기증된 한국인 신생아 제대혈의 전혈구 수(complete blood count) 참고치
Complete Blood Count Reference Values of Donated Cord Blood from Korean Neonates
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초록
배경 : 공여제대혈은행에서는 세포 수가 충분하고 안전한 제 대혈에 대해서만 저장 처리를 시행하는데 제대혈 전혈구 수 검사 는 제대혈의 총 유핵세포 수를 산정하고 혈액학적 이상이 없는 이식적합 제대혈을 선별하는데 중요한 검사이다. 본 연구에서는 공여제대혈은행에 기증 및 저장된 제대혈 검체를 대상으로 건강 한 한국인 신생아 제대혈의 전혈구 수에 대한 참고치를 설정하 고자 하였다. 방법 : 2007년 8월 1일부터 12월 31일까지 기증 후 저장 처리 된 제대혈 검체 2,129단위를 대상으로 XE-2100 자동혈구분석 기(Sysmex, Japan)를 이용하여 혈색소, 백혈구 수, 백혈구 감별계수, 혈소판 수 및 유핵적혈구 수를 측정하고. 각 항목별로 모수법(Mean±2SD) 또는 비모수법(2.5-97.5 백분위수)으로 참고치를 설정하였다. 이와 함께, 신생아 성별 및 분만 방법에 따른 전혈구 수 항목별 측정값을 비교하였다. 결과 : 하위 그룹 간 전혈구 수 결과의 차이가 근소하여 그룹 별 참고치 구분은 필요하지 않았으며, 제대혈 전혈구 수 참고치 를 다음과 같이 설정하였다: 혈색소, 9.0-14.4 g/dL; 백혈구 수, 5.6-18.5×103/μL; 백혈구 감별계수(호중구, 40.8-72.4%; 림 프구, 17.2-46.7%; 단구, 4.9-12.8%; 호산구, 0.7-7.0%; 호염구, 0.0-1.6%); 혈소판 수, 130-287×103/μL; 유핵적혈구 수, 0.0- 13.1/100 WBCs. 결론 : 저자들은 대단위의 제대혈 검체를 대상으로 정상 한국 인 신생아의 제대혈 전혈구 수 참고치를 설정하였으며, 본 연구 를 통해 설정된 참고치는 향후 제대혈은행에 기증되는 제대혈의 검사 결과 해석과 저장 적합성 평가에 있어서 기초 자료로 유용 하게 이용될 것이다.
Abstract
Background : In the public cord blood (CB) banks, only safe CB units with adequate cell doses are processed and stored. Complete blood count (CBC) of CB is crucial for estimating total nucleated cells (TNC) and screening suitable CB units without hematologic abnormalities. We analyzed CBC parameters of the donated CB from healthy Korean neonates to establish CBC reference values. Methods : A total of 2,129 Korean CB units, donated and processed during the period from August 2007 to December 2007, were enrolled. We measured hemoglobin (Hb), white blood cell (WBC) count, differential count of WBC, platelets and nucleated red blood cell (nRBC) count by XE-2100 automated hematology analyzer (Sysmex, Japan), and estimated reference value of each parameter by using parametric (Mean±2SD) and/or non-parametric methods (2.5-97.5 percentile). And also, we compared the result of each parameter in relation to sex of neonates and delivery method. Results : Because the differences of CBC values among different subgroups were not remarkable, we established the reference intervals as follows without subgroup division: Hb, 9.0-14.4 g/dL; WBC count, 5.6-18.5×103/μL; differential count of WBC (neutrophils, 40.8-72.4%; lymphocytes, 17.2-46.7%; monocytes, 4.9-12.8%; eosinophils, 0.7-7.0%; basophils, 0.0-1.6%); platelet, 130-287×103/μL; nRBCs, 0.0-13.1/100 WBC. Conclusions : We established cord blood CBC reference values of healthy Korean neonates using a large-scale CB units. The established CBC reference values from our study will be useful as basic data for CBC interpretation and assessment of transplant suitability of donated CB.
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