감귤의 고미제거 효소인 limonoid UDP-glucosyltransferase의 대장균 내에서의 발현과 이의 분리
Expression and Isolation of Limonoid UDP-glucosyltransferase, a Bitterness-reducing Enzyme, in E.coli
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Limonoids are abundant as bitter taste in citrus fruit and other plants. Interestingly, limonoid UDP-glucosyltransferase (LUGT) effectively ameliorates the bitterness from limonoid. The high level of LUGT expression in Escherichia coli can result in the formation of insoluble aggregates known as inclusion bodies. We isolated the soluble LUGT protein when this inclusion body was renaturated with β-cyclidextrin treatment after protein denaturation by urea. Our present results suggest that the isolation of LUGT from inclusion body in cells leads to shed light to characterize the enzyme for food industry purposes.
Abstract
Limonoids are abundant as bitter taste in citrus fruit and other plants. Interestingly, limonoid UDP-glucosyltransferase (LUGT) effectively ameliorates the bitterness from limonoid. The high level of LUGT expression in Escherichia coli can result in the formation of insoluble aggregates known as inclusion bodies. We isolated the soluble LUGT protein when this inclusion body was renaturated with β-cyclidextrin treatment after protein denaturation by urea. Our present results suggest that the isolation of LUGT from inclusion body in cells leads to shed light to characterize the enzyme for food industry purposes.
참고문헌 (13)
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