Evaluation of a Novel Array-Based Toxoplasma, Rubella, Cytomegalovirus, and Herpes Simplex Virus IgG Enzyme Linked Immunosorbent Assay and Its Comparison with Virion/Serion Enzyme Linked Immunosorbent Assays
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: The dramatic increase in use of the IgG test for toxoplasma, rubella, cyto- megalovirus (CMV), and herpes simplex virus (HSV) [TORCH] has led to the requirement for a high-efficiency method that can be used in the clinical laboratory. This study aimed to compare the results of BGI-Array ELISA TORCH IgG (BGI-GBI, China) screening method to those of Virion/Serion TORCH IgG ELISA (Virion/Serion, Germany). Methods: Serum specimens (n=400) submitted for routine IgG testing by Virion/Serion ELISA were also tested using the BGI-Array ELISA method. The agreements of these two kinds of method were analyzed by κ-coefficients calculation. Results: Following repeat testing, the BGI-Array ELISA TORCH IgG assays demonstrated agreements of 99.5% (398/400 specimens), 98% (392/400 specimens), 99% (396/400 specimens), and 99.5% (398/400 specimens), respectively. The BGI-Array ELISA IgG as- says provided results comparable to Virion/Serion ELISA results, with κ-coefficients show- ing near-perfect agreement for the HSV (κ=0.87), rubella (κ=0.92) and CMV (κ=0.93) and substantial agreement for the toxoplasma (κ=0.80) IgG assays. The use of the BGI- Array ELISA TORCH IgG assays could reduce the turnaround time (1.5 hr vs. 5 hr by Vi- rion/Serion ELISA for 100 specimens) and were easy to use. Conclusions: BGI-Array ELISA TORCH IgG shows a good agreement with Virion/Serion ELISA methods and is suitable for clinical application.
Abstract
Background: The dramatic increase in use of the IgG test for toxoplasma, rubella, cyto- megalovirus (CMV), and herpes simplex virus (HSV) [TORCH] has led to the requirement for a high-efficiency method that can be used in the clinical laboratory. This study aimed to compare the results of BGI-Array ELISA TORCH IgG (BGI-GBI, China) screening method to those of Virion/Serion TORCH IgG ELISA (Virion/Serion, Germany). Methods: Serum specimens (n=400) submitted for routine IgG testing by Virion/Serion ELISA were also tested using the BGI-Array ELISA method. The agreements of these two kinds of method were analyzed by κ-coefficients calculation. Results: Following repeat testing, the BGI-Array ELISA TORCH IgG assays demonstrated agreements of 99.5% (398/400 specimens), 98% (392/400 specimens), 99% (396/400 specimens), and 99.5% (398/400 specimens), respectively. The BGI-Array ELISA IgG as- says provided results comparable to Virion/Serion ELISA results, with κ-coefficients show- ing near-perfect agreement for the HSV (κ=0.87), rubella (κ=0.92) and CMV (κ=0.93) and substantial agreement for the toxoplasma (κ=0.80) IgG assays. The use of the BGI- Array ELISA TORCH IgG assays could reduce the turnaround time (1.5 hr vs. 5 hr by Vi- rion/Serion ELISA for 100 specimens) and were easy to use. Conclusions: BGI-Array ELISA TORCH IgG shows a good agreement with Virion/Serion ELISA methods and is suitable for clinical application.
참고문헌 (9)
- "TORCH syndrome and TORCH screening", Lancet, 335, pp.1559~1561 (1990)
- Newton ER, "Diagnosis of perinatal TORCH infections", Clin Obstet Gynecol, 42, pp.59~70 (1999)
- Stegmann BJ, "ORCH Infections. Toxoplasmosis, Other (syphilis, varicella-zoster, parvovirus B19), Rubella, Cytomegalovirus (CMV), and Herpes infections", Curr Womens Health Rep, 2, pp.253~258 (2002)
- Boutall A, "Diagnosis, etiology, and outcome of fetal ascites in a South African hospital", Int J Gynaecol Obstet, 115, pp.148~152 (2011)
- Binnicker MJ, "Multiplex detection of IgM and IgG class antibodies to Toxoplasma gondii, rubella virus, and cytomegalovirus using a novel multiplex flow immunoassay", Clin Vaccine Immunol, 17, pp.1734~1738 (2010)
- Jiang L, "Protein arrays based on biotin-streptavidin system for the simultaneous detection of TORCH infections", J Nanosci Nanotechnol, 8, pp.2286~2292 (2008)
- Owen WE, "Performance characteristics of six IMMULITE 2000 TORCH assays", Am J Clin Pathol, 126, pp.900~905 (2006)
- Jiang L, "Development of a fluorescent and colorimetric detection methods-based protein microarray for serodiagnosis of TORCH infections", Biosens Bioelectron, 24, pp.376~382 (2008)
- Mezzasoma L, "Antigen microarrays for serodiagnosis of infectious diseases", Clin Chem, 48, pp.121~130 (2002)