혈관내피세포에서 TNF-α 자극에 의해 유도되는 혈관염증에 대한 전통식품 품질인증 된장의 효능 평가
Protective effects of quality certified traditional Doenjang in Korea on TNF-α-induced vascular inflammation in human umbilical vein endothelial cells
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Anti-atherogenic effects in tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells (HUVEC) are involved in the suppression of oxidative stress, cell adhesion molecules, and pro-inflammatory factors. This study investigated the vascular inflammation inhibitory activity of traditional Doenjang plays a key role in the pathogenesis and progression of atherosclerosis. The protective effects of Korean Deonjang was investigated on the expression of cell adhesion molecules (CAMs) in tumor necrosis factor (TNF)-α-induced human umbilical vascular endothelial cells (HUVECs). Deonjang extracts (20, 50, 100 μg/mL) decreased the expression of 20 ng/mL TNF-α-induced vascular cell adhesion molecule (VCAM)-1 intracellular adhesion molecule (ICAM)-1 proteins, and their corresponding mRNA levels. Nitric oxides (NO) produced by endothlial nitric oxides synthase (eNOS) dilated blood vessels, which had protective effects against platelet and leukocyte adhesion. While TNF-α-induced suppressed the production of nitric oxide in HUVECs, Doenjang restored NO production in HUVECs. In addition, Deonjang reduced the TNF-α-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA levels. These results suggested that Doenjang can inhibited the production of cell adhesion molecules and inflammatory mediators, which could be a potential candidate for preventing atherosclerosis.
Abstract
Anti-atherogenic effects in tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells (HUVEC) are involved in the suppression of oxidative stress, cell adhesion molecules, and pro-inflammatory factors. This study investigated the vascular inflammation inhibitory activity of traditional Doenjang plays a key role in the pathogenesis and progression of atherosclerosis. The protective effects of Korean Deonjang was investigated on the expression of cell adhesion molecules (CAMs) in tumor necrosis factor (TNF)-α-induced human umbilical vascular endothelial cells (HUVECs). Deonjang extracts (20, 50, 100 μg/mL) decreased the expression of 20 ng/mL TNF-α-induced vascular cell adhesion molecule (VCAM)-1 intracellular adhesion molecule (ICAM)-1 proteins, and their corresponding mRNA levels. Nitric oxides (NO) produced by endothlial nitric oxides synthase (eNOS) dilated blood vessels, which had protective effects against platelet and leukocyte adhesion. While TNF-α-induced suppressed the production of nitric oxide in HUVECs, Doenjang restored NO production in HUVECs. In addition, Deonjang reduced the TNF-α-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA levels. These results suggested that Doenjang can inhibited the production of cell adhesion molecules and inflammatory mediators, which could be a potential candidate for preventing atherosclerosis.
참고문헌 (41)
- 임선영, "된장 메탄올 추출물의 인체 암세포 성장 억제 효과 및 DNA 합성 저해 효과", 한국식품영양과학회지, 33(6), pp.936~940 (2004), 한국식품영양과학회
- 임선영, "된장 분획물의 항돌연변이 및 암세포 증식 억제효과와 interleukin-2 생성에 미치는 영향", 생명과학회지, 17(6), pp.791~797 (2007), 한국생명과학회
- Kim MJ, "Studies on the changes of taste compounds during soy paste fermentation", Korean J Food Cook Sci, 6, pp.1~8 (1990)
- Coward L, "Genistein, daidzein, and their β-glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets", J Agric Food Chem, 41, pp.1961~1967 (1993)
- Hamalainen M, "Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-κB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-κB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages", Mediators Inflamm, 2007, pp.45673~ (2007)
- Ungar Y, "Thermal stability of genistein and daidzein and its effect on their antioxidant activity", J Agric Food Chem, 51, pp.4394~4399 (2003)
- Guo JM, "Biphasic effect of daidzein on cell growth of human colon cancer cells", Food Chem Toxicol, 42, pp.1641~1646 (2004)
- Park KY, "Antimutagenic effect of Doejang(Korean fermented soy paste)toward aflatoxin", J Korean Soc Food Sci Nutr, 19, pp.158~162 (1990)
- 최승필, "다시마 분말을 첨가한 전통된장 에탄올 추출물의 항돌연변이성 및 항암효과", 한국식품영양과학회지, 31(2), pp.322~328 (2002), 한국식품영양과학회
- Kim SH, "New trends of studying on potential activities of Doenjang-fibrinolytic activity", Korea Soybean Digest, 15, pp.8~15 (1998)
- Lee BK, "Immunomodulators extracted from Korean-style fermented soybean paste and their function. Isolation of B cell mitogen from Korean-style fermented soybean paste", Korean J Immonol, 19, pp.559~569 (1997)
