기니아 피그 고막에서 성체신경줄기세포의 분리 배양
Isolation and Culture of Adult Neural Stem Cells from Guinea Pig Tympanie Membrane
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초록
Tympanic membrane perforation is an important clinical problem found in various populations of the world. In large number of cases, acute traumatic perforations heal spontaneously, and in the healing process, stem cells appear to play an important role. However, no studies have been reported regarding somatic stem cells in the tympanic membrane. Herein, we tried to show that guinea pig’s tympanic membrane contains cells that display the characteristic features of stem cells. Materials and Method：The tympanic membrane was obtained from the guinea pig. The cells were cultured in a medium with epidermal growth factor (EGF) and fibroblast growth factor (FGF). Proliferating cells were checked with stem cell markers, bromodeoxyuridine (BrdU) and nestin. Differentiated cells from stem cells are checked with βIII tubulin and S-100. Results：We observed that some of the cultured cells from the tympanic membrane were stained with both stem cell markers, BrdU and nestin. And we observed that these cells differentiated into neuron and gilal cells, which expressed βIII tubulin and S-100, respectively. Conclusion： These results suggest that the tympanic membrane of guinea pigs may have neural stem cells. Further study is needed for finding the origin of stem cells. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:28-32)
Abstract
Tympanic membrane perforation is an important clinical problem found in various populations of the world. In large number of cases, acute traumatic perforations heal spontaneously, and in the healing process, stem cells appear to play an important role. However, no studies have been reported regarding somatic stem cells in the tympanic membrane. Herein, we tried to show that guinea pig’s tympanic membrane contains cells that display the characteristic features of stem cells. Materials and Method：The tympanic membrane was obtained from the guinea pig. The cells were cultured in a medium with epidermal growth factor (EGF) and fibroblast growth factor (FGF). Proliferating cells were checked with stem cell markers, bromodeoxyuridine (BrdU) and nestin. Differentiated cells from stem cells are checked with βIII tubulin and S-100. Results：We observed that some of the cultured cells from the tympanic membrane were stained with both stem cell markers, BrdU and nestin. And we observed that these cells differentiated into neuron and gilal cells, which expressed βIII tubulin and S-100, respectively. Conclusion： These results suggest that the tympanic membrane of guinea pigs may have neural stem cells. Further study is needed for finding the origin of stem cells. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:28-32)
참고문헌 (11)
- Li H, "Pluripotent stem cells from the adult mouse inner ear", Nat Med, 9(10), pp.1293~1299 (2003)
- von Unge M, "Embryonic stem cells enhance the healing of tympanic membrane perforations", Int J Ped Otorhinolaryngol, 67(3), pp.215~219 (2003)
- Wang WQ, "Epidermal stem cells in the tympanic membrane", Zhonghua Er Bi Yan Hou Ke Za Zhi, 39(12), pp.712~716 (2004)
- Ozkaptan Y, "Effect of fibroblast growth factor on the healing process of tympanic membrane perforations in an animal model", Eur Arch Otorhinolaryngol, 254(1), pp.2~5 (1997)
- Mondain M, "Histological study of the healing of traumatic tympanic membrane perforation after basic fibroblast growth factor application", Laryngoscope, 103(3), pp.312~318 (1993)
- Rahman A, "Healing time, long term result and effects of stem cell treatment in acute tympanic membrane perforation", Int J Ped Otorhinolaryngol, 71(7), pp.1129~1137 (2007)
- Forge A, "Ultrastructural evidence for hair cell regeneration in the mammalian inner ear", Science, 259(5101), pp.1616~1619 (1993)
- Jiang Y, "Ortiz- Gonzalez XR, et al. Pluripotency of mesenchymal stem cells derived from adult marrow", Nature, 418(6893), pp.41~49 (2002)
- Kopen GC, "Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains", Proc Natl Acad Sci U S A, 96(19), pp.10711~10716 (1999)
- Woodbury D, "Adult rat and human bone marrow stromal cells differentiate into neurons", J Neurosci Res, 61(4), pp.364~370 (2000)
- Watters K, "Feasibility of treating hearing disorders with stem cells: Update", Ear Nose Throat J, 83(10), pp.688~689 (2004)