치료 중인 만성 B형 간염 한국인 환자의 B형 간염 바이러스 유전자아형 및 혈청형 분석
Subgenotype and Serotype Analysis of Hepatitis B virus in Korean Chronic Hepatitis B Patients Under Treatment
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배경 : 한국인 환자에서 검출되는 B형 간염 바이러스(HBV) 는 대부분 C형 유전자형이며, 이는 C1 (혹은 Cs)형과 C2 (혹은 Ce)형 유전자아형으로 구별된다. 최근 C1 및 C2 유전자아형 사 이에 간암 위험도에 차이를 보인다는 보고가 있었다. 이에 저자 들은 한국인 만성 B형 간염 환자를 대상으로 HBV 유전자아형 의 분포를 알아보고자 하였다. 방법 : 울산의대 서울아산병원에 만성 B형 간염으로 진단받 고 치료 중인 421명의 환자 검체를 대상으로 하였다. 직접염기 서열법으로 HBV S 유전자 부위를 분석한 후 계통발생학적 방 법으로 유전자아형을 결정하였다. 동일자료를 이용하여 혈청형 의 분포를 아울러 조사하였다. 결과 : 421명의 환자 검체에서 C형 유전자형은 419명(99.5%), B형은 2명(0.5%)이었다. C형 유전자형 중 단독 417명은 C2 (Ce)형 유전자아형이었고, 나머지 2명은 혼합 염기서열을 보였 다. 그러나, 혼합 염기서열 내에서도 모두 C2형의 바이러스 주 가 분명히 관찰되는 혼합 염기서열을 보였다. C형 유전자형인 419명의 혈청형은 adr 385명(91.9 %), adw 22명(5.3%), ayr 2 명(0.4%), 혼합형 10명(2.3%)이었다. B형 유전자형인 2명은 모 두 adw 혈청형이었다. 결론 : 치료 중인 만성B형간염 한국인 환자에서 검출되는 HBV는 거의 대부분 C2 (Ce)형임을 알 수 있었다.
Abstract
Background : Hepatitis B virus (HBV) detected in Korean patients almost belongs to genotype C, which is subdivided into subgenotype C1 (or Cs) and C2 (or Ce). It was recently reported that the risk of hepatocellular carcinoma is different between subgenotype C1 and C2. Thus, we studied the distribution of subgenotypes of HBV in Korean chronic hepatitis B (CHB) patients. Methods : Specimens of 421 patients, who were diagnosed as CHB and underwent antiviral treatment, were used. After sequence analysis for HBV S gene, subgenotype was identified through phylogenetic analysis. Utilizing the same sequence data, the distribution of serotypes was also investigated. Results : Among 421 patient specimens, genotype C was found in 419 (99.5%) and genotype B in 2 (0.5%). Among the genotype C strains, 417 strains were C2 subgenotype and 2 strains were mixed subgenotypes. However, C2 was evidently found even in the mixed sequences. Serotypes of 419 HBV with genotype C were classified as follows: adr, 385 (91.9%), adw, 22 (5.3%), ayr, 2 (0.4%) and mixed serotype, 10 (2.3%). Serotype of both HBV with genotype B was adw. Conclusions : It was found that HBV detected in Korean CHB patients under treatment almost all belong to the C2 (Ce) genotype.
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