마늘껍질 70% 에탄올 추출물의 인간 암세포 증식억제 활성
Antiproliferation effects of ethanol extract of garlic peels on human cancer cell lines
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Ethanol extract of garlic peels (GPE) was investigated for its antiproliferative effects on human cancer cell lines. Human lung cancer cell line A549 treated with 500 μg/mL GPE resulted in the growth inhibition of A549 by 90%. In stomach cancer cell AGS proliferation inhibition activity, GPE showed 45% and 71% inhibition of AGS growth at 1,000 μg/mL and 2,000 μg/mL, respectively. GPE inhibited the growth of the breast cancer cells MCF-7 effectively at low concentration and showed 78% and 90% inhibitions of MCF-7 growth at 200 μg/mL and 500 μg/mL , respectively. GPE showed very significant antiproliferation effect on liver cancer cell line Hep3B and inhibited Hep3B cell growth by 57% at 100 μg/mL, and the inhibition’s rate increased up to 87% at 500 μg/mL. Antiproliferation effect of GPE on colorectal cancer cell HT-29 showed 15% reduction of HT-29 cell growth at 200 μg/mL and the growth rate was reduced in a dose dependent manner up to 1,000 μg/mL. These results indicated that GPE had high antiproliferation effects on breast and liver cancer cell lines at low concentrations (200 μg/mL), and by higher concentrations over 500 μg/mL, GPE inhibited the growth of A549 and HT-29. The results of our study suggested the potential use of garlic peels for use as an excellent antiproliferative substance for human cancer cells.
Abstract
Ethanol extract of garlic peels (GPE) was investigated for its antiproliferative effects on human cancer cell lines. Human lung cancer cell line A549 treated with 500 μg/mL GPE resulted in the growth inhibition of A549 by 90%. In stomach cancer cell AGS proliferation inhibition activity, GPE showed 45% and 71% inhibition of AGS growth at 1,000 μg/mL and 2,000 μg/mL, respectively. GPE inhibited the growth of the breast cancer cells MCF-7 effectively at low concentration and showed 78% and 90% inhibitions of MCF-7 growth at 200 μg/mL and 500 μg/mL , respectively. GPE showed very significant antiproliferation effect on liver cancer cell line Hep3B and inhibited Hep3B cell growth by 57% at 100 μg/mL, and the inhibition’s rate increased up to 87% at 500 μg/mL. Antiproliferation effect of GPE on colorectal cancer cell HT-29 showed 15% reduction of HT-29 cell growth at 200 μg/mL and the growth rate was reduced in a dose dependent manner up to 1,000 μg/mL. These results indicated that GPE had high antiproliferation effects on breast and liver cancer cell lines at low concentrations (200 μg/mL), and by higher concentrations over 500 μg/mL, GPE inhibited the growth of A549 and HT-29. The results of our study suggested the potential use of garlic peels for use as an excellent antiproliferative substance for human cancer cells.
참고문헌 (21)
- 이형범, "송이버섯과 동충하초 균사체를 혼합 배양한 한방추출물의 발효에 의한 생리활성", 대한본초학회지, 23(1), pp.1~8 (2008), 대한본초학회
- 민경진, "Yacon 추출물의 항산화 및 항암 활성", 한국식품위생안전성학회지, 23(2), pp.163~168 (2008), 한국식품위생안전성학회
- Nam SH, "Isolation of cytotoxic substances from Chrysanthemum boreale M", J Korean Soc Appl Biol Chem, 38, pp.273~277 (1995)
- Wu CC, "Effects of organosulfur compounds from garlic oil on the antioxidation system in rat liver and red blood cells", Food Chem Toxicol, 39, pp.563~569 (2001)
- 이영춘, "Protopectinase를 이용하여 제조한 마늘 단세포화물의품질특성", 한국식품저장유통학회지, 13(3), pp.351~356 (2006), 한국식품저장유통학회
- Lim SC, "Structures of flavonoids and furostanol glycosides isolated from the bulbs of Allium victorialis L", J Kor Soc Hort Sci, 37, pp.675~679 (1996)
- Nuutila AM, "Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity", Food Chem, 81, pp.485~493 (2003)
- 조현주, "고콜레스테롤 식이 섭취시 1% 마늘가루 첨가가 혈액 및 간조직 중 지질과 혈중 유리 아미노산 농도에 미치는 영향", 한국식품영양과학회지, 31(1), pp.98~103 (2002), 한국식품영양과학회
- Steiner M, "Aged garlic extract, a modulator of cardiovascular risk factors: a dose-finding study on the effects of AGE on platelet functions", J Nutr, 131, pp.980S~984S (2001)
- Kim HK, "Physiological activity and antioxidative effect of garlic (Allium sativum L.) extract", Food Sci Biotechnol, 11, pp.500~506 (2002)
- 김라정, "발효 마늘껍질의 이화학적 특성 및 항산화 활성", 한국식품영양과학회지, 39(12), pp.1731~1738 (2010), 한국식품영양과학회
- Young FM, "Modification of MTT assay conditions to examine the cytotoxic effects of amitraz on the human lymphoblastoid cell line, WIL2NS", Toxocol In Vitro, 19, pp.1051~1059 (2005)
- 김만철, "버섯 균사체 혼합 배양 추출물의 항균, 항산화 및 항암 활성", KSBB Journal, 23(2), pp.158~163 (2008), 한국생물공학회
- 하지혜, "홍경천 뿌리 및 캘러스 초음파 추출물의 항암활성 비교", 한국식품과학회지, 41(5), pp.552~559 (2009), 한국식품과학회
- 김영, "저온고압 추출공정을 이용한 매자나무 수피의 항산화 및 항암활성 증진", 한국식품과학회지, 41(3), pp.284~291 (2009), 한국식품과학회
- 박은미, "여지초의 in vitro 항암효과 및 면역세포 활성화에 미치는 영향", 한국식품영양과학회지, 36(4), pp.377~382 (2007), 한국식품영양과학회
- 김예진, "Hot Water Leaves Extracts of Zizyphus jujube Exert Antioxidative Effects in Vitro and Cytotoxicity in Human Cancer Cell Lines", Horticulture, Environment, and Biotechnology, 52(6), pp.635~640 (2011), 한국원예학회
- 임선영, "콩 종류별 항돌연변이 및 암세포 증식 억제 효과 비교", 생명과학회지, 20(10), pp.1532~1537 (2010), 한국생명과학회
- Challier B, "Garlic, onion and cereal fibre as protective factors for breast cancer: a French case-control study", Eur J Epidemiol, 14, pp.737~747 (1998)
- 함승시, "산마늘 추출물의 항돌연변이원성 및 세포독성 효과", 한국식품저장유통학회지, 11(2), pp.221~226 (2004), 한국식품저장유통학회
- 권석형, "버섯 균사체에 의한 암세포 성장억제 효과", 한국식품영양학회지, 16(1), pp.15~21 (2003), 한국식품영양학회