돼지 육골분 및 진주담치 단백질의 가수분해물 제조 및 칼슘 결합 물질의 분리
Isolation of calcium-binding peptides from porcine meat and bone meal and mussel protein hydrolysates
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초록
저활용 단백질로부터 칼슘 결합물질을 분리하기 위해 돼지 육골분과 진주담치 단백질을 단백질 분해 효소인 alcalase를 이용하여 가수분해물을 제조하였고, 체내 흡수가 용이한 3 kDa 이하로 한외여과 하였다. 돼지 육골분 가수분해물은 Mono Q 컬럼을 통해 분리하였고, 진주담치 가수분해물의 경우 Q-Sepharose로 분리 하여 각각 2개, 3개의 peptide fraction을 얻어 각 fraction의 칼슘 결합력을 측정하였다. 그 결과 MBM F2와 Mussel F3에서 가장 높은 칼슘 결합력을 나타내었고, 따라서 본 연구 결과로 얻어진 가수분해물들은 칼슘 보충 소재로 활용될 수 있다고 판단된다.
Abstract
Calcium is one of the essential mineral for the humans due to its crucial physiological functions in the body. Calcium deficiency results in many diseases, such as osteoporosis. Therefore, calcium supplements are available as a functional food. However, most calcium supplements in the market have a limitation due to poor absorption and low bioavailability. Thus, calcium-chelated peptides for improving the absorption rate of calcium have been isolated from foods including porcine meat and bone meal (MBM), and mussel using the enzymatic hydrolysis of their protein. The hydrolysates of food were ultra-filtered in order to obtain small peptides less than 3 kDa and the Ca-binding peptides were isolated via the anion exchange chromatography. The binding activity and concentration of Ca-binding pepetides were determined. In particular, the MBM and mussel protein hydrolysates were fractionated by mono Q and Q-Sepharose, respectively. As a result, among the fractions, the fractions of MBM F2 and mussel F3 showed the highest Ca-binding activity. These results suggest that MBM and mussel protein hydrolysates can be used as calcium supplements.
참고문헌 (31)
- Bass JK, "Calcium nutrition and metabolism during infancy", Nutr, 22, pp.1057~1066 (2006)
- Barclay JW, "Calcium-dependent regulation of exocytosis", Cell Calcium, 38, pp.343~353 (2005)
- Miquel E, "Effects and future trends of casein phosphopeptides on zinc bioavailability", Trends Food Sci Tech, 18, pp.139~143 (2007)
- Poitou Bernert C, "Nutritional deficiency after gastric bypass : diagnosis, prevention and treatment", Diabetes Metab, 33, pp.13~24 (2007)
- Guo L, "Food protein-derived chelating peptides : biofunctional ingredients for dietary mineral bioavailability enhancement", Trends Food Sci Tech, 37, pp.92~105 (2014)
- Bolarinwa OA, "Metabolizable energy value of porcine meat and bone meal for broiler chickens", Can J Anim Sci, 92, pp.73~78 (2012)
- 김선근, "자연산 홍합(Mytilus coruscus)과 양식산 진주담치(Mytilus edulis)의성분 특성", 한국수산과학회지, 46(6), pp.717~724 (2013), 한국수산과학회
- Cha YJ, "Flavor and taste-active compounds in blue mussel hydrolysate produced by protease", J Food Sci Nutr, 3, pp.15~21 (1998)
- Garcia RA, "Physical distribution and characteristics of meat and bone meal protein", J Sci Food Agr, 89, pp.329~336 (2009)
- Wang B, "Purification and characterisation of a novel antioxidant peptide derived from blue mussel(Mytilus edulis)protein hydrolysate", Food Chem, 138, pp.1713~1719 (2013)
- Vareltzis PK, "Protein isolation from blue mussels(Mytilus edulis)using an acid and alkaline solubilisation technique-tprocess characteristics and functionality of the isolates", J Sci Food Agr, 92, pp.3055~3064 (2012)
- 김남호, "Purification of an Iron-Chelating Peptide from Spirulina Protein Hydrolysates", Applied Biological Chemistry, 57(1), pp.91~95 (2014), 한국응용생명화학회
- Ekund A, "On the determination of available lysine in casein and rapeseed protein concentration using 2, 4, 6-trinitrobenzenesulfonic acid(TNBS)as a reagent of free αα-amino group of lysine", Anal Chem, 70, pp.434~439 (1976)
- Nielsen PM, "Improved method for determining food protein degree of hydrolysis", J Food Sci, 66, pp.642~646 (2001)
- Wu H, "Enzymatic preparation and characterization of iron-chelating peptides from anchovy(Engraulis japonicus)muscle protein", Food Res Int, 48, pp.435~441 (2012)
- Gitelman HJ, "An improved automated procedure for the determination of calcium in biological specimens", Anal Biochem, 18, pp.521~531 (1967)
- Korhonen H, "Bioactive peptides : production and functionality", Int Dairy J, 16, pp.945~960 (2006)
- Chen D, "Isolation of a calcium-binding peptide from tilapia scale protein hydrolysate and its calcium bioavailability in rats", J Funct Foods, 6, pp.575~584 (2014)
- Charoenphun N, "Calcium-binding peptides derived from tilapia(Oreochromis niloticus)protein hydrolysate", Eur Food Res Technol, 236, pp.57~63 (2013)
- 이지혜, "보리 단백질 가수분해물로부터 칼슘 결합 물질의 분리", 한국식품저장유통학회지, 19(3), pp.438~442 (2012), 한국식품저장유통학회[DOI]
- Mine Y, "Nutraceutical proteins and peptides in health and disease", pp.3~9 (2006), CRP Press
- Jung WK, "Fish-bone peptide increases calcium solubility and bioavailability in ovariectomised rats", Brit J Nutr, 95, pp.124~128 (2006)
- Jung WK, "Recovery of a novel Ca-binding peptide from Alaska pollack(Theragra chalcogramma)backbone by pepsinolytic hydrolysis", Process Biochem, 41, pp.2097~2100 (2006)
- Seung Hwan Lee, "Isolation of a Calcium-binding Peptide from Enzymatic Hydrolysates of Porcine Blood Plasma Protein", Applied Biological Chemistry, 52(3), pp.290~294 (2009), 한국응용생명화학회
- Jung WK, "Calcium-binding peptide derived from pepsinolytic hydrolysates of hoki(Johnius belengerii)frame", Eur Food Res Technol, 224, pp.763~767 (2007)
- Vegarud GE, "Mineral-binding milk proteins and peptides : occurrence, biochemical and technological characteristics", Brit J Nutr, 84, pp.91~98 (2000)
- So Jeong Jeon, "Isolation of a Calcium-binding Peptide from Chlorella Protein Hydrolysates", Preventive Nutrition and Food Science, 15(4), pp.282~286 (2010), 한국식품영양과학회
- Huang G, "Purification of a histidine-containing peptide with calcium binding activity from shrimp processing byproducts hydrolysate", Eur Food Res Technol, 232, pp.281~287 (2011)
- 최동원, "면실박 단백질로부터 가수분해물 제조 및 철분, 칼슘 결합 펩타이드의 분리", Journal of Applied Biological Chemistry, 55(4), pp.263~266 (2012), 한국응용생명화학회
- Parsons CM, "Protein and amino acid quality of meat and bone meal", Poultry Sci, 76, pp.361~368 (1997)
- Silva VM, "Optimization of the enzymatic hydrolysis of mussel meat", J Food Sci, 75, pp.36~42 (2010)