Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests
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Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.
Abstract
Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.
참고문헌 (16)
- de Kraker ME, "Clinical impact of antimicrobial resistance in European hospitals: excess mortality and length of hospital stay related to methicillin-resistant Staphylococcus aureus bloodstream infections", Antimicrob Agents Chemother, 55, pp.1598~1605 (2011)
- Kim SH, "Outcome of vancomycin treatment in patients with methicillin-susceptible Staphylococcus aureus bacteremia", Antimicrob Agents Chemother, 52, pp.192~197 (2008)
- Bates DW, "Contaminant blood cultures and resource utilization. The true consequences of false-positive results", JAMA, 265, pp.365~369 (1991)
- Romero-Gómez MP, "Evaluation of the BinaxNOW PBP2a assay for the direct detection of methicillin resistance in Staphylococcus aureus from positive blood culture bottles", Diagn Microbiol Infect Dis, 72, pp.282~284 (2012)
- Carretto E, "Comparison of the Staphylococcus QuickFISH BC test with the tube coagulase test performed on positive blood cultures for evaluation and application in a clinical routine setting", J Clin Microbiol, 51, pp.131~135 (2013)
- Gröbner S, "Evaluation of the BD GeneOhm StaphSR assay for detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from spiked positive blood culture bottles", J Clin Microbiol, 47, pp.1689~1694 (2009)
- Lagacé-Wiens PR, "Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time.", J Clin Microbiol, 50, pp.3324~3328 (2012)
- Dhiman N, "Evaluation of the BinaxNOW Staphylococcus aureus test for rapid identification of Gram-positive cocci from VersaTREK blood culture bottles", J Clin Microbiol, 51, pp.2939~2942 (2013)
- Yossepowitch O, "A cost-saving algorithm for rapid diagnosis of Staphylococcus aureus and susceptibility to oxacillin directly from positive blood culture bottles by combined testing with BinaxNOW® S. aureus and Xpert MRSA", SA Assay, 2014, pp.78, 352~355
- Spencer DH, "Validation and implementation of the GeneXpert MRSA", SA blood culture assay in a pediatric setting, 2011, pp.136, 690~694
- Qian Q, "Rapid identification of Staphylococcus aureus directly from Bactec blood culture broth by the BinaxNOW S. aureus test", J Clin Microbiol, 52, pp.319~320 (2014)
- Corso A, "Improvement of a latex agglutination test for the evaluation of oxacillin resistance in coagulase-negative staphylococci", Diagn Microbiol Infect Dis, 50, pp.223~225 (2004)
- Laurent F, "MRSA harboring mecA variant gene mecC, France", Emerg Infect Dis, 18, pp.1465~1467 (2012)
- Eigner U, "Evaluation of a rapid direct assay for identification of bacteria and the mecA and van genes from positive-testing blood cultures", J Clin Microbiol, 43, pp.5256~5262 (2005)
- Szabados F, "Identical MALDI TOF MS-derived peak profiles in a pair of isogenic SCCmec-harboring and SCCmec-lacking strains of Staphylococcus aureus", J Infect, 65, pp.400~405 (2012)