Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.
Abstract
Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.
참고문헌 (24)
- Montaya JG, "Toxoplasmosis", Lancet, 363, pp.1965~1976 (2004)
- Boothroyd JC, "Encyclopedia of Microbiology", pp.732~743 (2009), Elsevier
- Dubey JP, "Toxoplasma gondii infection in humans and animals in the United States", Int J Parasitol, 38, pp.1257~1278 (2008)
- Mitchell CD, "Congenital toxoplasmosis occurring in infants perinatally infected with human immunodeficiency virus 1", Pediatr Infect Dis J, 9, pp.512~518 (1990)
- McAuley J, "Early and longitudinal evaluations of treated infants and children and untreated historical patients with congenital toxoplasmosis : the Chicago collaborative treatment trial", Clin Infect Dis, 18, pp.38~72 (1994)
- Remington JS, "Infectious Diseases of the Fetus and Newborn Infant", pp.947~1091 (2006), Elsevier Saunders
- Robbins JR, "Tissue barriers of the human placenta to infection with Toxoplasma gondii", Infect Immun, 80, pp.418~428 (2012)
- Su C, "Molecular Detection of Foodborne Pathogens", pp.741~753 (2009), CBC press
- Fleiss JL, "Statistical Methods for Rates and Proportions" (2013), John Wiley and Sons
- Sterkers Y, "Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood", Diagn Microbiol Infect Dis, 71, pp.174~176 (2011)
- Dubey JP, "Topley &Wilson's Microbiology and Microbial Infections: Virology", pp.303~ (2005), Hodder Arnold
- Torgerson PR, "The global burden of congenital toxoplasmosis : a systematic review", Bull World Health Organ, 91, pp.501~508 (2013)
- Jones CD, "Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of T. gondii in aqueous humor", Invest Ophthalmol Vis Sci, 41, pp.634~644 (2000)
- Suzuki Y, "A case of congenital toxoplasmosis confirmed by detection of Toxoplasma gondii in placenta", No To Hattatsu, 30, pp.411~416 (1998)
- Homan WL, "Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR", Int J Parasitol, 30, pp.69~75 (2000)
- Filisetti D, "Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR", J Clin Microbiol, 41, pp.4826~4828 (2003)
- Amany M. Kamal, "Seropositivity of Toxoplasmosis in Pregnant Women by ELISA at Minia University Hospital, Egypt", The Korean Journal of Parasitology, 53(5), pp.605~610 (2015), 대한기생충학ㆍ열대의학회
- Da Silva MG, "Prevalence of toxoplasmosis in pregnant women and vertical transmission of Toxoplasma gondii in patients from basic units of health from Gurupi, Tocantins, Brazil, from 2012 to 2014", PLoS One, 10, pp.1~15 (2015)
- Hoveida L, "Evaluation of Toxoplasma gondii abortion cases by PCR teqnique in Isfahan, Iran", IJBPAS, 3, pp.524~531 (2014)
- Asgari Q, "Molecular genotyping of Toxoplasma gondii in human spontaneous Aborted fetuses in Shiraz, southern Iran", Iran J Public Health, 42, pp.620~625 (2013)
- Nowakowska D, "Detection of Toxoplasma gondii in human placenta by PCR and placental histologic findings", Wiad Parazytol, 48, pp.301~309 (2002)
- Sarkari B, "Evaluation of immunohistochemistry and PCR in diagnosis of toxoplasma infection in tissues of human aborted fetuses", Zahedan J Res Med Sci, 15, pp.42~ (2013)
- Abbas HH, "Detection Toxoplasma gondii by real-time PCR in abortive and pregnant women in Almuthanna province", J Inter Acad Res Mult, 2, pp.310~317 (2014)
- Bastien P, "Molecular diagnosis of toxoplasmosis", Trans R Soc Trop Med Hyg, 96(suppl), pp.205~215 (2002)