인공기관 재료로서 다공성 폴리우레탄 스캐폴드의 유용성
Porous Polyurethane Scaffold as a Tracheal Prosthesis Material
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초록
Background and ObjectivesZZThe purpose of this study was to confirm the usefulness of the porous polyurethane scaffold as a tracheal prosthesis for repair of partial tracheal defect. Materials and MethodZZA porous polyurethane (PU) scaffold was manufactured by salt-leaching method. Polyethylene glycol (PEG) was grafted onto the surface of the PU scaffold following surface ozonation treatment. Scanning electro-microscopy (SEM) was then performed to evaluate the morphklogy. For the biocompatibility evaluation, a 5×5 mm sized PU scaffold was implanted into the rabbit’s ear. A histological examination was performed with the tissue one month later. Polymerase chain reaction for tumor necrosis factor (TNF)-α, interleukin (IL)-6, procollagen, fibromodulin, fibronectin, and actin was also performed. The tensile strength was measured and compared with beagle’s trachea to evaluate the mechanical property of the prosthesis. ResultsZZThe effect of ozonation and surface grafting with PEG was on the porous scaffold was confirmed by SEM and X-ray photoelectron spectroscopy. The histological examination showed a large amount of fibrous tissue and capillary had grown through the pores of the porous scaffold. The inflammatory reaction was not so severe. TNF-α and IL-6 were slightly elevated in the PU scaffold transplanted tissue. However, the concentration levels of the other cytokines were similar between transplanted tissue and normal tissue. The tensile strength was very similar with the beagle’s normal trachea. ConclusionZZThe polyethylene grafted polyurethane scaffold is a good candidate prosthesis for tracheal reconstruction.
Abstract
Background and ObjectivesZZThe purpose of this study was to confirm the usefulness of the porous polyurethane scaffold as a tracheal prosthesis for repair of partial tracheal defect. Materials and MethodZZA porous polyurethane (PU) scaffold was manufactured by salt-leaching method. Polyethylene glycol (PEG) was grafted onto the surface of the PU scaffold following surface ozonation treatment. Scanning electro-microscopy (SEM) was then performed to evaluate the morphklogy. For the biocompatibility evaluation, a 5×5 mm sized PU scaffold was implanted into the rabbit’s ear. A histological examination was performed with the tissue one month later. Polymerase chain reaction for tumor necrosis factor (TNF)-α, interleukin (IL)-6, procollagen, fibromodulin, fibronectin, and actin was also performed. The tensile strength was measured and compared with beagle’s trachea to evaluate the mechanical property of the prosthesis. ResultsZZThe effect of ozonation and surface grafting with PEG was on the porous scaffold was confirmed by SEM and X-ray photoelectron spectroscopy. The histological examination showed a large amount of fibrous tissue and capillary had grown through the pores of the porous scaffold. The inflammatory reaction was not so severe. TNF-α and IL-6 were slightly elevated in the PU scaffold transplanted tissue. However, the concentration levels of the other cytokines were similar between transplanted tissue and normal tissue. The tensile strength was very similar with the beagle’s normal trachea. ConclusionZZThe polyethylene grafted polyurethane scaffold is a good candidate prosthesis for tracheal reconstruction.
참고문헌 (21)
- Grillo HC, "Postintubation tracheal stenosis treatment and results", J Thorac Cardiovasc Surg, 109(3), pp.486~493 (1995)
- Grillo HC, "Tracheal replacement: a critical review", Ann Thorac Surg, 73(6), pp.1995~2004 (2002)
- Neville WE, "Clinical experience with the silicone tracheal prosthesis", J Thorac Cadiovasc Surg, 99(4), pp.604~613 (1990)
- Cull DL, "Tracheal reconstruction with polytetrafluoroethylene graft in dogs", Ann Thorac Surg, 50(6), pp.899~901 (1990)
- 김진훈, "Replacement of a tracheal defect with a tissue-engineered prosthesis: Early results from animal experiments", Journal of Thoracic and Cardiovascular Surgery, 128(1), pp.124~129, Mosby Inc.
- 김한수, "Development of an Artificial Tracheal Prosthesis: a Semicircular Shape Polyurethane Scaffold", 조직공학과 재생의학, 8(5), pp.439~445 (2011), 한국조직공학과 재생의학회
- Thibeault SL, "In Vivo engineering of the vocal fold ECM with injectable HA hydrogels-late effects on tissue repair and biomechanics in a rabbit model", J Voice, 25(2), pp.249~253 (2011)
- Chia SH, "Tissue-engineered human nasal septal cartilage using the alginate-recovered-chondrocyte method", Laryngoscope, 114(1), pp.38~45 (2004)
- Klein AM, "Polyglycolic acid", poly-L-lactic acid copolymer use in laryngotracheal reconstruction: a rabbit model, 2005, pp.115(4), 583~587
- Fuchs JR, "Fetal tracheal augmentation with cartilage engineered from bone marrow-derived mesenchymal progenitor cells", J Pediatr Surg, 38(6), pp.984~987 (2003)
- "Wikipedia free encyclopedia Web site" (2006)[URL]
- Jacobs JR, "Investigations into tracheal prosthetic reconstruction", Laryngoscope, 98(11), pp.1239~1245 (1988)
- Bjellerup M, "Novel method for training skin flap surgery: polyurethane foam dressing used as a skin equivalent", Dermatol Surg, 31(9 Pt 1), pp.1107~1111 (2005)
- Du YJ, "In vivo rabbit acute model tests of polyurethane catheters coated with a novel antithrombin-heparin covalent complex", Thromb Haemost, 94(2), pp.366~372 (2005)
- Verma S, "Nitric oxide-eluting polyurethanes--vascular grafts of the future?", N Engl J Med, 353(7), pp.730~731 (2005)
- Jefic D, "A single center experience in the use of polyurethaneurea arteriovenous grafts", Nephrol News Issues, 19(8), pp.44~47 (2005)
- Ramrattan NN, "Assessment of tissue ingrowth rates in polyurethane scaffolds for tissue engineering", Tissue Eng, 11(7-8), pp.1212~1223 (2005)
- Scherer MA, "Experimental bioprosthetic reconstruction of the trachea", Arch Otorhinolaryngol, 243(4), pp.215~223 (1986)
- Desai NP, "Biological responses to polyethylene oxide modified polyethylene terephthalate surfaces", J Biomed Mater Res, 25(7), pp.829~843 (1991)
- Lee JH, "Protein-resistant surfaces prepared by PEO-containing block copolymer surfactants", J Biomed Mater Res, 23(3), pp.351~368 (1989)
- Oosterom R, "Adhesion performance of UHMWPE after different surface modification techniques", Med Eng Phys, 28(4), pp.323~330 (2006)