한국 및 중국 재배종 미숙과 복분자의 엘라그산 함량의 비교
Comparison of ellagic acid contents in Korean and Chinese cultivated species of unripe black raspberries
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
The goal of this study was to investigate the extracts of ellagic acid in Korean and Chinese black raspberries according to harvest time and extraction solvent. This study was also performed to establish a simple and reliable high-performance liquid chromatography-ultraviolet (HPLC/UV) analytical method for the determination of ellagic acid for quality control and to establish baseline data of extracts of Rubus occidentalis (uRo) and Rubus chingii (uRc) fruits as healthy functional food ingredients. uRo and uRc were each extracted with 0%, 5%, 30%, and 70% ethanol. Among the extracts of these two varieties, the 5% ethanol extracts of uRo and uRc showed the highest contents of ellagic acid, 16.56 and 17.27 mg/g, respectively. The specificity was satisfied with retention time and photo-diode array (PDA) spectrum by analysis of ellagic acid using HPLC and comparison with a standard compound. Ellagic acid was validated for its limit of detection (LOD), limit of quantitation (LOQ), precision, and accuracy. It showed a high linearity in the calibration curve, with a coefficient of determination (R2) of 0.9999, and the LOD and LOQ values were 0.25 and 0.77 μg/mL, respectively. The results showed that R. occidentalis and R. chingii can be used simultaneously as healthy functional ingredients and natural medicines.
Abstract
The goal of this study was to investigate the extracts of ellagic acid in Korean and Chinese black raspberries according to harvest time and extraction solvent. This study was also performed to establish a simple and reliable high-performance liquid chromatography-ultraviolet (HPLC/UV) analytical method for the determination of ellagic acid for quality control and to establish baseline data of extracts of Rubus occidentalis (uRo) and Rubus chingii (uRc) fruits as healthy functional food ingredients. uRo and uRc were each extracted with 0%, 5%, 30%, and 70% ethanol. Among the extracts of these two varieties, the 5% ethanol extracts of uRo and uRc showed the highest contents of ellagic acid, 16.56 and 17.27 mg/g, respectively. The specificity was satisfied with retention time and photo-diode array (PDA) spectrum by analysis of ellagic acid using HPLC and comparison with a standard compound. Ellagic acid was validated for its limit of detection (LOD), limit of quantitation (LOQ), precision, and accuracy. It showed a high linearity in the calibration curve, with a coefficient of determination (R2) of 0.9999, and the LOD and LOQ values were 0.25 and 0.77 μg/mL, respectively. The results showed that R. occidentalis and R. chingii can be used simultaneously as healthy functional ingredients and natural medicines.
참고문헌 (15)
- Bae GH, "The medicinal plant of Korea", pp.231~ (2000), Kyohasa Publishing Co Ltd
- 유기석, "Internal Transcribed Spacer (ITS) Regions Reveals Phylogenic Relationships of Rubus Species Cultivated in Korea", 한국약용작물학회지, 17(3), pp.165~172 (2009), 한국약용작물학회
- 유기석, "Phylogenic Relationship of Rubus Cultivated in Korea Revealed by Chloroplast DNA Spacers", 한국약용작물학회지, 18(4), pp.266~272 (2010), 한국약용작물학회
- 이소미, "광양 재래종 복분자의 항산화 활성", 한국식품영양과학회지, 41(3), pp.327~332 (2012), 한국식품영양과학회
- KFRI, "Rubus coreanus Miq", Research Report, 484, pp.1~193 (2012)
- 김이선, "토종 복분자 Rubus coreanus와 외래종 복분자 Rubus occidentalis 추출물의 항산화능 비교", 한국식품영양과학회지, 43(9), pp.1357~1362 (2014), 한국식품영양과학회
- 최혜란, "복분자 미숙과 물추출물의 콜레스테롤 개선 효과", 한국식품영양과학회지, 42(12), pp.1899~1907 (2013), 한국식품영양과학회
- 채규서, "복분자 추출물의 기능성 원료 표준화를 위한 지표성분 Ellagic acid 분석법", 산업식품공학, 18(4), pp.355~358 (2014), 한국산업식품공학회[DOI]
- Jung MA, "Anti-obesity effects on unripe Rubus coreanus Miquel extract in high fat diet-induced obese mice", Int J Biochem Res & Rev, 5, pp.20~26 (2015)[DOI]
- Oh DR, "Antiobesity effects of unripe Rubus coreanus Miquel and its constituents: An in vitro and in vivo characterization of the underlying mechanism", Evid Based Complement Alternat Med, 2016, pp.1~15 (2016)
- 정명아, "미숙과 복분자 열매 추출물이 고콜레스테롤 식이를 섭취시킨 C57BL", 6 Mice의 지질대사 개선 효과, 2014, pp.43(5), 650~655[DOI]
- 김윤정, "고창 복분자의 기능성원료 표준화를 위한 지표성분으로서 Ellagic Acid의 분석법 개발", 한국식품영양과학회지, 41(11), pp.1554~1558 (2012), 한국식품영양과학회
- "Guideline for the validation of drug analysis procedure"[URL]
- "Guideline for the validation of drug analysis procedure"[URL]
- 이헌호, "재배종 및 야생 산딸기의 기능성 성분 함량과 항산화 활성", 원예과학기술지, 32(1), pp.115~122 (2014), 한국원예학회[DOI]