Proteomic Profiling of Serum from Patients with Tuberculosis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB. Methods: Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method. Results: Of more than 500 proteins identified, alpha-1-antitrypsin was the most discrimina- tive, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-an- titrypsin and antithrombin III increased in TB patients and showed a high variable impor- tance in the projection scores and coefficient in partial least square discriminant analysis. Conclusions: Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.
Abstract
Background: Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB. Methods: Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method. Results: Of more than 500 proteins identified, alpha-1-antitrypsin was the most discrimina- tive, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-an- titrypsin and antithrombin III increased in TB patients and showed a high variable impor- tance in the projection scores and coefficient in partial least square discriminant analysis. Conclusions: Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.
참고문헌 (27)
- Wayne LG, "Agents of newly recognized or infrequently encountered mycobacterial diseases", Clin Microbiol Rev, 5, pp.1~25 (1992)
- Neonakis IK, "Molecular diagnostic tools in mycobacteriology", J Microbiol Methods, 75, pp.1~11 (2008)
- Zhang Z, "The road from discovery to clinical diagnostics: lessons learned from the first FDA-cleared in vitro diagnostic multivariate index assay of proteomic biomarkers", Cancer Epidemiol Biomarkers Prev, 19, pp.2995~2999 (2010)
- Emily S. Boja, "The Path to Clinical Proteomics Research: Integration of Proteomics, Genomics, Clinical Laboratory and Regulatory Science", Annals of Laboratory Medicine, 31(2), pp.61~71 (2011), 대한진단검사의학회
- Cheng FY, "Absolute protein quantification by LC", MS(E) for global analysis of salicylic acid-induced plant protein secretion responses, 2009, pp.8, 82~93
- Wallis RS, "Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice", Lancet, 375, pp.1920~1937 (2010)
- Agranoff D, "Identification of diagnostic markers for tuberculosis by proteomic fingerprinting of serum", Lancet, 368, pp.1012~1021 (2006)
- Wong CT, "Changes in serum proteins (albumin, immunoglobulins and acute phase proteins) in pulmonary tuberculosis during therapy", Tubercle, 71, pp.193~197 (1990)
- Emmett M, "Protein abnormalities in adult respiratory distress syndrome, tuberculosis, and cystic fibrosis sera", Proc Soc Exp Biol Med, 184, pp.74~82 (1987)
- Grange JM, "A study of acute-phase reactant proteins in Indonesian patients with pulmonary tuberculosis", Tubercle, 65, pp.23~39 (1984)
- Schaller J, "Human blood plasma proteins : structure and function" (2008), John Wiley & Sons
- Laurell CB, "The electrophoretic α1-globulin pattern of serum in α1-antitrypsin deficiency", COPD, 10(1), pp.3~8 (2013)
- Lisowska-Myjak B, "AAT as a diagnostic tool", Clin Chim Acta, 352, pp.1~13 (2005)
- Miravitlles M, "Alpha-1-antitrypsin and other proteinase inhibitors", Curr Opin Pharmacol, 12, pp.309~314 (2012)
- Cylwik B, "Relationship between serum acute-phase proteins and high disease activity in patients with rheumatoid arthritis", Adv Med Sci, 55, pp.80~85 (2010)
- Wu W, "GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis", Proteomics Clin Appl, 6, pp.152~162 (2012)
- Morgan BP, "Regulation of the complement membrane attack pathway", Crit Rev Immunol, 19, pp.173~198 (1999)
- Sai Baba KS, "Complement activation in pulmonary tuberculosis", Tubercle, 71, pp.103~107 (1990)
- Turken O, "Hemostatic changes in active pulmonary tuberculosis", Int J Tuberc Lung Dis, 6, pp.927~932 (2002)
- Robson SC, "Acute-phase response and the hypercoagulable state in pulmonary tuberculosis", Br J Haematol, 93, pp.943~949 (1996)
- Kozakiewicz L, "The role of B cells and humoral immunity in Mycobacterium tuberculosis infection", Adv Exp Med Biol, 783, pp.225~250 (2013)
- Kirwan GM, "Building multivariate systems biology models", Anal Chem, 84, pp.7064~7071 (2012)
- Fan L, "Identification of metabolic biomarkers to diagnose epithelial ovarian cancer using a UPLC", QTOF, Acta Oncol, pp.2012, 51[DOI]
- Liu Y, "A metabolic profiling analysis of symptomatic gout in human serum and urine using high performance liquid chromatography-diode array detector technique", Clin Chim Acta, 412, pp.2132~2140 (2011)
- Wang X, "Urine metabolomics analysis for biomarker discovery and detection of jaundice syndrome in patients with liver disease", Mol Cell Proteomics, 11, pp.370~380 (2012)
- Li Y, "Label-free quantitative proteomic analysis reveals dysfunction of complement pathway in peripheral blood of schizophrenia patients: evidence for the immune hypothesis of schizophrenia", Mol Biosyst, 8, pp.2664~2671 (2012)
- Ray S, "Proteomic investigation of falciparum and vivax malaria for identification of surrogate protein markers", PLoS One, 7, pp.41751~ (2012)