Frequency of FCGR3B Alleles in Thai Blood Donors
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Human neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described in different populations. This study investigated the frequency of FCGR3B alleles encoding HNA-1a, HNA-1b, and HNA-1c among Thai blood donors and compared these frequencies with those previously reported for other populations. Methods: Eight hundred DNA samples obtained from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, and the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, were included. Samples were simultaneously typed for each FCGR3B allele using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. Results: The frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004, respectively; only FCGR3B*1 and FCGR3B*2 alleles were found in northern Thai blood donors (0.68 and 0.32, respectively). Compared with other Asian populations, central Thais had higher frequencies of the 2 allele (P<0.001), while the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in northern Thais were similar to those previously reported in Taiwanese and Japanese populations. In contrast, the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in the northern Thai population were statistically different from those observed in central Thai, Korean, German, and Turkish populations. Conclusions: FCGR3B allele frequencies were significantly different between central and northern Thai blood donors. Our in-house PCR-SSP method is a simple, cost-effective, and convenient method for FCGR3B allele detection.
Abstract
Background: Human neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described in different populations. This study investigated the frequency of FCGR3B alleles encoding HNA-1a, HNA-1b, and HNA-1c among Thai blood donors and compared these frequencies with those previously reported for other populations. Methods: Eight hundred DNA samples obtained from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, and the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, were included. Samples were simultaneously typed for each FCGR3B allele using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. Results: The frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004, respectively; only FCGR3B*1 and FCGR3B*2 alleles were found in northern Thai blood donors (0.68 and 0.32, respectively). Compared with other Asian populations, central Thais had higher frequencies of the 2 allele (P<0.001), while the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in northern Thais were similar to those previously reported in Taiwanese and Japanese populations. In contrast, the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in the northern Thai population were statistically different from those observed in central Thai, Korean, German, and Turkish populations. Conclusions: FCGR3B allele frequencies were significantly different between central and northern Thai blood donors. Our in-house PCR-SSP method is a simple, cost-effective, and convenient method for FCGR3B allele detection.
참고문헌 (22)
- Stephens HA, "A comparison of molecular HLA-DR and DQ allele profiles forming DR51-, DR52-, and DR53-related haplotypes in five ethnic Thai populations from mainland southeast Asia", Hum Immunol, 61, pp.1039~1047 (2000)
- Chu JY, "Genetic relationship of populations in China", Proc Natl Acad Sci USA, 95, pp.11763~11768 (1998)
- Piazza A, "Towards a genetic history of China", Nature, 395, pp.636~637 (1998)
- de La Vega Elena CD, "HNA-1a, HNA-1b and HNA-1c gene frequencies in Argentineans", Tissue Antigens, 71, pp.475~477 (2008)
- Bux J, "Human neutrophil alloantigens", Vox Sang, 94, pp.277~285 (2008)
- Xia W, "The frequencies of human neutrophil alloantigens in the Chinese Han population of Guangzhou", Transfusion, 51, pp.1271~1277 (2011)
- Chu CC, "The use of genotyping to predict the phenotypes of human platelet antigens 1 through 5 and of neutrophil antigens in Taiwan", Transfusion, 41, pp.1553~1558 (2001)
- Bux J, "Characterization of a new alloantigen (SH) on the human neutrophil Fc gamma receptor IIIb", Blood, 89, pp.1027~1034 (1997)
- Kissel K, "HNA-1a, HNA-1b, and HNA-1c (NA1, NA2, SH) frequencies in African and American Blacks and in Chinese", Tissue Antigens, 56, pp.143~148 (2000)
- Bux J, "Serological and clinical aspects of granulocyte antibodies leading to alloimmune neonatal neotropenia", Transfus Med, 2, pp.143~149 (1992)
- Lalezari P, "Chronic autoimmune neutropenia due to anti-NA2 antibody", N Engl J Med, 293, pp.744~747 (1975)
- Yomtovian R, "Severe pulmonary hypersensitivity associated with passive transfusion of a neutrophil-specific antibody", Lancet, 1, pp.244~246 (1984)
- Covas DT, "Variation in the FcγR3B gene among distinct Brazilian populations", Tissue Antigens, 65, pp.178~182 (2005)
- Fujiwara K, "Determination of granulocyte-specific antigens on neutrophil FcA receptor IIIb by PCR-preferential homoduplex formation assay, and gene frequencies in the Japanese population", Vox Sang, 77, pp.218~222 (1999)
- Kuwano ST, "Allelic polymorphisms of human fcgamma receptor IIa and Fcgamma receptor IIIb among distinct groups in Brazil", Transfusion, 40, pp.1388~1392 (2000)
- Hassine MO, "FCGR3B allele frequencies in Tunisians of sub-Saharan origin", Transfus Clin Biol, 19, pp.60~63 (2012)
- Changsri K, "Human neutrophil alloantigen genotype frequencies in Thai blood donors", Blood Transfus, 23, pp.1~6 (2013)[DOI]
- Miller AS, "A simple salting out procedure for extracting DNA for human nucleated cells", Nucleic Acids Res, 16, pp.1215~ (1988)
- Steffensen R, "FcγRIIIB polymorphism: evidence that NA1", NA2 and SH are located in two closely linked loci and that the SH allele is linked to the NA1 allele in the Danish population, 1999, pp.39, 593~598
- Seo DH, "Genotype analysis of granulocyte-specific antigens in Koreans", Korean J Clin Pathol, 17, pp.1144~1149 (1997)
- Hauck B, "Human neutrophil alloantigen genotype frequencies among blood donors with Turkish and German descent", Tissue Antigens, 78, pp.416~420 (2011)
- Muschter S, "Developments in the definition and clinical impact of human neutrophil antigens", Curr Opin Hematol, 18, pp.452~460 (2011)