Clinical and Microbiological Characteristics of Six Staphylococcus pettenkoferi Isolates From Blood Samples
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood specimens, including one pathogen and 5 contaminants and catheter colonizers. Brucker Biotyper (Brucker Daltonics, Bremen, Germany) and molecular typing with 16S rRNA gene sequencing confirmed the 6 isolates as S. pettenkoferi. The conventional phenotypic identification of these isolates is not reliable owing to their inconsistent biochemical characteristics. Five of the 6 isolates were found to be resistant to oxacillin, and all isolates showed susceptibility to vancomycin and linezolid. For accurate identification of this novel species, advanced methods by using Brucker Biotyper or molecular methods such as 16S rRNA gene sequencing are required.
Abstract
Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood specimens, including one pathogen and 5 contaminants and catheter colonizers. Brucker Biotyper (Brucker Daltonics, Bremen, Germany) and molecular typing with 16S rRNA gene sequencing confirmed the 6 isolates as S. pettenkoferi. The conventional phenotypic identification of these isolates is not reliable owing to their inconsistent biochemical characteristics. Five of the 6 isolates were found to be resistant to oxacillin, and all isolates showed susceptibility to vancomycin and linezolid. For accurate identification of this novel species, advanced methods by using Brucker Biotyper or molecular methods such as 16S rRNA gene sequencing are required.
참고문헌 (15)
- Trülzsch K, "“Staphylococcus pettenkoferi,” a novel staphylococcal species isolated from clinical specimens", Diagn Microbiol Infect Dis, 43, pp.175~182 (2002)
- Trülzsch K, "Staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens", Int J Syst Evol Microbiol, 57, pp.1543~1548 (2007)
- Loïez C, "First case of osteomyelitis caused by “Staphylococcus pettenkoferi.”", J Clin Microbiol, 45, pp.1069~1071 (2007)
- Song SH, "Human bloodstream infection caused by Staphylococcus pettenkoferi", J Med Microbiol, 58, pp.270~272 (2009)
- d’Azevedo PA, "Characterization of a new coagulase-negative Staphylococcus species (Staphylococcus pettenkoferi) isolated from blood cultures from a hospitalized patient in Porto Alegre, Brazil", Rev Soc Bras Med Trop, 43, pp.331~332 (2010)
- Garza-González E, "Diversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance", J Med Microbiol, 59, pp.323~329 (2010)
- Mihaila L, "A dual outbreak of bloodstream infections with linezolid-resistant Staphylococcus epidermidis and Staphylococcus pettenkoferi in a liver Intensive Care Unit", Int J Antimicrob Agents, 40, pp.472~474 (2012)
- Moon HW, "Performance of the Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories.", J Med Microbiol, 62, pp.1301~1306 (2013)
- Schuurman T, "Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting", J Clin Microbiol, 42, pp.734~740 (2004)
- Clinical and Laboratory Standards Institute, "Interpretive criteria for identification of bacteria and fungi by DNA target sequencing: approved guideline, MM18-A", Clinical and Laboratory Standards Institute, ~ (2008)
- Clarridg JE 3rd, "Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases", Clin Microbiol Rev, 17, pp.840~862 (2004)
- Woo PC, "Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories", Clin Microbiol Infect, 14, pp.908~934 (2008)
- Drozenová J, "Characteristics of coagulase-negative staphylococci isolated from hemocultures", Epidemiol Mikrobiol Imunol, 49, pp.51~58 (2000)
- Koksal F, "Antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in Turkey", Microbiol Res, 164, pp.404~410 (2009)
- Carbon C, "MRSA and MRSE: is there an answer?", Clin Microbiol Infect, 6, pp.S17~22 (2000)