해충저항성 GM감자와 non-GM감자의 Housekeeping gene 발현 분석
Analysis of Housekeeping Genes in Mice Feeding on GM and non-GM Potatoes
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초록
To develop human risk assessment protocols, we explored housekeeping gene and cytokine expression in mouse spleen cells using Rt-PCR. We normalized housekeeping gene expression by RT-PCR; gene expression was highly uniform in potato leafs and mouse spleen cells. We measured the expression of frequently used housekeeping genes, such as those encoding APRT, β-tubulin, Actin, Hsp 20.2, Cyclophilin, 18S RNA, Efla, Tbp, GAPDH, β-actin, Tuba2, Hprt, Cyclophlin A, Tfrc, and RPL13A in mice fed GM or non-GM potatoes. Housekeeping gene expression did not show any significant differences between GM and non-GM potato-fed mice. The murine model of potato-fed mice did not express IL-4 and IL-13 at a significant levels.
Abstract
To develop human risk assessment protocols, we explored housekeeping gene and cytokine expression in mouse spleen cells using Rt-PCR. We normalized housekeeping gene expression by RT-PCR; gene expression was highly uniform in potato leafs and mouse spleen cells. We measured the expression of frequently used housekeeping genes, such as those encoding APRT, β-tubulin, Actin, Hsp 20.2, Cyclophilin, 18S RNA, Efla, Tbp, GAPDH, β-actin, Tuba2, Hprt, Cyclophlin A, Tfrc, and RPL13A in mice fed GM or non-GM potatoes. Housekeeping gene expression did not show any significant differences between GM and non-GM potato-fed mice. The murine model of potato-fed mice did not express IL-4 and IL-13 at a significant levels.
참고문헌 (21)
- Pinheiro, M.M., "A technology with multiple applications", Hist. Cienc. Saude Manguinhos, 7, pp.465~479 (2000)
- Sakamoto, T., "Generating high-yielding varieties by genetic manipulation of plant architecture", Curr. Opin. Biotechnol, 15, pp.144~147 (2004)
- Zhu, C., "Transgenic strategies for the nutritional enhancement of plants", Trends Plant Sci, 12, pp.548~555 (2007)
- Campbell, M.A., "Engineering pathogen resistance in crop plants", Transgenic Res, 11, pp.599~613 (2002)
- Mazza, R., "Assessing the transfer of genetically modified DNA from feed to animal tissues", Transgenic Res, 14, pp.775~784 (2005)
- Guerrero, G.G., "Analysis of the cellular immune response induced by Bacillus thuringiensis Cry1A toxins in mice: effect of the hydrophobic motif from diphtheria toxin", Mol. Immunol, 44, pp.1209~1217 (2007)
- Oliveira, A.R., "Toxicological evaluation of genetically modified cotton (Bollgard) and Dipel WP on the non-target soil mite Scheloribates praeincisus (Acari: Oribatida)", Exp. Appl. Acarol, 41, pp.191~201 (2007)
- O'Callaghan, M., "Effects of plants genetically modified for insect resistance on nontarget organisms", Ann. Rev. Entomol, 50, pp.271~292 (2005)
- Dale, PJ., "Potential for the environmental impact of transgenic crops", Nat. Biotechnol, 20, pp.567~574 (2002)
- Sinha, G., "AGBIOTECH. GM technology develops in the developing world", Science, 315, pp.182~183 (2007)
- Vain, P., "Global trends in plant transgenic science and technology (1973-2003)", Trends Biotechnol, 24, pp.206~211 (2006)
- 조현석, "주요 유전자변형작물(벼, 배추, 감자, 잔디)의 환경 위해성 평가 연구", pp.1~350 (2008), 농촌진흥청
- Heo, J.C., "Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity", J. Ethnopharmacol, 102, pp.10~14 (2005)
- Murai, A., "Improvement of growth and nutritive value in chicks with non-genetically modified phytase product from Aspergillus niger", Br. Poult. Sci, 43, pp.687~695 (2002)
- Davies, K.M., "Genetic modification of plant metabolism for human health benefits", Mutat. Res, 622, pp.122~137 (2007)
- Krayer von Krauss, M.P., "Elicitation of expert judgments of uncertainty in the risk assessment of herbicide-tolerant oilseed crops", Risk Anal, 24, pp.1515~1527 (2004)
- Schrøder, M., "A 90-day safety study of genetically modified rice expressing Cry1Ab protein (Bacillus thuringiensis toxin) in Wistar rats", Food Chem. Toxicol, 45, pp.339~349 (2007)
- Soberón, M., "Engineering modified Bt toxins to counter insect resistance", Science, 318, pp.1640~1642 (2007)
- Akiyama, H., "Individual detection of genetically modified maize varieties in non-identity-preserved maize samples", J. Agric. Food Chem, 56, pp.1977~1983 (2008)
- Yum, H.Y., "Genetically modified and wild soybeans: an immunologic comparison", Allergy Asthma Proc, 26, pp.210~216 (2005)
- Gizzarelli, F., "Evaluation of allergenicity of genetically modified soybean protein extract in a murine model of oral allergen-specific sensitization", Clin. Exp. Allergy, 36, pp.238~248 (2006)