비용종 상피세포와 섬유아세포 동시배양 모델에서 시토카인의 발현

Abstract

Background and Objectives：fluid with sparse fibrous cells, few mucous glands and proliferation of stromal and epithelial cells. There have been many studies done separately about epithelial cels, fibroblasts, and other inflammatory cells in nasal polyps. However, there is no usable study model for the cell-to-cell interaction. Therefore, we tried to make a new model for the study of nasal polyp by coculture of epithelial cells and fibroblasts. Subjects and Method：Nasal polyp was colected during endoscopic sinus surgery. Almost half of them were used for epithelial cell culture and the remaining portion was used for fibroblast culture in the same polyp. Using a transwel insert, fibroblasts were placed in the botom and epithelial cells were placed on the insert, respectively. RNA were isolated both from epithelial cels and fibroblasts of 12, 24, and 48 hours coculture. The expressions of IL-8, IL-6, and eotaxin from cocul-tured cels were compared with cels that were cultured alone by semiquantitative RT-PCR. Results：IL-8 was highly expressed in fibroblasts than in epithelial cells, especially in cocultured cells. The expression of IL-6 in fibroblasts was much higher epithelial cells, especially in cocultured fibroblasts. Eotaxin is weakly expressed in fibroblasts but not in epithelial cells. Conclusion：These findings indicate that cocultured cells show diferent expression of the mRNA of various cytokines compared with cul-tured cels alone. And this coculture system may be more ideal than the mono-cel culture system for the study of cel-to-cel in-teractions and to reveal the pathogenesis of nasal polyposis. (Korean J Otolaryngol 2007 ;50 :134-8)