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양배추의 항산화성 추출물 제조를 위한 마이크로웨브 추출조건 설정

Pre-establishment of Microwave-Assisted Extraction Conditions for Antioxidative Extracts from Cabbage

김현구|최유경|권중호|노정은
한국식품저장유통학회 (The Korean Society of Food Preservation)|2005.02|Food Science and Preservation|제12권 제1호|pp. 62-67

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참고문헌

키워드

yieldtotal phenolcabbageelectron donating abilitymicrowave extraction
주제분류식품과학

초록

양배추의 고부가가치 이용방안 연구의 일환으로 선택적 가열 및 추출특성이 알려진 마이크로파 에너지(50 W, 2,450 MHz)를 이용하여 양배추 추출물의 총 수율과 총 페놀 함량 및 전자공여능의 효율적 추출조건을 설정하였다. 에탄올(50%) 용매에서 건조 양배추의 입자 크기는 20 mesh 정도가 적당하였고, 시료와 용매비는 생체시료(수분 90.2%)와 건조시료(수분 4.5%) 모두에서 1:10(5 g/50 mL) 정도가 가장 타당한 것으로 나타났다. 추출용매의 영향에서는 생체시료에서는 50% 에탄올>100% 에탄올>물의 순으로, 건조시료에서는 50% 에탄올>물>100% 에탄올의 순으로 높은 추출수율을 보여주었다. 추출물의 총 페놀 함량과 전자공여능은 추출 3분 이후에는 거의 차이가 없는 경향을 보여주었고, 생양배추 시료에서는 100% 에탄올>50% 에탄올>물의 순으로, 건조 양배추 시료에서는 50% 에탄올>물> 100% 에탄올 순으로 높은 함량을 보여주었다.

Abstract

Microwave-assisted extraction (50 W, 2,450 MHz, MAE) with properties of selective heating and subsequent extraction for certain phytochemicals from natural materials was applied to pre-establish the extraction conditions for total yield, total phenolics, and electron donating ability (EDA) from Brossica oleacea. The experiments with 50% EtOH solvent showed that 20 mesh in particle size of cabbage flake (moisture 4.5%) and 1:10 (g/mL) in the sample to solvent ratio for both raw (moisture 90.2%) and flake cabbages were optimal for MAE efficiency. Under these conditions, total yield increased with extraction time, which was highest for raw cabbage extracts in 50% EtOH solvent, followed by 100% EtOH and water. While that of flake cabbage extracts was highest in 50% EtOH, followed by water and 100% EtOH. The contents of total phenolics and EDA in extracts gradually increased after 3 min of MAE, which were highest when using 100% EtOH solvent, followed by 50% EtOH and water in raw cabbage and 50% EtOH, followed by water and 100% EtOH in flake cabbage, respectively.

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