Amylase와 glucose isomerase를 이용한 starch로부터 D-fructose의 생산
Production of D-fructose from starch by amylase and glucose isomerase
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Two commercial enzymes, amylase and glucose isomerase, were applied in sequential and simultaneous enzyme reactions to produce D-fructose from starch. 80 U/g Fungamyl 800L, 35 U/mL glucose isomerase, and 20% of starch were the most effective concentrations for this reaction. In the sequential enzyme reaction, Fungamyl 800L maximally produced 56 g/L D-glucose from starch at pH 5.0 and 55℃ for 2 h. Consequently, glucose isomerase was reacted with D-glucose produced from starch by Fungamyl 800L at pH 6.0 and 55℃ for 2 h. This resulted in the production of 18.5 g/L D-fructose, however, the production of D-fructose did not increase any more after a 2 h enzyme reaction. In the simultaneous enzyme reaction, Fungamyl 800L and glucose isomerase were simultaneously reacted with starch at pH 5.0 and 55℃. In the simultaneous reaction, D-fructose production continued to increase as the reaction time increased and finally 39 g/L D-fructose was produced from starch after 18 h. Based on these results, the simultaneous enzyme reaction was found to be more efficient than the sequential enzyme reaction with respect to the production of D-fructose from starch using amylase and glucose isomerase.
Abstract
Two commercial enzymes, amylase and glucose isomerase, were applied in sequential and simultaneous enzyme reactions to produce D-fructose from starch. 80 U/g Fungamyl 800L, 35 U/mL glucose isomerase, and 20% of starch were the most effective concentrations for this reaction. In the sequential enzyme reaction, Fungamyl 800L maximally produced 56 g/L D-glucose from starch at pH 5.0 and 55℃ for 2 h. Consequently, glucose isomerase was reacted with D-glucose produced from starch by Fungamyl 800L at pH 6.0 and 55℃ for 2 h. This resulted in the production of 18.5 g/L D-fructose, however, the production of D-fructose did not increase any more after a 2 h enzyme reaction. In the simultaneous enzyme reaction, Fungamyl 800L and glucose isomerase were simultaneously reacted with starch at pH 5.0 and 55℃. In the simultaneous reaction, D-fructose production continued to increase as the reaction time increased and finally 39 g/L D-fructose was produced from starch after 18 h. Based on these results, the simultaneous enzyme reaction was found to be more efficient than the sequential enzyme reaction with respect to the production of D-fructose from starch using amylase and glucose isomerase.
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