Expression and Role of Calcitonin Gene-Related Peptide in Patients With Chronic Rhinosinusitis With Nasal Polyposis
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Background and Objectives A recent study revealed that calcitonin gene-related protein(CGRP) plays an important role in inflammatory airway diseases. However, the influence ofCGRP on chronic rhinosinusitis (CRS) has not been studied. This study investigated the expression,activity, and potential pathogenic role of CGRP in patients with CRS with nasal polyposis(CRSwNP). Subjects and Method Patients with CRSwNP and control subjects were enrolled. TheCRSwNP group was divided according to the presence of eosinophilic polyps and non-eosinophilicpolyps. Nasal polyps (NPs) and uncinate tissues (UTs) from patients with CRSwNPand UTs from control subjects were obtained to investigate the expression of α-/β-CGRP andchromogranin A. In addition, the expression patterns of cytokines following exposure to exogenousCGRP were analyzed in dispersed nasal polyp cells (DNPCs) from patients with eosinophilicor non-eosinophilic CRSwNP. The effects of CGRP on lipopolysaccharide (LPS)-induced nuclear factor-kappa light chain enhancer of activated B cells (NF-κB) signalingchange were evaluated in THP-1 cells. Results The expression of α-/β-CGRP and number of CGRP-producing cells were significantlyhigher in NPs from patients with CRSwNP than in UTs from controls. Exogenous CGRPdecreased the expression of inflammatory cytokines and increased that of the anti-inflammatorycytokines in DNPCs from patients with eosinophilic nasal polyps (EPs) and also increasedthe expression of tissue remodeling-related and anti-inflammatory cytokines in DNPCs frompatients with non-eosinophilic nasal polyps (N-EPs). CGRP inhibited the nuclear factor of kappalight polypeptide gene enhancer in B-cells inhibitor (IκB) phosphorylation and NF-κB translocationin LPS-stimulated M1 macrophages. Conclusion CGRP expression in NPs may play a significant role in nasal polypogenesisthrough inflammatory modulation, and it could be a future target to modulate certain aspectsof CRSwNP.
Abstract
Background and Objectives A recent study revealed that calcitonin gene-related protein(CGRP) plays an important role in inflammatory airway diseases. However, the influence ofCGRP on chronic rhinosinusitis (CRS) has not been studied. This study investigated the expression,activity, and potential pathogenic role of CGRP in patients with CRS with nasal polyposis(CRSwNP). Subjects and Method Patients with CRSwNP and control subjects were enrolled. TheCRSwNP group was divided according to the presence of eosinophilic polyps and non-eosinophilicpolyps. Nasal polyps (NPs) and uncinate tissues (UTs) from patients with CRSwNPand UTs from control subjects were obtained to investigate the expression of α-/β-CGRP andchromogranin A. In addition, the expression patterns of cytokines following exposure to exogenousCGRP were analyzed in dispersed nasal polyp cells (DNPCs) from patients with eosinophilicor non-eosinophilic CRSwNP. The effects of CGRP on lipopolysaccharide (LPS)-induced nuclear factor-kappa light chain enhancer of activated B cells (NF-κB) signalingchange were evaluated in THP-1 cells. Results The expression of α-/β-CGRP and number of CGRP-producing cells were significantlyhigher in NPs from patients with CRSwNP than in UTs from controls. Exogenous CGRPdecreased the expression of inflammatory cytokines and increased that of the anti-inflammatorycytokines in DNPCs from patients with eosinophilic nasal polyps (EPs) and also increasedthe expression of tissue remodeling-related and anti-inflammatory cytokines in DNPCs frompatients with non-eosinophilic nasal polyps (N-EPs). CGRP inhibited the nuclear factor of kappalight polypeptide gene enhancer in B-cells inhibitor (IκB) phosphorylation and NF-κB translocationin LPS-stimulated M1 macrophages. Conclusion CGRP expression in NPs may play a significant role in nasal polypogenesisthrough inflammatory modulation, and it could be a future target to modulate certain aspectsof CRSwNP.
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