Therapeutic Efficacy of Bone Marrow Derived Mesenchymal Stem Cells in Ototoxic Sensorineural Hearing Loss
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Background and Objectives Ototoxic sensorineural hearing loss causes permanent hearingloss in most cases. Recently there have been many reports describing cell base therapywith stem cells that has some effect on hearing recovery. We evaluated the efficacy of clinicalgrade, pre-made, human bone marrow derived mesenchymal stem cells (BM-MSCs) in ototoxicdeaf animal model. Materials and Method BM-MSCs were cultured in a clinical grade laboratory. The animalswere divided into 2 groups as follows: a saline injected control group and a stem cell injectedgroup (MSC-group). Cultured MSCs were transplanted into the brachial vein of the deaf micemodel. We recorded auditory brainstem response (ABR) and conducted immunohistochemistryat 1, 3, and 5 weeks. Results After the transplantation of MSC, a significant improvement in the hearing thresholdof ABR was observed in the MSC transplanted group. Five weeks after transplantation ofMSCs, hair cell regeneration was confirmed from the basal to the apex of the cochlea in fluorescentdyed image under the microscope compared to the control group. Conclusion BM-MSCs were effective in an acute ototoxic deaf animal model. These resultsshow that stem cell transplantation mediate inner ear regeneration.
Abstract
Background and Objectives Ototoxic sensorineural hearing loss causes permanent hearingloss in most cases. Recently there have been many reports describing cell base therapywith stem cells that has some effect on hearing recovery. We evaluated the efficacy of clinicalgrade, pre-made, human bone marrow derived mesenchymal stem cells (BM-MSCs) in ototoxicdeaf animal model. Materials and Method BM-MSCs were cultured in a clinical grade laboratory. The animalswere divided into 2 groups as follows: a saline injected control group and a stem cell injectedgroup (MSC-group). Cultured MSCs were transplanted into the brachial vein of the deaf micemodel. We recorded auditory brainstem response (ABR) and conducted immunohistochemistryat 1, 3, and 5 weeks. Results After the transplantation of MSC, a significant improvement in the hearing thresholdof ABR was observed in the MSC transplanted group. Five weeks after transplantation ofMSCs, hair cell regeneration was confirmed from the basal to the apex of the cochlea in fluorescentdyed image under the microscope compared to the control group. Conclusion BM-MSCs were effective in an acute ototoxic deaf animal model. These resultsshow that stem cell transplantation mediate inner ear regeneration.
참고문헌 (18)
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