NF-kappaB 경로의 억제에 의한 lipopolysaccharide로 활성화된 대식세포의 토종다래(Actinidia arguta) 추출물의 항염증 효과
Anti-inflammation effect of hardy kiwifruit (Actinidia arguta) extract in lipopolysaccharide-stimulated macrophages by suppressing NF-kappaB pathway
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The aim of this study was to investigate the antioxidant and intracellular anti-inflammatory efficacy of hardy kiwifruit extracted with hot water (HKW), 70% ethanol (HKE), and 70% acetone (HKA) in RAW 264.7 macrophages. In order to evaluate the anti-inflammatory effect of hardy kiwifruit extracts, RAW 264.7 macrophages were stimulated with lipopolysaccharide (LPS) to induce the production of inflammation-related factors, which were measured by Western blotting and real-time PCR methods. i-NOS, COX-2, NF-κB protein, and mRNA expression showed a concentration-dependent anti-inflammatory effect. The decreases in the mRNA expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were concentration-dependent in HKE. Further, the antioxidant effects of hardy kiwifruit on the total polyphenol contents, electron donating ability and ABTS+ radical scavenging activity were evaluated. The total polyphenol contents of HKW, HKE, and HKA were 15.54±0.29, 14.41±0.26, and 16.82±0.27 mg TAE/g, respectively, whereas the electron donating abilities at 1,000 μg/mL of HKW, HKE, and HKA were 30.8, 36.1, and 37.4%, respectively. The ABTS+ radical scavenging activity was fond to be concentration dependent. The nitric oxide (NO) production inhibition activities at 2,000 μg/mL of HKE was 42.8%. In conclusion, anti-oxidant and anti-inflammatory test results indicate that hardy kiwifruit can be used as potential anti-inflammatory agents via NF-κB inhibition.
Abstract
The aim of this study was to investigate the antioxidant and intracellular anti-inflammatory efficacy of hardy kiwifruit extracted with hot water (HKW), 70% ethanol (HKE), and 70% acetone (HKA) in RAW 264.7 macrophages. In order to evaluate the anti-inflammatory effect of hardy kiwifruit extracts, RAW 264.7 macrophages were stimulated with lipopolysaccharide (LPS) to induce the production of inflammation-related factors, which were measured by Western blotting and real-time PCR methods. i-NOS, COX-2, NF-κB protein, and mRNA expression showed a concentration-dependent anti-inflammatory effect. The decreases in the mRNA expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were concentration-dependent in HKE. Further, the antioxidant effects of hardy kiwifruit on the total polyphenol contents, electron donating ability and ABTS+ radical scavenging activity were evaluated. The total polyphenol contents of HKW, HKE, and HKA were 15.54±0.29, 14.41±0.26, and 16.82±0.27 mg TAE/g, respectively, whereas the electron donating abilities at 1,000 μg/mL of HKW, HKE, and HKA were 30.8, 36.1, and 37.4%, respectively. The ABTS+ radical scavenging activity was fond to be concentration dependent. The nitric oxide (NO) production inhibition activities at 2,000 μg/mL of HKE was 42.8%. In conclusion, anti-oxidant and anti-inflammatory test results indicate that hardy kiwifruit can be used as potential anti-inflammatory agents via NF-κB inhibition.
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