- Suh HJ, "Purification of ACE inhibitor from soybean paste", Appl Biol Chem, 37, pp.441~446 (1994)
- Hwang JH, "Angiotensin I converting enzyme inhibitory effect of Doenjang fermented by B. subtilis SCB-3 isolated from Meju, Korean traditional food", J Koeran Soc Food Sci Nutr, 26, pp.775~783 (1997)
- Yang BK, "Hypoglycemic effect of extracts of soybean paste containing mycelia of mushrooms in streptozotocin-induced diabetic rats", Korean J Mycol, 28, pp.126~129 (2000)
- Choe GS, "Antioxidant and nitrite scavenging effect of soybean, Meju and Doenjang", Korean J Life Sci, 8, pp.473~478 (1998)
- Kim MH, "Antioxidative materials in domestic Meju and Doenjang 2. Separation of lipophilic brown pigment and their antioxidative activity", J Korean Soc Food Nutr, 23, pp.251~260 (1994)
- Choi UK, "Optimum condition for pigment production and antioxidative activity of the products by Bacillus subtilis DC-2 with response surface methodology", J Korean Soc Food Sci Nutr, 26, pp.620~624 (1997)
- Ross R, "Cell biology of atherosclerosis", Annu Rev Physiol, 57, pp.791~804 (1995)
- Lee ES, "Inhibition of nitric oxide and tumor necrosis factor-α(TNF-α)production by propenone compound through blockade of nuclear factor(NF)-κB activation in cultured murine macrophages", Biol Pharm Bull, 27, pp.617~620 (2004)
- Tae Hoon Lee, "Methanol Extracts of Stewartia koreana Inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by Blocking NF-κB Transactivation in LPS-activated RAW 264.7 Cells", Molecules and Cells, 23(3), pp.398~404 (2007), 한국분자세포생물학회
- Nathan C, "Nitric oxide as a secretory product of mammalian cells", FASEB J, 6, pp.3051~3064 (1992)
- Mohamed AK, "The role of oxidative stress and NF-κB activation in late diabetic complications", Biofactors, 10, pp.157~167 (1999)
- Chen JW, "Ginkgo biloba extract inhibits tumor necrosis factor-α-induced reactive oxygen species generation, transcription factor activation, and cell adhesion molecule expression in human aortic endothelial cells", Arterioscler Thromb Vasc Biol, 23, pp.1559~1566 (2003)
- Kanters E, "Inhibition of NF-κB activation in macrophages increases atherosclerosis in LDL receptor-deficient mice", J Clin Invest, 112, pp.1176~1185 (2003)
- Kunsch C, "Selective inhibition of endothelial and monocyte redox-sensitive genes by AGI-1067: a novel antioxidant and anti-inflammatory agent", J Pharmacol Exp Ther, 308, pp.820~829 (2004)
- 허정인, "농식품 인증 및 전통식품 품질인증에 관한 인식조사", 한국식생활문화학회지, 26(3), pp.220~229 (2011), 한국식생활문화학회
- National Agricultural Products Quality Management Service, "Traditional Food Standards", 88~100 (2012)
- 강지은, "전통식품 품질인증 된장의 품질특성", 한국지역사회생활과학회지, 24(4), pp.537~542 (2013), 한국지역사회생활과학회
- Je JY, "Antioxidant and angiotensin I converting enzyme inhibitory activity of Bambusae caulis in liquamen", Food Chem, 113, pp.932~935 (2009)
- Lee SJ, "Purification and characterization of a nitric oxide inhibitory peptide from Ruditapes philippinarum", Food Chem Toxicol, 50, pp.1660~1666 (2012)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
- Kim JY, "Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages", FEBS Lett, 569, pp.321~326 (2004)
- Li H, "Nitric oxide in the pathogenesis of vascular disease", J Pathol, 190, pp.244~254 (2000)
- Sarkar D, "Molecular mechanisms of aging-associated inflammation", Cancer Lett, 236, pp.13~23 (2006)
- Vodovotz Y, "Vesicle membrane association of nitric oxide synthase in primary mouse macrophage", J Immunol, 154, pp.2914~2925 (1995)
- 성미선, "RAW264.7 대식세포에서 Heme Oxygenase-1의 유도에 의한 개망초 (Erigeron annuus L.) 꽃 Methanol 추출물의 항염증 효과", 한국식품영양과학회지, 40(11), pp.1507~1511 (2011), 한국식품영양과학회
- 박수영, "큰비쑥(Artemisia fukudo) 추출물의 murine macrophage RAW", 한국식품과학회지, 39(4), pp.464~469 (2007), 한국식품과학회
- Hwang SJ, "Circulating adhesion molecules VCAM-1, ICAM-1, and E-selectin in carotid atherosclerosis and incident coronary heart disease cases", Circulation, 96, pp.4219~4225 (1997)
- Li H, "An atherogenic diet rapidly induces VCAM-1, a cytokine-regulatable mononuclear leukocyte adhesion molecule, in rabbit aortic endothelium", Arterioscler Thromb, 13, pp.197~204 (1993)
- Schmidt A, "Lovastatin-stimulated superinduction of E-selectin, ICAM-1 and VCAM-1 in TNF-α activated human vascular endothelial cells", Atherosclerosis, 164, pp.57~64 (2002)
- Rimbach G, "Macrophages stimulated with IFN-γ activate NF-κB and induce MCP-1 gene expression in primary human endothelial cells", Mol Cell Biol Res Commun, 3, pp.238~242 (2000)
