성숙에 따른 뜰보리수(Elaeagnus multiflora THUNB.) 과실 에탄올 추출물의 항산화 효과
Anti-oxidant Activity of Ethanol Extracts from Fruits of Elaeagnus multiflora THUNB. during Maturation
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참고문헌
초록
본 연구는 뜰보리수 열매의 식품영양학적 평가 및 기능성 물질 발굴의 일환으로 뜰보리수 열매를 이용하여 성숙 과정 중의 free radical 소거능을 비롯한 SOD유사활성, 아질산염 소거작용, xanthine oxidase 저해활성 등의 항산화 활성을 조사하였다. 뜰보리수 에탄올 추출물의 전자공여능은 성숙함에 따라 감소하는 경향을 나타내었으며, 에탄올 추출물의 농도가 높을수록 전자공여능의 효과는 더욱 높게 나타났다. 에탄올 추출물의 SOD 유사활성은 성숙정도에 따른 증가하였고, 농도에 비레하였다. 뜰보리수 열매의 에탄올 추출물의 아질산염 소거능은 pH가 낮을수록 높았으며, 농도가 증가할수록 아질산염 소거 작용이 높게 나타났다. Xanthine oxidase 활성 저해능은 완숙과 과숙과실의 추출물에 비해서 미숙과실 추출물에서 높았다.감사의 글
Abstract
This study was designed to analyze the electron donating ability(EDA), superoxide dismutase(SOD)-like activity, nitrite scavenging ability and xanthine oxidase for food nutritional evaluation and excavation of functional materials in fruits of Elaeagnus multiflora THUNB. The electron donating ability of ethanol extracts in the 1.0 mg/ml concentration of extracts was higher in extracts of unripe fruits than others. The superoxide dismutase (SOD)-like activity was increased according to increasing of the extracts concentration. The SOD-like activity was highest in the extract of unripe fruits among the extracts. The nitrite scavenging ability of unripe fruits ethanol extracts in 1.0 mg/ml of extraction solution in pH 1.2 was higher than extract of ripe and Overripe fruits. The nitrite scavenging ability was decreased according to increasing of pH. Also, inhibition of xanthine oxidase activity were higher in extracts of unripe fruits than those in extracts of ripe and over-ripe fruits.
참고문헌 (33)
- Lee, H.S, "Dietary fiber intake of Korea", J. Korean Soc. Food Sci. Nutr, 25, pp.540~548 (1997)
- Park, S.Y., "Screening and isolation of the antitumor agents from medicinal plants(Ⅰ)", Korean J. Pharmacogn, 23, pp.264~267 (1992)
- Shim, J.H, "Screening of antioxidant in fructus mune(Prunus mune Sieb.et Zucc.)Extract", J. Korean Soc. Agric. Chem. Biotechnol, 45, pp.119~123 (2002)
- Ames. B.N, "Oxidants, antioxidants, and the degenerative diease of aging", Proc. Natl. Acad. Sci, 90, pp.7915~7922 (1993)
- Cho, J.Y, "Isolation and identification of 3-methoxy-4-hydroxybenzoic acid and 3-methoxy-4-hydroxy-cinamic acid with antioxidative and antimicrobial activity from hot water extracts of Hovenia dulcis Thunb and conformation of their antioxidative and antimicrobial activity", Korean J. Food Sci. Technol, 32, pp.1403~1408 (2000)
- Wee, J.H, "Isolation of 4-hydroxycinnamic acid, 3-methoxy-4-hydroxycinnamic acid and 3,4-dihydroxybenzoic acid with antioxidative and antimicrobial activity from peanut(Arachis hypogaea)shell", Food Sci. Biotechnol, 10, pp.551~556 (2001)
- 유미희, "생대추(풋대추) 과육 및 씨 추출물의 항산화 효과 및 항산화 성분", 한국식품과학회지, 38(1), pp.128~134 (2006), 한국식품과학회
- Moon, J.H, "Functional components and physiological activity of tea", J. Korean Tea Soc, 1, pp.175~179 (1995)
