Composition Information of Allium Tuberosum Juice Extract and Its Anticancer Effect in C6 GLIOMA CELLS
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Cancer is one of the leading causes of death worldwide. Cancer motility plays a central role in malignant tumorigenesis. Hence, several studies have been focused on investigating effective treatments for this disease. Natural compounds are among the potential therapeutic agents against cancer. Allium tuberosum has been used in Korean traditional medicine to improve stamina, and has been recently reported to possess anti-cancer properties. In the present study, we aimed to investigate the effect of A. tuberosum juice extracts(ATS) on migration and proliferation of glioma cells. The composition of ATS was determined using gas chromatography-mass spectrometry(GC/MS). Cell viability and proliferation were determined using MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and cell migration was assessed using scratch wound-healing assay. The expression levels of proteins were determined using western blot with specific antibodies such as the extra cellular signal-regulated kinase 1/2(ERK1/2) and MMP2. Our data showed that ATS did not affect cell viability at a concentration of 300 µg/mL in C6 glioma cell lines. However, ATS significantly reduced the migration of C6 glioma cells. Moreover, ATS significantly suppressed ERK1/2 phosphorylation and MMP2 expression at dosages of 100 and 300 µg/mL. A total of 34 constituents of ATS were detected using GG/MS. Therefore, we suggest that ATS is a potential agent against glioma.
Abstract
Cancer is one of the leading causes of death worldwide. Cancer motility plays a central role in malignant tumorigenesis. Hence, several studies have been focused on investigating effective treatments for this disease. Natural compounds are among the potential therapeutic agents against cancer. Allium tuberosum has been used in Korean traditional medicine to improve stamina, and has been recently reported to possess anti-cancer properties. In the present study, we aimed to investigate the effect of A. tuberosum juice extracts(ATS) on migration and proliferation of glioma cells. The composition of ATS was determined using gas chromatography-mass spectrometry(GC/MS). Cell viability and proliferation were determined using MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and cell migration was assessed using scratch wound-healing assay. The expression levels of proteins were determined using western blot with specific antibodies such as the extra cellular signal-regulated kinase 1/2(ERK1/2) and MMP2. Our data showed that ATS did not affect cell viability at a concentration of 300 µg/mL in C6 glioma cell lines. However, ATS significantly reduced the migration of C6 glioma cells. Moreover, ATS significantly suppressed ERK1/2 phosphorylation and MMP2 expression at dosages of 100 and 300 µg/mL. A total of 34 constituents of ATS were detected using GG/MS. Therefore, we suggest that ATS is a potential agent against glioma.