코점막 상피세포에서 배양기간에 따른 분비 및 섬모세포로의 분화김민호, 김현준, 남지인, 윤주헌, 이정권, 이정준대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2003 · 제46권 제3호호흡기상피세포에서 Leukotriene 수용체를 통한 MUC2/5AC 점액유전자의 발현과 점액분비김용대, 배창훈, 서영중, 송시연, 예상백, 이재억대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2004 · 제47권 제11호사람 코점막과 배양된 코점막 상피세포에서 Nonsteroidal Anti-Inflammatory Drug-Activated Gene-1(NAG-1)의 발현김경수, 김선구, 김정홍, 김창훈, 신지현, 윤주헌대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2003 · 제46권 제5호식염수 세척이 비강 점액분비에 미치는 영향： 생체 내 및 생체 외 실험김부일, 김정수, 송선희, 이규엽, 장희상, 정상식대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2003 · 제46권 제11호호흡기 상피세포에서 Vasoactive Intestinal Peptide에 의한 MUC2/5AC 생성곽동석, 김용대, 송계원, 송시연, 신재흔, 이형중, 장근영대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2004 · 제47권 제7호

이 간행물의 다른 논문 (7건)

후각신경상피세포의 분화와 재생김정훈pp. 248-256사람 중이점막상피세포에서 Uridine-5’ Triphosphate에 의한칼슘 이동과 점액분비에 미치는 카페인의 효과최재영, 박헌이, 손은진, 김성헌, 정상호, 윤주헌, 신중욱pp. 263-265견치와 천공과 회전식 흡입기를 이용한상악동 내시경수술의 합병증 분석김효진, 김기현, 이용만, 고윤우, 김시찬, 이재용, 변장열pp. 303-306이하선 절제술시 Harmonic Scalpel사용의 유용성고윤우, 이종대, 신재민, 조성훈, 이승원, 김기현, 박재홍pp. 313-318당뇨환자에서 심경부감염에 대한 임상적 고찰임상철, 이준규pp. 323-327
원문 보기