옴나리스Ⓡ에 의한 비용상피세포 염증반응 및 점액유전자 발현 억제
The Effect of OmnarisⓇ on the Inflammatory Reaction and Mucin Gene Expression from Nasal Polyp Epithelial Cells
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Background and Objectives Glucocorticoids are a potent anti-inflammatory agent. The au-thors conducted this study to investigate the effect of Omnaris® on suppression of inflammation induction and mucin gene expression in nasal polyp epithelial cells. Subjects and Method Primary nasal polyp epithelial cells were stimulated by 5 ug/mL of streptococcal enterotoxin B (SEB) and lipopolysaccharide (LPS). To determine the effects of Omnaris®, cells were pretreated with 200, 100, 10, 1 ng/mL of Omnaris®. The anti-inflammatory effect of epithelial cells were confirmed by measuring interleukin (IL)-6, IL-8, and granulocyte-macrophage colony stimulating factor (GM-CSF), and mucin gene expressions were determined by real time PCR for MUC4, MUC5AC, MUC5B, MUC8. Results SEB and LPS enhanced the production of IL-6, IL-8, and GM-CSF from nasal polyp epithelial cells. The increased cytokine levels were significantly suppressed by Omnaris® at 100 and 10 ng/mL. The expressions of MUC4, MUC5AC, MUC5B, MUC8 mRNA, and MUC4 mRNA were increased by SEB and LPS, respectively. The increased expression of these mucin genes were significantly suppressed by 100, 10, and 1 ng/mL of Omnaris®. Conclusion Omnaris® significantly suppressed the production of chemical mediators and mucin gene expression, which indicated that Omnaris® is effective in improving and treating inflammatory diseases in the nasal cavity. Korean J Otorhinolaryngol-Head Neck Surg 2014;57(9):603-9
Abstract
Background and Objectives Glucocorticoids are a potent anti-inflammatory agent. The au-thors conducted this study to investigate the effect of Omnaris® on suppression of inflammation induction and mucin gene expression in nasal polyp epithelial cells. Subjects and Method Primary nasal polyp epithelial cells were stimulated by 5 ug/mL of streptococcal enterotoxin B (SEB) and lipopolysaccharide (LPS). To determine the effects of Omnaris®, cells were pretreated with 200, 100, 10, 1 ng/mL of Omnaris®. The anti-inflammatory effect of epithelial cells were confirmed by measuring interleukin (IL)-6, IL-8, and granulocyte-macrophage colony stimulating factor (GM-CSF), and mucin gene expressions were determined by real time PCR for MUC4, MUC5AC, MUC5B, MUC8. Results SEB and LPS enhanced the production of IL-6, IL-8, and GM-CSF from nasal polyp epithelial cells. The increased cytokine levels were significantly suppressed by Omnaris® at 100 and 10 ng/mL. The expressions of MUC4, MUC5AC, MUC5B, MUC8 mRNA, and MUC4 mRNA were increased by SEB and LPS, respectively. The increased expression of these mucin genes were significantly suppressed by 100, 10, and 1 ng/mL of Omnaris®. Conclusion Omnaris® significantly suppressed the production of chemical mediators and mucin gene expression, which indicated that Omnaris® is effective in improving and treating inflammatory diseases in the nasal cavity. Korean J Otorhinolaryngol-Head Neck Surg 2014;57(9):603-9
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