- Ramarathnam, N, "The contribution of plant food antioxidants to human health", Trends Food Sci, 6, pp.75~82 (1995)
- Jang, M.J, "Effects of Antioxidative, DPPH Radical Scavenging Activity and Antithrombogenic by the Extract of Sancho (Zanthoxylum Schinifolium)", Korean Nutrition Society, 38, pp.386~394 (2005)
- Oh, H.M, "Effects of dried leaf powders, water and ethanol extracts of persimmon and green tea leaves on lipid metabolism and antioxidative capacity in 12-month-old rats", Korean Nutrition Society, 34, pp.285~298 (2001)
- Yu, M.H, "Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis rehder", Korean J. Food Sci. Technol, 38, pp.1281~ (2006)
- 손종연, "석류씨 추출물의 항산화 및 항균활성", 한국식품조리과학회지, 21(2), pp.171~179 (2005), 한국식품조리과학회
- 조무생, "원색한국수목도감", pp.372~ (1989), 아카데미, 서울
- 이우철, "한국식품명고", pp.715~ (1996), 아카데미서적. 서울
- 고경식, "식물도감 한국의 야생식물", pp.454~455 (2003), 일진사. 서울
- 박종희, "한국약초도감", pp.953~954 (2004), 도서출판 신일상사. 서울
- Blosis, M.L., "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1224 (1958)
- Marklund, S, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur. J. Biochem, 47, pp.469~474 (1974)
- Kato, H, "Inhibitory of nitrosamine formation by nondilyzable melanoidins", Agric. Biol. Chem, 51, pp.1333~1338 (1987)
- Stirpe, F, "The regulation of rat liver xanthine oxidase", J. Biol. Chem, 244, pp.3855~3861 (1969)
- 김수민, "솔잎 및 녹차추출물의 항산화성 및 아질산염 소거작용", 한국축산식품학회지, 22(1), pp.13~19 (2002), 한국축산식품학회
- Cha, W.S, "Antioxidant activity of phenol conpounds from mulberry fruits. Korean J. Food Preserv", 11, pp.383~387 (2004)
- Koh, J.H, "Antioxidative and antimicrobial activities of pomegranate seed extracts Korean J. Food Cookery Sci", 21, pp.171~179 (2005)
- Kang, Y.H, "Studies on the physiological functionality of pine needle and mugwort extracts", Korean J. Food Sci. Technol, 27, pp.978~984
- Park, E.J, "Transgenic tomato plants that overexpress superoxide dismutase in fruits", Korean J. Plant Biotechnology, 29, pp.7~13 (2002)
- Hong, H.D, "Superoxide dismutase-like activity of apple juice mixed with some fruits and vegetables", Korean J. Food Sci. Technol, 30, pp.1484~1487 (1998)
- Lee, Y.J, "Antioxidative activities and nitrite scavenging abilities of extracts from Ulmus devidiana", J. Korean Soc. Food Sci. Nutr, 29, pp.893~899 (2000)
- Kang, Y. H, "Studies on the physiological functionality of pine needle and mugwort extracts", Korean J. Food Sci. Technol, 27, pp.978~984 (1995)
- 손종연, "석류씨 추출물의 항산화 및 항균활성", 한국식품조리과학회지, 21(2), pp.171~179 (2005), 한국식품조리과학회
- Moon, S.H., "Inhibiyory effects of xanthine oxidase by boiled water extracts and tannin from persimmon leaves", Korean J. Food and Nutr, 11, pp.354~357 (1998)
- Yeo, S.G, "Inhibition of xanthine oxidase by tea extracts from green tea, oolong tea and black tea", J. Korean Soc. Food Nutr, 24, pp.154~159 (1995)
- An, B.J., "Studies on Biological Activity from Extract of Crataegi Fructus", Korea J. Herbology, 17, pp.29~38 (2002)