유전자변형 배추를 섭취한 마우스 장기에서의 Housekeeping Gene의 발현 분석
Analysis of Housekeeping Gene Expression in Mice Administered to GM and non-GM Cabbage
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We used RT-PCR to measure housekeeping gene expression in mice fed GM and non-GM cabbage, in an effort to evaluate the risk of GM food to humans. After normalization of housekeeping gene levels, highly uniform expression may be seen in many organisms during various stages of development and under different environmental conditions. We assessed the expression of four genes in Chinese cabbage; these were Profilin, Tubulin-alpha (Tub-1), Heat-shock protein (Bchsp17.6), and Ubiquitin conjugating enzyme (UBE). We measured the expression of four well-known housekeeping genes in mice: β-actin (β-act), β-2-microglobulin (B2m), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-glucuronidase (Gus). Gene expression was measured in liver, stomach, small intestine, large intestine, kidney, and spleen of mice fed GM or non-GM cabbage. No significant expression differences were found.
Abstract
We used RT-PCR to measure housekeeping gene expression in mice fed GM and non-GM cabbage, in an effort to evaluate the risk of GM food to humans. After normalization of housekeeping gene levels, highly uniform expression may be seen in many organisms during various stages of development and under different environmental conditions. We assessed the expression of four genes in Chinese cabbage; these were Profilin, Tubulin-alpha (Tub-1), Heat-shock protein (Bchsp17.6), and Ubiquitin conjugating enzyme (UBE). We measured the expression of four well-known housekeeping genes in mice: β-actin (β-act), β-2-microglobulin (B2m), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-glucuronidase (Gus). Gene expression was measured in liver, stomach, small intestine, large intestine, kidney, and spleen of mice fed GM or non-GM cabbage. No significant expression differences were found.
참고문헌 (12)
- Blanco-Aparicio, C., "PTEN, more than the AKT pathway", Carcinogenesis, 28, pp.1379~1386 (2007)
- Tüting, T., "Genetically modified bone marrow-derived dendritic cells expressing tumor-associated viral or "self" antigens induce antitumor immunity in vivo", Eur. J. Immunol, 27, pp.2702~2707 (1997)
- Jank, B., "Co-existence of agricultural production systems", Trends Biotechnol, 24, pp.198~200 (2006)
- Reis, L.F., "GMOs: building the future on the basis of past experience", Ann. Acad. Bras. Cienc, 78, pp.667~686 (2006)
- Monastra, G, "Transgenic foods as a tool for malnutrition elimination and their impact on agricultural systems", Riv. Biol, 96, pp.363~384 (2003)
- Deaville, E.R., "Detection of transgenic and endogenous plant DNA fragments in the blood, tissues, and digesta of broilers", J. Agric. Food Chem, 53, pp.10268~10275 (2005)
- Gujar, G.T., "Helicoverpa armigera baseline susceptibility to Bacillus thuringiensis Cry toxins and resistance management for Bt cotton in India", J. Invertebr. Pathol, 95, pp.214~249 (2007)
- Jain, M., "Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR", Biochem. Biophys. Res. Commun, 345, pp.646~651 (2006)
- Filby, A.L., "Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish", BMC Mol. Biol, 8, pp.10~15 (2007)
- Rodriguez-Mulero, S., "Selection of a suitable internal control gene for expression studies in pancreatic islet grafts", Transplantation, 80, pp.650~652 (2005)
- Mazza, R., "Assessing the transfer of genetically modified DNA from feed to animal tissues", Transgenic Res, 14, pp.775~784 (2005)
- Guerrero, G.G., "Analysis of the cellular immune response induced by Bacillus thuringiensis Cry1A toxins in mice: effect of the hydrophobic motif from diphtheria toxin", Mol. Immunol, 44, pp.1209~1217 (2007)