The Evaluation of SD Bioline Strep A Rapid Antigen Test in Acute Pharyngitis in Pediatric Clinics
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Background : Acute pharyngitis is a very common respiratory tract infection. Rapid antigen tests (RATs) that detect group A streptococci (GAS) have an advantage over conventional throat culture in determining the cause of acute pharyngitis quickly. The efficiency of RAT should be good enough to be used in the laboratory or in the clinics. Methods : From October 2008 through February 2009, throat swabs were taken from 293 children with acute pharyngitis and conveyed to the Gyeongsang National University Hospital in a transport medium. Two swabs from each patient were inoculated onto a blood agar plate, then returned to the transport medium and stored at -20℃ for several months. After the samples were thawed at room temperature, the SD Bioline Strep A RAT (SD, Korea) was performed. Results : The sensitivity, specificity, positive predictive value, and negative predictive value of SD Bioline Strep A compared with throat culture were 95.9% (95% confidence interval [CI]: 93.6-98.2%), 91.8% (95% CI: 88.5-95.1%), 95.9% (95% CI: 93.6-98.2%), and 91.8% (95% CI: 88.5-95.1%), respectively. Conclusions : The SD Bioline Strep A RAT kit can be useful as an alternative to throat cultures in the clinics for rapid diagnosis of GAS pharyngitis and for an early decision on the use of antibiotics.
Abstract
Background : Acute pharyngitis is a very common respiratory tract infection. Rapid antigen tests (RATs) that detect group A streptococci (GAS) have an advantage over conventional throat culture in determining the cause of acute pharyngitis quickly. The efficiency of RAT should be good enough to be used in the laboratory or in the clinics. Methods : From October 2008 through February 2009, throat swabs were taken from 293 children with acute pharyngitis and conveyed to the Gyeongsang National University Hospital in a transport medium. Two swabs from each patient were inoculated onto a blood agar plate, then returned to the transport medium and stored at -20℃ for several months. After the samples were thawed at room temperature, the SD Bioline Strep A RAT (SD, Korea) was performed. Results : The sensitivity, specificity, positive predictive value, and negative predictive value of SD Bioline Strep A compared with throat culture were 95.9% (95% confidence interval [CI]: 93.6-98.2%), 91.8% (95% CI: 88.5-95.1%), 95.9% (95% CI: 93.6-98.2%), and 91.8% (95% CI: 88.5-95.1%), respectively. Conclusions : The SD Bioline Strep A RAT kit can be useful as an alternative to throat cultures in the clinics for rapid diagnosis of GAS pharyngitis and for an early decision on the use of antibiotics.
참고문헌 (12)
- Centor RM, "The diagnosis of strep throat in adults in the emergency room", Med Decis Making, 1, pp.239~246 (1981)
- Bisno AL, "Practice guidelines for the diagnosis and management of group A streptococcal pharyngitis. Infectious Diseases Society of America", Clin Infect Dis, 35, pp.113~125 (2002)
- American Academy of Pediatrics, "Group A streptococcal infections, in The Red Book. 25th ed", pp.526~536 (2000), American Academy of Pediatrics
- Gerber MA, "Rapid diagnosis of pharyngitis caused by group A streptococci", Clin Microbiol Rev, 17, pp.571~580 (2004)
- Nerbrand C, "Are current rapid detection tests for group A streptococci sensitive enough? Evaluation of 2 commercial kits", Scand J Infect Dis, 34, pp.797~799 (2002)
- Kellogg JA, "Suitability of throat culture procedures for detection of group A streptococci and as reference standards for evaluation of streptococcal antigen detection kits", J Clin Microbiol, 28, pp.165~169 (1990)
- 박수진, "진주지역 초등학생에서 분리된 A군 연쇄구균의 항생제 내성 양상 및 내성 기전", 대한임상미생물학회지, 6, pp.7~11 (2003)
- Rubin LG, "Apparent false positive detection of group A Streptococcus antigen resulting from pharyngeal infection with a nonhemolytic Streptococcus pyogenes", Pediatr Infect Dis J, 19, pp.672~674 (2000)
- Johnson DR, "False-positive rapid antigen detection test results: reduced specificity in the absence of group A streptococci in the upper respiratory tract", J Infect Dis, 183, pp.1135~1137 (2001)
- 고은하, "초등학생에서 세균성 인두염 신속항원검사 SD Bioline Strep A의 평가", 대한임상미생물학회지, 10(1), pp.54~58 (2007), 대한임상미생물학회
- Gieseker KE, "Evaluating the American Academy of Pediatrics diagnostic standard for Streptococcus pyogenes pharyngitis: backup culture versus repeat rapid antigen testing", Pediatrics, 111, pp.e666~e670 (2003)
- Fox JW, "Performance of rapid streptococcal antigen testing varies by personnel", J Clin Microbiol, 44, pp.3918~3922 (2006)