마이크로웨이브 추출조건에 따른 백지 추출물의 폴리페놀 함량과 항산화 작용
Polyphenol Contents and Antioxidant Activity of Extracts from Angelica dahurica Root after Different Conditions ofMicrowave-assisted Extraction
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초록
This study investigated the antioxidant activities of extracts from Angelica dahurica roots after microwave-assisted extraction with different levels of energy (120, 240 W) and extraction time (5, 10, 15 and 30 min). The highest extraction yield was 11.77 mg% in water at 240 W for 30 min followed by 11.42 mg% in water at 120 W and 30 min. The highest total polyphenol contents was 32.36 mg/g in an ethanol extract, followed by 31.77 mg/g in water extract at the same conditions of 240 W, 30 min. The electron donating abilities both the ethanol extract obtained using 240 W and 30 min and the water extract obtained employing 120 W and 5 min showed the highest values, 83.55% and 82.49% respectively at a concentration 1.0 mg/mL. The highest superoxide dismutase (SOD)-like activity was 14.16% in ethanol extract at 120 W and 15 min, followed by 13.22% in the water extract at 120 W and 5 min. The best extraction yield and polyphenol content after microwave-assisted extraction were achieved with 240 W and 30 min using water. The best condition for extraction of electron donating ability and SOD-like activity from A. dahurica roots were 120 W and 5 minutes using water.
Abstract
This study investigated the antioxidant activities of extracts from Angelica dahurica roots after microwave-assisted extraction with different levels of energy (120, 240 W) and extraction time (5, 10, 15 and 30 min). The highest extraction yield was 11.77 mg% in water at 240 W for 30 min followed by 11.42 mg% in water at 120 W and 30 min. The highest total polyphenol contents was 32.36 mg/g in an ethanol extract, followed by 31.77 mg/g in water extract at the same conditions of 240 W, 30 min. The electron donating abilities both the ethanol extract obtained using 240 W and 30 min and the water extract obtained employing 120 W and 5 min showed the highest values, 83.55% and 82.49% respectively at a concentration 1.0 mg/mL. The highest superoxide dismutase (SOD)-like activity was 14.16% in ethanol extract at 120 W and 15 min, followed by 13.22% in the water extract at 120 W and 5 min. The best extraction yield and polyphenol content after microwave-assisted extraction were achieved with 240 W and 30 min using water. The best condition for extraction of electron donating ability and SOD-like activity from A. dahurica roots were 120 W and 5 minutes using water.
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