비용조직에서 Heme Oxygenase-1 발현에 대한 면역조직학적 연구
A Immunohistochemical Study of Expression of Inducible Isoform of Heme Oxygenase on Human Nasal Polyps
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Abstract
Background and Objectives：Nasal polyp is non-neoplastic, chronic inflammatory disease of the nasal mucosa. Oxidative stress may be related to the pathogenesis of nasal polyp. Oxidative stress may be potential inducers of heme oxygenase (HO-1) expression. To illustrate the role of the HO-1 in the nasal polyp, we investigated the expressive pattern of the HO-1 in the nasal polyp and the influence of corticosteroids on its expression. Subjects and Method：The study materials were 28 specimens of nasal polyp (steroid user：13 cases, steroid non-user：15 cases) taken from 28 patients during endoscopic sinus surgery and 11 cases of normal nasal mucosa of the inferior turbinate as control. The HO-1 expression was assessed by immunohistochemical staining. Results：The HO-1 was mainly expressed on non-epithelial cells in the lamina propria of nasal polyps in 20/28 (71.4%). The expression of HO-1 was positive in 11 out of 13 (84.6%) and 9 out of 15 (60%) subjects for steroid user and steroid non-user group, respectively. The inflammatory cells were almost identified as macrophage by using a macrophage specific marker (CD68+). The HO-1 was not expressed in normal control at all. There was no significant difference in the expression of HO-1 between the steroid user group and the non-user group. Conclusion：HO-1, as an important endogenous antioxidant enzyme, may play a protective role in the pathogenesis of nasal polyp. Steroid may not regulate the HO-1 expression in the nasal polyp.
참고문헌 (18)
- "Epithelial cell proliferation in nasal polyps could be upregulated by platelet-derived growth factor", 106, pp.578~83 (1996)
- "Nasal polyp-derived superoxide anion:Dose-dependent inhibition by nitric oxide and pathophysiological implications", 163, pp.145~51 (2001)
- "Role of Free radicals and antioxidants in nasal polyps", 114, pp.1200~3 (2004)
- "Free radical damage in nasal polyp tissue", 124, pp.570~2 (2001)
- "Heme oxygenase and the kidney", 21, pp.307~21 (2002)
- "Expression of heme oxygenase isoenzymes 1 and 2 in normal and asthmatic airways effect of inhaled corticosteroids", 162, pp.1912~8 (2000)
- "Downregulation of the human heme oxygenase gene by glucocorticoids and identification of 56b regulatory elements", 218, pp.759~65 (1996)
- "Regulation of heme oxygenase-2 by glucocorticoids in neonatal rat brain:Characterization of a functional glucocorticoid response element", 1351, pp.89~104 (1997)
- "Heme oxygenase inhibits human airway smooth muscle proliferation via a bilirubin-dependent modulation of ERK1", 2 phosphorylation, 2003, pp.278, 27160~8
- "Heme oxygenase isoform expression in cellular and antibody-mediated models of acute inflammation in the rat", 190, pp.627~34 (2000)
- "and implication of a novel stress-inducible protein in oxidant-induced lung injury", pp.9~1 19
- "A review of recent research", pp.201~14 (1999), Dietary carotenoids and certain cancers
- "Overexpression of heme oxygenase-1 in human pulmonary epithelial cells results in cell growth arrest and increased resistance to hyperoxia", 93, pp.10393~8 (1996)
- "Hyperoxic regulation of lung heme oxygenase in neonatal rats", 40, pp.815~21 (1996)
- "Expression and modulatory effects of heme oxygenase in acute inflammation in the rat", pp.2~20 (1995)
- "Heme oxygenase-1 is an antifibrogenic protein in human hepatic myofibroblasts", 125, pp.460~9 (2003)
- "Raised levels of exhaled carbon monooxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma:A new marker of oxidative stress", 53, pp.668~72 (1998)
- "Negative regulation of human heme oxygenase in microvessel endothelial cells by dexamethasone Heme oxygenase isoform regulation by dexamethasone"